Sexual reproduction is an important mechanism shaping plant community composition that will likely be affected by unprecedented rates of climate change in Canada’s North. To anticipate potential changes in plant communities, I aim to understand how changing environmental conditions affect the processes of seed production and seedling emergence, and determine the overall impacts on the reproductive potential of alpine tundra vegetation in Yukon, Canada. I tested the effect of soil warming and nitrogen addition treatments on the timing and success of sexual reproduction of the six tundra species; Dryas octopetala M. Vahl, Salix arctica Pall, Salix reticulata L., Lupinus arcticus L., Carex microchaeta Holm, and Hierochloë alpina (Sw.) R. & S. A summer snow event occurred on 2 July 2012, and I considered the impacts of such an event on the reproductive timing and success of the study species. I also examined the influence of seed availability and soil conditions on initial seedling emergence of three tundra species and three boreal species. I applied seed to natural disturbance sites with bare substrate exposed, and to plots with altered soil temperature and nitrogen availability. Results indicated that reproductive phenology, seed production, and seed viability of tundra species were not affected by increases in soil temperature and/or nitrogen availability but were impacted by the snowfall event. In addition, changes in soil temperature and nitrogen did not affect seedling emergence. Seedling emergence of both boreal and tundra species increased on bare substrates, indicating that surface disturbance creates opportunities for seedling establishment. Overall, my study shows that factors affecting seed production and local disturbance will have greater impact on the success of sexual reproduction in tundra plant communities than changes in soil temperature or nutrients caused by climate change.

No description available.

Zugl.: Diss. ETH Zürich, 1980.

Thesis (Ph. D.)--University of Washington, 2002. / Vita. Includes bibliographical references (leaves 100-117).

The acid phosphomonoesterase activity associated with two major rooting strategies in arctic tundra plants was examined: that of Eriophorum vagina tum, a dominant plant in tussock tundra ecosystems, with its predominantly non-mycorrhizal root system; and that of ectomycorrhizal roots. Eriophorum has phosphatase activity which is evenly distributed along its root surface, has a pH optimum at soil pH (3.5-4.0), and continues at substantial rates at 1 °C. Inorganic phosphorus inhibits activity only 7 to 19%. In addition, Eriophorum has phosphatase activity associated with all the "below-ground" components of its tussock growth form: dead roots, leaf sheaths, and soil. Plants with higher tissue phosphorus growing in soils with higher available phosphate in general had higher live and dead root, leaf sheath, and soil phosphatase activity in both natural and manipulated sites of higher plant productivity. Yearly and seasonal variation sometimes exceeded differences among treatments, suggesting that enzyme activity would not provide a reliable measure of plant or soil phosphorus levels. Experiments with radiolabeled inositol hexaphosphate showed that Eriophorum is able to hydrolyze and absorb inorganic phosphate from an organic phosphate source. A comparison of enzyme hydrolysis rates with inorganic phosphate assimilation rates indicates that organic phosphate hydrolysis may occur as rapidly as inorganic phosphate absorption. Inorganic phosphate released by root surface phosphatase activity could satisfy approximately 65% of the annual phosphate demand of Eriophorum. Phosphatases of two ectomycorrhizal fungi (Cenococcum geophilum and Entoloma sericeum) responded similarly to growth in axenic culture at 2 or 50 micromolar KH₂PO₄ or sodium inositol hexaphosphate: surface Vmax estimates were significantly greater for 2 micromolar- than for 50 micromolar-grown isolates. The presence of constitutive extracellular soluble phosphatase activity resulted in the appearance of inorganic phosphate in media initially supplied only with organic phosphate. The surface acid phosphatase activity of field-collected ectomycorrhizal roots of arctic Salix and Betula, however, did not respond in a consistent way to differences in soil characteristics. Activity differed more among "color types" or fungal types than among sites of different soil characteristics. / Ph. D.

