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Mainstream Attached Growth Partial Nitritation and Anammox: Design and OptimizationIkem, Juliet Ogochukwu 01 December 2023 (has links)
There is a significant need to remove ammonia from municipal wastewater to meet increasingly stringent regulations set by Canada, US, and Europe. Although existing conventional biological wastewater treatment technologies are shown to achieve effective ammonia treatment, they are substantially limited by increased operational intensity and cost. Due to these limitations, other cost-effective biological treatment technologies, such as partial nitritation/anammox (PN/A), have become a more attractive solution for nitrogen removal at wastewater resource recovery facilities (WRRF). A moving bed biofilm reactor system (MBBR) operating under a novel design strategy using elevated total ammonia nitrogen (TAN) loading rate has shown promise to achieve robust partial nitritation and the oxidation of TAN with limited oxidation of nitrite without the need for intense operational measures. However, the novel and promising design strategy using elevated TAN loading rate was applied at higher influent TAN concentrations that are typically greater than concentrations in mainstream municipal wastewater. Therefore, the objective of this dissertation is to investigate and optimize the design and performance of a promising elevated loaded partial nitritation MBBR technology for mainstream, municipal wastewater treatment followed by downstream anammox to complete the design of a robust, stable, energy-efficient, and low operational cost total nitrogen removal PN/A system for mainstream wastewaters. The first specific objective of the dissertation is to isolate the optimal design parameter of a mainstream elevated loaded partial nitritation MBBR system. The results identifies optimal distinct elevated surface area loading rate (SALR), hydraulic retention time (HRT), and airflow rate that achieve stable partial nitritation performance (i.e., optimum total ammonia nitrogen (TAN) removal kinetics and percent NOₓ as nitrite) in a mainstream elevated loaded partial nitritation MBBR system. The study shows that TAN SALR, HRT, and airflow rate significantly affect TAN surface area removal rates (SARR) and percent NOₓ as nitrite and, as such, identifies the optimal design parameters (TAN SALR, HRT and airflow rate) of a mainstream elevated loaded partial nitritation MBBR system. A TAN SALR of 5 g TAN/m²∙d, HRT of 2h and airflow rate of 1.5 L/min are identified to provide stable partial nitritation performance with a TAN SARR of 2.3 ± 0.3 g TAN/m²∙d and a percent of NOx as nitrite of 84.8 ± 1.2% in the mainstream elevated loaded partial nitritation MBBR system. The second specific objective further identifies a new design configuration and the mechanism of nitrite oxidation suppression of the mainstream elevated loaded partial nitritation MBBR technology. The results identifies a unique design strategy using an elevated TAN SALR of 5 g TAN/m²∙d to achieve cost-effective, stable, and elevated rates of partial nitritation in an MBBR system under mainstream conditions. The elevated loaded partial nitritation MBBR system achieves a TAN SARR of 2.01 ± 0.1 g TAN/m²∙d and NO₂⁻-N:NH₄⁺-N stoichiometric ratio of 1.15:1, which is appropriate for downstream anammox treatment. The elevated TAN SALR design strategy promotes nitrite-oxidizing bacteria (NOB) activity suppression rather than a reduction in NOB population as the reason for the suppression of nitrite oxidation in the mainstream elevated loaded partial nitritation MBBR system. NOB activity is limited at an elevated TAN SALR, likely due to thick biofilm embedding the NOB population and competition for dissolved oxygen (DO) with ammonia-oxidizing bacteria for TAN oxidation to nitrite within the biofilm structure, which ultimately limits the uptake of DO by NOB in the system. The third specific objective of this research characterizes the effects of distinct mixing and aeration strategies on the performance of the mainstream elevated loaded partial nitritation MBBR technology. This is addressed through a study investigating and comparing the kinetics, biofilm characteristics, and embedded biomass of three distinct mixing and aeration strategies employed to operate the mainstream elevated loaded partial nitritation MBBR system. The study compares the conventional mixing and aeration condition, continuous aeration with mechanical paddle & aeration, and recirculation pump & aeration utilized to optimize the partial nitritation MBBR system to achieve low DO effluent concentrations for optimal downstream anammox treatment. The results show that maintaining mixing and aeration in the elevated loaded partial nitritation MBBR system with recirculation pump & reduced aeration achieves lower effluent DO concentration and stable partial nitritation with appropriate NO₂⁻-N:NH₄⁺-N stoichiometry ratio of 1.09:1 for subsequent anammox treatment compared to operation with continuous aeration or mechanical paddle & aeration. The fourth specific objective of this research investigates the promising elevated loaded PN/A configured system for nitrogen removal under mainstream conditions. This is achieved through the operation of the elevated loaded partial nitritation MBBR system following the anammox unit as a combined two-stage system for nitrogen removal at mainstream municipal concentration. The elevated loaded partial nitritation MBBR system provides optimal NH₄⁺-N:NO₂⁻-N stoichiometric effluent ratio of 1:1.17, resulting in the successful operation of a downstream anammox unit with a total nitrogen removal rate at 0.22 ± 0.2 g N/m²/d and total nitrogen removal efficiency at 74.1 ± 0.7%. The average