Rapid climate change is driving increases in tundra vegetation productivity and altering the frequency and severity of natural disturbances across the Arctic. While tundra vegetation change has been widespread, there is still uncertainty about the influence of fine-scale factors on change and the role of interactions between warming, disturbance, and vegetation change. In my MSc research I investigated how Arctic tundra vegetation is responding to ongoing climate change and more severe tundra fire in the western Canadian Arctic. In the first part of my thesis I measured post-fire soil and vegetation recovery along a burn severity gradient at six fires, which burned in 2012 in the Northwest Territories. My observations suggest that deciduous shrub communities (dominated by Betula glandulosa) are resilient to high severity fire and that severe fire promotes edaphic conditions that favor the persistence of this vegetation type. In the second part of my thesis, I investigated the spatial patterns of trends in tundra vegetation productivity over the past three decades using Random Forests machine learning to analyze Enhanced Vegetation Index (EVI) data derived from Landsat imagery. My Random Forests models of the relationship between Landsat EVI trends and biophysical variables showed that two-thirds of the western Canadian Arctic productivity has increased during the past three decades and that this change is occurring most rapidly in dwarf and upright shrub-dominated regions. Taken together, my research demonstrates that shrub tundra communities are well adapted to severe fire and show increasing productivity in response to warming Arctic temperature. My research also indicates that these relationships can be highly complex at finer scales, where they are mediated by local variations in microclimate, topography, and moisture. / Graduate

Over the past millennia, complex terrestrial ecosystems have evolved in the Arctic. However, the stability of these unique ecosystems is in jeopardy because of climate changes. Due to the fact that Arctic soils store great amounts of carbon (C) in soil organic matter (SOM), any change that may occur in SOM with climate changes may substantially affect many aspects of Arctic ecosystems such as vegetation, animals, and humans. On a more global perspective, any change in Arctic SOM has the potential of modifying the overall world climate by affecting the global greenhouse gas (GHG) budget. A better understanding of the soil factors that affect soil N and C cycling at the landscape scale, such as moisture, temperature, and SOM characteristics, is necessary to produce better models. The overall objective of this study was to characterize the properties of SOM in Arctic soils and their influence on soil N and C cycling dynamics � including GHG emissions � at the landscape scale. This study was conducted in three distinct Arctic ecosystems: Sub-Arctic (Churchill, MB), Low-Arctic (Daring Lake, NWT), and High-Arctic (Truelove, NU). For each site, the sampling locations were evenly divided into five landform units: 1) upper slope (Up), 2) back slope (Back), and 3) lower slope (Low) for catena sites, and 4) hummock (Hum) and 5) wedges of hummock (W) for hummocky sites (i.e., hummock in Churchill and ice-wedge polygons in Truelove). All sites were sampled at the end of their growing season (from 2 to 3 weeks before plant senescence). The characteristics of SOM were assessed using three methods: 1) density fractionation to separate the uncomplexed light fraction (LF) from heavy fraction (HF) of SOM (LF < 1.55 g mL-1 < HF), 2) solid-state CPMAS 13C nuclear magnetic resonance (NMR) spectroscopy that determined the relative proportions of carbonyl-C (CbyC), alkyl-C (AC), aromatic-C (AroC), o-alkyl-C (OAC), and carbohydrates-C (CC), and 3) water-extractable organic matter (WEOM) that estimated SOM diluted in soil solution. Soil gross N mineralization was measured in situ using 15N dilution technique. Soil GHG emissions [nitrous oxide (N2O), methane (CH4), and carbon dioxide (CO2)] were measured in situ using a multicomponent Fourier transform infrared gas analyzer coupled with an automated dark chamber. The first study showed that organic surface soils, which had more than 17% soil organic C (SOC) by weight, contained relatively more labile SOM than mineral surface soils (< 17% SOC). For example, OAC:AroC ratios of the organic soils ranged from 25 to 75% greater compared to mineral soils. At Churchill, Daring Lake, and Truelove, 53, 73, and 20% of the C and N was included in the LF, respectively. All results show that the organic soils of Sub- and Low-Arctic ecosystems sampled for this study contain more fresh and un-decomposed plant residues than High-Arctic organic soils. The second study showed that both topography and ecosystems had a significant impact on gross N mineralization and CO2 emission rates. For example, at Churchill, gross N mineralization increased about 6-fold from upper slope to lower slope areas. Similarly, at Daring Lake, CO2 emissions increase about 5-fold from upper slope to lower slope areas. Topography and ecosystems had a very limited impact on soil N2O and CH4 emissions most likely because net emissions were extremely low. The third study showed that soil moisture, SOM quantity, and labile SOM parameters such as OAC:AroC and water-soluble organic carbon (WSOC) positively influenced gross N mineralization, N2O, and CO2 emissions, whereas the relative proportion of AroC negatively influenced gross N mineralization, N2O, and CO2 emissions. Relationships between SOM characteristics and CH4 emissions were not significant. This study showed that Up and Back areas tended to store relatively more recalcitrant SOM (AroC) than Low, Hum, and W areas, suggesting less fresh plant input on these landform units. Assessing SOM qualities with the ability of the soils to mineralize N (i.e., gross N mineralization) and release GHG at the landscape scale and across the Arctic represents a great advance in the understanding of these complex and unique ecosystems. Lower proportion of fresh and labile SOM found on Up and some Back landform units compared to Low and hummocky sites suggest that plants have more difficulties establishing and growing on these landform units (e.g., Up and Back) that experience harsh climates. Therefore, generalizations of the climate change impacts on soil N and C cycling processes throughout Arctic landscapes and ecosystems are less certain if topography is not considered. These results are particularly important because they can be used to produce better models that evaluate SOM stocks and dynamics under several climate scenarios and across Arctic landscapes and ecosystems.