NO₂⁻-N to NH₄⁺-N molar removal ratio is 1.05 ± 0.1 from the anammox unit. Also, the anammox bacteria (AnAOB) gene copies are at 3.28 ± 0.7 × 10⁸, a value significantly higher than the AOB and NOB gene copies at 9.17 ± 1.1 × 10⁴ and 6.23 ± 1.0, respectively. This confirms that anammox activity is established and nitrogen removal is primarily through the anammox process. The results and overall system performance demonstrate that the combined two-stage mainstream elevated loaded partial nitritation/anammox MBBR system has shown promise and offers great insights for further advancement of the anammox process at mainstream municipal wastewaters. Finally, the economic evaluation and cost comparative analyses conducted show that compared to the conventional biological nitrification/denitrification process for nitrogen removal, the two-stage elevated loaded PN/A system offers a 57.6% savings on energy cost, 100% savings on chemical cost, and 68.7% savings on the cost of sludge disposal. Therefore, the two-stage elevated loaded PN/A system, in addition to high nitrogen removal efficiency, reduced footprint, and ease of operation, is also economically favorable and reduces the overall operational cost of wastewater treatment system by 61.6%, thus saving up to an average of 3.7 million CAD every year.
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The Response of Wild Fish to Municipal Wastewater Effluent Exposures at Sites in CanadaTetreault, Gerald 08 March 2012 (has links)
Aquatic receiving environments have long been used to dilute municipal wastewater effluents (MWWE) which are the largest discharge by volume into the aquatic environment in Canada. These treated effluents are a complex mixture of environmental contaminants that includes natural and synthetic hormones, pharmaceuticals, industrial chemicals, nutrients and ammonia. Discharge of MWWE may lead to serious problems in aquatic environments such as eutrophication, hypoxia as well as increased occurrence of disease and toxicity in resident aquatic biota. Reproductive impairment in fish has also been widely reported in association with exposure to wastewaters. Recently, concerns have been raised about the potential for municipal effluents to cause endocrine disruption in fish and other organisms. The effects of MWWE on fish and fish populations in Canada are currently poorly understood. The overall objective of this thesis is to contrast the impact of MWWE discharged into two Canadian rivers on sentinel fish species across levels of biological organization ranging from biochemical responses to changes at the fish community level. Results from these studies support the development of robust effects-based biological monitoring approaches to assess the effectiveness of regulations and remedial actions for minimizing the effects of MWWE.
Understanding the temporal changes in physiological and reproductive parameters across the annual cycle of a sentinel species is necessary to optimize biomonitoring programs. The annual variability in terms of survival, reproduction and energy storage in the Greenside Darter (Etheostoma blennioides), a potential sentinel species for the Grand River, Ontario, was documented at a reference site across two years. Variation in energy storage and reproductive development indicated by somatic indices (i.e., relative organ size) and steroid production suggest that biomonitoring can be optimized for this species by sampling in late fall or early spring (pre-spawning). With this new knowledge, field studies conducted with small bodied species, including Rainbow Darter (E. caeruleum), Brook Stickleback (Culaea inconstans) and Fathead Minnow (Pimephales promelas) have demonstrated that, when sampled during the appropriate season (e.g., pre-spawning), they can be used as effective biomonitoring tools to detect fish responses associated with exposure to MWWEs.
Two sentinel fish species, Rainbow Darter and Greenside Darter, were then used to examine the impact of two MWWE discharges on fish in the Grand River, Ontario, relative to reference sites in two seasons (fall and early spring). Fish responses, in terms of energy storage (condition factor, liver size), energy utilization (gonadosomatic indices) and reproduction (in vitro sex steroid production, cellular development and intersex) were assessed at each site. Both sentinel species were longer and heavier downstream of the wastewater outfalls. However, these larger fish did not demonstrate consistent increases in condition and liver somatic indices. MWWE-exposed male Rainbow and Greenside Darters had impaired capacity to produce androgens in vitro, lower gonadosomatic indices and altered sperm cell staging. Exposed female fish also had impaired capacity to produce estrogens in vitro, however, they did not demonstrate differences in oocyte development. Male Rainbow and Greenside Darters collected downstream of both MWWE discharges showed increased incidence of intersex (33 - 100%) in contrast to very low occurrences of this condition in upstream agricultural and urban reference sites. This increased incidence of intersex coincided with reductions in gonadosomatic indices and capacity to produce steroids, demonstrating the ability of MWWE to alter the reproductive systems of these fish. The fish communities downstream of the the MWWE outfalls demonstrated differences in abundance, diversity, and species composition when compared to reference sites. MWWE exposed sites had few of the darter species that dominate the fish community at reference sites. More mobile fish species such as suckers (Catostomidae spp.) and sunfish (Centrarchidae spp.) were more common downstream of the outfalls, with occurances becoming more pronounced downstream of the second sewage discharge.
Wascana Creek, Saskatchewan, downstream of the wastewater treatment plant for the City of Regina can be up to 100% treated municipal wastewater. Brook Stickleback and Fathead Minnow exhibited delayed spawning and altered gonadal development downstream of the wastewater outfall. Exposed male Fathead Minnows were feminized, having lower expression of secondary sexual characteristics (i.e., loss of nuptial tubercles, dorsal pad, and dorsal fin dot) and induction of the female egg-yolk precursor protein, vitellogenin. Fathead Minnows also showed cellular damage to the gills and kidneys. These responses indicate exposure to a variety of environmental contaminants in the effluent such as ammonia as well as endocrine disruptors.
The potential effect of MWWE discharges in these two Canadian watersheds on fish responses was demonstrated across various levels of biological organization including reduced sex steroid production, altered gonadal development, reduction in gonadosomatic indices, delayed spawning, and changes in fish assemblages. An effects-based monitoring approach using sentinel species can be successfully applied to detect changes associated with MWWE outfalls, as long as sampling of sentinel species is conducted during optimal time periods (i.e., when somatic indices are maximized and variability among individuals is minimized). MWWE can impair the reproductive potential of fish beyond a threshold where impacts are expressed at higher levels of organization such as populations or communities. It is essential to make mechanistic linkages between responses at different levels to determine the overall potential impact of effluents on fish. The collection of responses across multiple levels of biological organization can complement and support development of biomonitoring approaches that are focused at the population and community levels such as those being proposed for MWWE in Canada.
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Developing an Understanding for Wastewater Treatment in Remote Communities in Nunavut, Canada: Investigating the Performance, Planning Practice and Function of Tundra and Constructed Treatment WetlandsYates, Colin Nathan 06 November 2014 (has links)
Since humans began to permanently settle locations for extended periods of time there has been the challenge to safely dispose of, or treat human effluent. In specific to the communities of Nunavut and Arctic Canada, the treatment of wastewater has been particularly challenging. The harsh climate, remote nature and socio-economic factors are a few of the aspects which make the treatment of wastewater problematic in Canadian Arctic communities.
In the past several decades a number of conventional and alternative wastewater treatment systems (e.g. lagoons and tundra wetlands) have been proposed and implemented in Nunavut and other remote Arctic communities. Knowledge of performance of these systems is limited, as little research has been conducted and regulatory monitoring has been poorly documented or not observed at all. Also, in the past, the rational design process of treatment systems in Arctic communities has not acknowledged cultural and socio-economic aspects, which are important for the long-term management and performance of the treatment facilities in Arctic communities.
From 2008 to 2010 I characterized and studied the performance of several tundra wastewater treatment wetlands in the Kivalliq Region of Nunavut, as well as two in the Inuvaliat Region of the Northwest Territories. Performance testing occurred weekly throughout the summer of 2008. Characterization included surveys of plant communities in the tundra wetlands, specifically analyzing the relationship between Carex aquatilis and various nutrient contaminants in wastewater. Through their characterization I was able to provide greater insight into primary treatment zones within the wetland, and identify the main potential mechanisms for the treatment wastewater in the Arctic. I also studied the performance of a horizontal subsurface flow (HSSF) constructed wetland in Baker Lake Nunavut; the first system of its kind in the Canadian Arctic.
The weekly performance study showed average weekly percent reduction in all parameters, with small deviations immediately after snow-melt and at the beginning of freeze-up. For the six parameters monitored I observed reductions of 47-94% cBOD5, 57-96% COD, 39-98% TSS, >99% TC, >99% E. coli, 84-99% NH3-Nand 80-99% TP for the six tundra treatment wetlands. Whereas, the wetland characterization study through the use of spatial interpolations on each of the wetlands and their water quality showed that concentrations of the wastewater parameters decreased the most in the first 100m of the wetland in all three treatment wetlands used in this portion of the analysis (Chesterfield Inlet, Paulatuk and Ulukhaktok). Areas of greatest concentration where shown to follow preferential flow paths with concentrations decreasing in a latitudinal and longitudinal direction away from the wastewater source. The Paulatuk and Ulukhaktok treatment wetlands were observed to effectively polish pre-treated wastewater from the facultative lake and engineered lagoon, with removals of key wastewater constituents of cBOD5, TSS and NH3-N to near background concentrations. And despite the absence of pre-treatment in Chesterfield Inlet, the wetland was also observed to effectively treat wastewater to near background concentrations. Further characterization on the composition of the sedge C. aquatilis, showed a high percent cover of the species corresponded with areas of high concentration of NH3-N in the wastewater. A principal components analysis verified the spatial results showing correlation between C. aquatilis cover and NH3-N concentrations. Analysis also showed strong positive relationship between sites closer to the source of wastewater and C. aquatilis. No correlation was found between the other parameters analyzed and C. aquatilis.
The first year of study of the HSSF constructed wetland showed promising mean removals in cBOD5, COD, TSS, E. coli, Total Coliforms, and TP throughout the summer of
2009; removals of 25%, 31%, 52%, 99.3%, 99.3%, and 5% were observed respectively. However, the second year of study in 2010 the system did not perform as expected, and concentrations of effluent actually increased. I concluded that a high organic loading during the first year of study saturated the system with organics.
Finally, a review of planning process and regulatory measures for wastewater in Arctic communities and the impending municipal wastewater standards effluent resulted in the following recommendations; i) wastewater effluent standards should reflect the diverse arctic climate, and socio-economic environment of the northern communities, ii) effluent standards should be region or even community specific in the Arctic, and iii) for planning and management of wastewater incorporation of Inuit understanding of planning and consultation needs to be incorporated in the future.
This research has several major implications for wastewater treatment and planning for Nunavut and other Arctic Regions. The performance and characterization of tundra treatment wetlands fills significant gaps in our understanding of their performance and potential mechanisms of treatment, and treatment period in the Kivalliq Region. Although the HSSF constructed wetland failed, further research into engineered/augmented treatment wetlands should be considered as they provide low-cost low maintenance solutions for remote communities. Finally, the data collected in this study will provide significant insight into the development of new municipal wastewater effluent standards for northern communities, which will be reflected in the Fisheries Act.
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The Response of Wild Fish to Municipal Wastewater Effluent Exposures at Sites in CanadaTetreault, Gerald 08 March 2012 (has links)
Aquatic receiving environments have long been used to dilute municipal wastewater effluents (MWWE) which are the largest discharge by volume into the aquatic environment in Canada. These treated effluents are a complex mixture of environmental contaminants that includes natural and synthetic hormones, pharmaceuticals, industrial chemicals, nutrients and ammonia. Discharge of MWWE may lead to serious problems in aquatic environments such as eutrophication, hypoxia as well as increased occurrence of disease and toxicity in resident aquatic biota. Reproductive impairment in fish has also been widely reported in association with exposure to wastewaters. Recently, concerns have been raised about the potential for municipal effluents to cause endocrine disruption in fish and other organisms. The effects of MWWE on fish and fish populations in Canada are currently poorly understood. The overall objective of this thesis is to contrast the impact of MWWE discharged into two Canadian rivers on sentinel fish species across levels of biological organization ranging from biochemical responses to changes at the fish community level. Results from these studies support the development of robust effects-based biological monitoring approaches to assess the effectiveness of regulations and remedial actions for minimizing the effects of MWWE.
Understanding the temporal changes in physiological and reproductive parameters across the annual cycle of a sentinel species is necessary to optimize biomonitoring programs. The annual variability in terms of survival, reproduction and energy storage in the Greenside Darter (Etheostoma blennioides), a potential sentinel species for the Grand River, Ontario, was documented at a reference site across two years. Variation in energy storage and reproductive development indicated by somatic indices (i.e., relative organ size) and steroid production suggest that biomonitoring can be optimized for this species by sampling in late fall or early spring (pre-spawning). With this new knowledge, field studies conducted with small bodied species, including Rainbow Darter (E. caeruleum), Brook Stickleback (Culaea inconstans) and Fathead Minnow (Pimephales promelas) have demonstrated that, when sampled during the appropriate season (e.g., pre-spawning), they can be used as effective biomonitoring tools to detect fish responses associated with exposure to MWWEs.
Two sentinel fish species, Rainbow Darter and Greenside Darter, were then used to examine the impact of two MWWE discharges on fish in the Grand River, Ontario, relative to reference sites in two seasons (fall and early spring). Fish responses, in terms of energy storage (condition factor, liver size), energy utilization (gonadosomatic indices) and reproduction (in vitro sex steroid production, cellular development and intersex) were assessed at each site. Both sentinel species were longer and heavier downstream of the wastewater outfalls. However, these larger fish did not demonstrate consistent increases in condition and liver somatic indices. MWWE-exposed male Rainbow and Greenside Darters had impaired capacity to produce androgens in vitro, lower gonadosomatic indices and altered sperm cell staging. Exposed female fish also had impaired capacity to produce estrogens in vitro, however, they did not demonstrate differences in oocyte development. Male Rainbow and Greenside Darters collected downstream of both MWWE discharges showed increased incidence of intersex (33 - 100%) in contrast to very low occurrences of this condition in upstream agricultural and urban reference sites. This increased incidence of intersex coincided with reductions in gonadosomatic indices and capacity to produce steroids, demonstrating the ability of MWWE to alter the reproductive systems of these fish. The fish communities downstream of the the MWWE outfalls demonstrated differences in abundance, diversity, and species composition when compared to reference sites. MWWE exposed sites had few of the darter species that dominate the fish community at reference sites. More mobile fish species such as suckers (Catostomidae spp.) and sunfish (Centrarchidae spp.) were more common downstream of the outfalls, with occurances becoming more pronounced downstream of the second sewage discharge.
Wascana Creek, Saskatchewan, downstream of the wastewater treatment plant for the City of Regina can be up to 100% treated municipal wastewater. Brook Stickleback and Fathead Minnow exhibited delayed spawning and altered gonadal development downstream of the wastewater outfall. Exposed male Fathead Minnows were feminized, having lower expression of secondary sexual characteristics (i.e., loss of nuptial tubercles, dorsal pad, and dorsal fin dot) and induction of the female egg-yolk precursor protein, vitellogenin. Fathead Minnows also showed cellular damage to the gills and kidneys. These responses indicate exposure to a variety of environmental contaminants in the effluent such as ammonia as well as endocrine disruptors.
The potential effect of MWWE discharges in these two Canadian watersheds on fish responses was demonstrated across various levels of biological organization including reduced sex steroid production, altered gonadal development, reduction in gonadosomatic indices, delayed spawning, and changes in fish assemblages. An effects-based monitoring approach using sentinel species can be successfully applied to detect changes associated with MWWE outfalls, as long as sampling of sentinel species is conducted during optimal time periods (i.e., when somatic indices are maximized and variability among individuals is minimized). MWWE can impair the reproductive potential of fish beyond a threshold where impacts are expressed at higher levels of organization such as populations or communities. It is essential to make mechanistic linkages between responses at different levels to determine the overall potential impact of effluents on fish. The collection of responses across multiple levels of biological organization can complement and support development of biomonitoring approaches that are focused at the population and community levels such as those being proposed for MWWE in Canada.
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Cultivation of Oleaginous Microorganism Consortium on Municipal Wastewater for the Production of LipidsHall, Jacqueline Isonhood 12 May 2012 (has links)
Alternative fuels are necessary to meet the increasing demands for fuels. Alternative fuels such as biodiesel are produced using vegetable oils, which are prominentt in the food industry. An alternate feedstock could be oil-producing microorganisms. These oleaginous microorganisms are defined as accumulating more than 20% of their weight in oil as lipids. Cultivating these microorganisms for oil production is not economical due to the high production costs from the sugars in the culture medium. Municipal wastewater could be a potential growth medium that has not previously been considered for cultivating oleaginous microorganisms. However, municipal wastewater contains a low concentration of carbon, which does not promote oil accumulation in the oleaginous microorganisms. To increase the carbon concentration in the wastewater, lignocellulosic sugars could be added to the municipal wastewater. These sugars are a potential alternative to sugars that are in the food industry. The goal of this research is to determine the efficacy of using municipal wastewater to cultivate a consortium of oleaginous microorganisms, thus, producing oil for biodiesel production. First, a consortium of oleaginous microorganisms was cultivated on autoclaved wastewater to determine if the wastewater contains any inhibiting substances for the microorganisms. In addition to the substances in the wastewater, indigenous microorganisms are possible inhibitors to the consortium. Therefore, to determine the effect these indigenous microorganisms have on the oleaginous microorganisms, the consortium was cultivated on raw municipal wastewater amended with varying amounts of sugar. Since the municipal wastewater can be used as a cultivation medium, the effect of the addition of lignocellulosic sugars was determined. During the production of lignocellulosic sugars, furfural and acetic acid, known microbial inhibitors, are formed. The effect of these inhibitors on the consortium’s growth and oil accumulation ability was ascertained, and inhibition models were developed to describe their impact. With these results, SuperPro Designer v6.0 was used to perform simulations and economic analyses to determine the efficacy of incorporating an oleaginous microorganism consortium in a wastewater treatment facility.
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Microbiomes of freshwater insects and riparian spiders downstream of municipal wastewater discharges in the Bow River, ABDiesbourg, Emilie January 2024 (has links)
The host microbiome (mainly bacteria) is essential for host immune function, metabolism, and digestion. Alterations in these microbes, known as dysbiosis, generally results in adverse effects to the host, including diseases. Dysbiosis can be induced from exposures to various anthropogenic contaminants including constituents of municipal wastewater treatment effluents (MWWEs), namely, pharmaceuticals, antibiotics, and excess nutrients. Despite MWWEs being one of the largest dischargers to aquatic ecosystems, impacts of these contaminants on exposed organism microbiomes, especially in aquatic insects, is unclear. In addition, some aquatic contaminants may transfer to riparian habitats through predation on emergent insects that were exposed to contaminants as larvae, and subsequently alter microbiomes of terrestrial predators. Our study evaluated whether MWWEs altered microbiomes of freshwater larval and adult insects and their riparian spider predators using effluent-associated bacteria and stable nitrogen isotopes (δ15N) to confirm effluent exposure. We analyzed microbiome compositions through sequencing of the V3-V4 hypervariable region of the 16S rRNA gene and analyzed food web dynamics with stable carbon (δ13C) and nitrogen (δ15N) isotopes. We found that insects and spiders were enriched in δ15N at one site downstream of wastewater outfalls, indicating exposure to effluents and transfer of nutrients to terrestrial ecosystems. Microbiomes of most larval and adult insects were altered downstream of wastewater outfalls and had lower relative abundances of endosymbiont bacteria, shifts in bacterial diversities, increases in abundances of effluent-associated bacteria, and downregulation of some biosynthesis pathways than those collected at upstream sites. However, spider microbiomes had little evidence of dysbiosis, and were distinct from those of adult insects, despite a close association in their isotopic signatures. Overall, this study provides evidence of biological impacts from MWWEs to exposed insects and suggests that changes in microbial communities of invertebrates may be used as an effective indicator of effluent exposure as part of monitoring frameworks. / Thesis / Master of Science (MSc) / Municipal wastewater treatment facilities release contaminants into aquatic ecosystems that may affect the health of exposed organisms, including their microbiome, which contains bacteria essential for host digestion and immune function. Alterations in microbiomes of contaminant-exposed aquatic insects are poorly characterized and such effects may be transferred to terrestrial ecosystems through emergent insects. This study evaluated microbiomes and food web dynamics of freshwater insects and riparian spiders upstream and downstream of wastewater treatment facilities in the Bow River, AB. Results indicate that microbiomes of some downstream aquatic insects had lower relative abundances of endosymbiont bacteria, shifts in bacterial diversities, and increases in abundances of effluent-associated bacteria than those collected at upstream sites, but no such changes were observed in the spider predators. This study improves our understanding of how freshwater insect microbiomes are altered by municipal wastewater effluents and suggests that directly effluent-exposed organisms are more at risk of dysbiosis.
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Stress and metabolic responses to municipal wastewater effluent exposure in rainbow trout effluentIngs, Jennifer Sophia January 2011 (has links)
Municipal wastewater effluent (MWWE) is an important source of pollution in the aquatic environment impacting fish. MWWE is a complex mixture of chemicals including pharmaceuticals, personal care products, industrial chemicals and pesticides. A link between reproductive endocrine disruption and MWWE exposure has been established in fish, but less is known about the effects of MWWE on non-reproductive endocrine disruption. The overall objective of this thesis was to examine the impacts of MWWE exposure on the stress response and intermediary metabolism in rainbow trout (Oncorhynchus mykiss). In fish, the primary adaptive organismal stress response involves the activation of hypothalamic-sympathetic-chromaffin axis to produce catecholamines, predominantly epinephrine, and the hypothalamic-pituitary-interrenal (HPI) axis to produce cortisol. Both of these hormones play a key role in elevating plasma glucose levels that is essential to fuel the increased energy demand associated with stress. Along with the organismal stress response, the cellular stress response, involving the synthesis of a suite of heat shock proteins (hsps), also plays an important role in protecting cellular protein homeostasis in response to stressors, including toxicants.
The impact of MWWE on stress-related pathways were identified using a low-density trout cDNA microarray enriched with genes encoding for proteins involved in endocrine-, stress- and metabolism-related processes. This was further confirmed by assessing plasma hormone and metabolite levels and stress-related targeted genes and proteins expression and enzyme activities in select tissues in rainbow trout. Studies were carried out in controlled field (caging) and laboratory experiments to examine the impacts of MWWE on stress and tissue-specific metabolic responses in rainbow trout. Further in vitro studies using rainbow trout hepatocytes in primary cultures were carried out to investigate the mechanism of action of two pharmaceuticals, atenolol and venlafaxine, found in relatively high concentrations in MWWE in impacting the stress-mediated glucose response.
In caged fish, MWWE exposure significantly elevated plasma cortisol and glucose concentrations, and altered the mRNA abundance of a number of stress-related genes, hormone receptors, glucose transporter 2 and genes related to immune function. When fish were exposed to an acute handling stress following a 14 d exposure to MWWE, the cortisol response was abolished and the glucose response was attenuated. The effects on cortisol did not correlate with changes in the expression of genes involved in cortisol biosynthesis, but were associated with an increase in hepatic glucocorticoid receptor (GR) protein expression.
Upon further investigation in controlled laboratory studies, MWWE exposure elevated constitutive hsp 70 and hsp90 expression after 8 d exposure, which correlated with a decrease in glycogen levels in the liver in fish exposed to a high concentration of MWWE compared to control fish, pointing to a MWWE-induced increase in liver energy demand. By 14 d, glycogen stores were replenished, and this was commensurate with increases in liver gluconeogenic capacity, including increases in the activities of phosphoenolpyruvate carboxykinase (PEPCK) and alanine aminotransferase (AlaAT), along with a decrease in liver GR expression. In the heart, GR protein expression increased in treated fish, and the activity of pyruvate kinase increased, indicating an increase in glycolytic capacity. Subjecting the MWWE exposed fish to a secondary handling disturbance (acute stress) led to an attenuated plasma cortisol and glucose response compared to the control group. This corresponded with a reduced liver gluconeogenic capacity and a lower liver and heart glycolytic capacities, reflecting a disturbance in the energy substrate repartitioning that is essential to cope with stress.
While it is difficult to establish causative agents from a complex mixture such as MWWE, the two pharmaceutical that were tested impacted glucose production. Specifically, atenolol and venlafaxine disrupted the epinephrine-induced glucose production, but did not modify cortisol-mediated glucose production in trout hepatocytes. The suppression of epinephrine-mediated glucose production by atenolol and venlafaxine was abolished by cAMP analogue (8-bromo cAMP) or glucagon (a metabolic hormone that increases glucose production). This suggests that both drugs disrupt β-adrenoceptor signaling, while it remains to be determined if the response is receptor isoform-specific.
Altogether MWWE exposure disrupts the organismal and cellular stress responses in trout. Key targets for MWWE impact leading to the impaired cortisol and metabolic responses to stress include liver and heart GR expression, liver gluconeogenic capacity, and liver, heart and gill glycolytic capacities. Most significantly, MWWE impairs the ability to metabolically adjust to a secondary acute stressor, which is an important adaptive process that is integral to successful stress performance. From an environmental stand-point, long-term exposure to MWWE will lead to reduced fitness and will compromise the capacity of fish to cope with additional stressor, including escape from predators.
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Anaerobic codigestion of municipal wastewater sludge and restaurant greaseLiu, Zengkai Unknown Date
No description available.
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Thyroid Hormone Disrupting Effects of Municipal WastewaterWojnarowicz, Pola 10 December 2013 (has links)
Current municipal wastewater treatment plants (MWWTP) technologies are insufficiently removing emerging contaminants of concern. These emerging contaminants are an issue as many are known endocrine disrupting compounds (EDCs). EDCs are contaminants that can have severe and irreversible impacts on highly conserved endocrine systems that are critical during developmental periods in vertebrates as well as during adult life. Many EDCs have non-monotonic dose-response curves yet they are not often tested at low, environmentally relevant concentrations. EDC research to date has focused heavily on xenoestrogenic compounds whereas thyroid hormone (TH) disruption has been largely overlooked.
TH is conserved in all vertebrates and plays crucial roles in neural development, basal metabolism, and thermoregulation. TH is comprised of thyroxine (T4), often known as the transport form of TH, and triiodothyronine (T3), the more bioactive form of TH. A TH spike occurs in the perinatal period of humans, and when disrupted, this spike can cause severe developmental defects. An analogous, but perhaps more overt, TH spike occurs in amphibians. TH is the sole hormone that drives amphibian metamorphosis, thus providing an excellent model for TH action. Our lab has previously developed the cultured tailfin (C-fin) assay, which uses biopsies from premetamorphic Rana catesbeiana tadpole tailfins cultured in the presence of an exogenous chemical of concern to assess perturbations to TH- and stress-responsive gene transcript levels by QPCR.
This thesis uses the C-fin assay to assess the efficacy of removal of biological TH- and stress-altering activity in conventional municipal wastewater treatment systems. We first assess the successive levels of a full-scale conventional activated sludge (CAS) MWWTP in its ability to reduce perturbations of mRNA transcript levels of the critical TH receptors alpha (thra) and beta (thrb), and stress responsive gene transcripts superoxide dismutase (sod), catalase (cat) and heat shock protein 30 (hsp30). Secondary treatment of wastewater effluents removes cellular stress perturbations when compared to influents, but thr disruptions remain after conventional secondary wastewater treatment. We then assess three pilot-sized conventional secondary MWWTP configurations run at two operational conditions. The C-fin assay results suggest that the current understanding of operational conditions and the efficiency of complex MWWTP configurations is not clear-cut when assessed by biological endpoints such as the transcript abundance perturbations in the C-fin assay.
Finally, the C-fin assay is used to investigate transcript profiles of genes of interest when the tissues are treated with the endogenous hormones T3, T4, and estradiol (E2). Our results indicate that T4 acts as more than solely a T3-prohormone and that gene expression levels in response to the two different forms of TH can be T3 or T4 specific. E2 effects, although implicated in altering TH-mediated responses in other contexts, do not affect TH-responsive gene transcripts in the C-fin. The data presented use the novel C-fin assay to challenge and advance the currently accepted views of TH-action, as well as develop necessary yet practical biological knowledge for management of emerging contaminant release from MWWTPs. / Graduate / 0383 / 0768 / 0307 / polaw@uvic.ca
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Stress and metabolic responses to municipal wastewater effluent exposure in rainbow trout effluentIngs, Jennifer Sophia January 2011 (has links)
Municipal wastewater effluent (MWWE) is an important source of pollution in the aquatic environment impacting fish. MWWE is a complex mixture of chemicals including pharmaceuticals, personal care products, industrial chemicals and pesticides. A link between reproductive endocrine disruption and MWWE exposure has been established in fish, but less is known about the effects of MWWE on non-reproductive endocrine disruption. The overall objective of this thesis was to examine the impacts of MWWE exposure on the stress response and intermediary metabolism in rainbow trout (Oncorhynchus mykiss). In fish, the primary adaptive organismal stress response involves the activation of hypothalamic-sympathetic-chromaffin axis to produce catecholamines, predominantly epinephrine, and the hypothalamic-pituitary-interrenal (HPI) axis to produce cortisol. Both of these hormones play a key role in elevating plasma glucose levels that is essential to fuel the increased energy demand associated with stress. Along with the organismal stress response, the cellular stress response, involving the synthesis of a suite of heat shock proteins (hsps), also plays an important role in protecting cellular protein homeostasis in response to stressors, including toxicants.
The impact of MWWE on stress-related pathways were identified using a low-density trout cDNA microarray enriched with genes encoding for proteins involved in endocrine-, stress- and metabolism-related processes. This was further confirmed by assessing plasma hormone and metabolite levels and stress-related targeted genes and proteins expression and enzyme activities in select tissues in rainbow trout. Studies were carried out in controlled field (caging) and laboratory experiments to examine the impacts of MWWE on stress and tissue-specific metabolic responses in rainbow trout. Further in vitro studies using rainbow trout hepatocytes in primary cultures were carried out to investigate the mechanism of action of two pharmaceuticals, atenolol and venlafaxine, found in relatively high concentrations in MWWE in impacting the stress-mediated glucose response.
In caged fish, MWWE exposure significantly elevated plasma cortisol and glucose concentrations, and altered the mRNA abundance of a number of stress-related genes, hormone receptors, glucose transporter 2 and genes related to immune function. When fish were exposed to an acute handling stress following a 14 d exposure to MWWE, the cortisol response was abolished and the glucose response was attenuated. The effects on cortisol did not correlate with changes in the expression of genes involved in cortisol biosynthesis, but were associated with an increase in hepatic glucocorticoid receptor (GR) protein expression.
Upon further investigation in controlled laboratory studies, MWWE exposure elevated constitutive hsp 70 and hsp90 expression after 8 d exposure, which correlated with a decrease in glycogen levels in the liver in fish exposed to a high concentration of MWWE compared to control fish, pointing to a MWWE-induced increase in liver energy demand. By 14 d, glycogen stores were replenished, and this was commensurate with increases in liver gluconeogenic capacity, including increases in the activities of phosphoenolpyruvate carboxykinase (PEPCK) and alanine aminotransferase (AlaAT), along with a decrease in liver GR expression. In the heart, GR protein expression increased in treated fish, and the activity of pyruvate kinase increased, indicating an increase in glycolytic capacity. Subjecting the MWWE exposed fish to a secondary handling disturbance (acute stress) led to an attenuated plasma cortisol and glucose response compared to the control group. This corresponded with a reduced liver gluconeogenic capacity and a lower liver and heart glycolytic capacities, reflecting a disturbance in the energy substrate repartitioning that is essential to cope with stress.
While it is difficult to establish causative agents from a complex mixture such as MWWE, the two pharmaceutical that were tested impacted glucose production. Specifically, atenolol and venlafaxine disrupted the epinephrine-induced glucose production, but did not modify cortisol-mediated glucose production in trout hepatocytes. The suppression of epinephrine-mediated glucose production by atenolol and venlafaxine was abolished by cAMP analogue (8-bromo cAMP) or glucagon (a metabolic hormone that increases glucose production). This suggests that both drugs disrupt β-adrenoceptor signaling, while it remains to be determined if the response is receptor isoform-specific.
Altogether MWWE exposure disrupts the organismal and cellular stress responses in trout. Key targets for MWWE impact leading to the impaired cortisol and metabolic responses to stress include liver and heart GR expression, liver gluconeogenic capacity, and liver, heart and gill glycolytic capacities. Most significantly, MWWE impairs the ability to metabolically adjust to a secondary acute stressor, which is an important adaptive process that is integral to successful stress performance. From an environmental stand-point, long-term exposure to MWWE will lead to reduced fitness and will compromise the capacity of fish to cope with additional stressor, including escape from predators.
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