Over the past millennia, complex terrestrial ecosystems have evolved in the Arctic. However, the stability of these unique ecosystems is in jeopardy because of climate changes. Due to the fact that Arctic soils store great amounts of carbon (C) in soil organic matter (SOM), any change that may occur in SOM with climate changes may substantially affect many aspects of Arctic ecosystems such as vegetation, animals, and humans. On a more global perspective, any change in Arctic SOM has the potential of modifying the overall world climate by affecting the global greenhouse gas (GHG) budget. A better understanding of the soil factors that affect soil N and C cycling at the landscape scale, such as moisture, temperature, and SOM characteristics, is necessary to produce better models. The overall objective of this study was to characterize the properties of SOM in Arctic soils and their influence on soil N and C cycling dynamics � including GHG emissions � at the landscape scale. This study was conducted in three distinct Arctic ecosystems: Sub-Arctic (Churchill, MB), Low-Arctic (Daring Lake, NWT), and High-Arctic (Truelove, NU). For each site, the sampling locations were evenly divided into five landform units: 1) upper slope (Up), 2) back slope (Back), and 3) lower slope (Low) for catena sites, and 4) hummock (Hum) and 5) wedges of hummock (W) for hummocky sites (i.e., hummock in Churchill and ice-wedge polygons in Truelove). All sites were sampled at the end of their growing season (from 2 to 3 weeks before plant senescence). The characteristics of SOM were assessed using three methods: 1) density fractionation to separate the uncomplexed light fraction (LF) from heavy fraction (HF) of SOM (LF < 1.55 g mL-1 < HF), 2) solid-state CPMAS 13C nuclear magnetic resonance (NMR) spectroscopy that determined the relative proportions of carbonyl-C (CbyC), alkyl-C (AC), aromatic-C (AroC), o-alkyl-C (OAC), and carbohydrates-C (CC), and 3) water-extractable organic matter (WEOM) that estimated SOM diluted in soil solution. Soil gross N mineralization was measured in situ using 15N dilution technique. Soil GHG emissions [nitrous oxide (N2O), methane (CH4), and carbon dioxide (CO2)] were measured in situ using a multicomponent Fourier transform infrared gas analyzer coupled with an automated dark chamber. The first study showed that organic surface soils, which had more than 17% soil organic C (SOC) by weight, contained relatively more labile SOM than mineral surface soils (< 17% SOC). For example, OAC:AroC ratios of the organic soils ranged from 25 to 75% greater compared to mineral soils. At Churchill, Daring Lake, and Truelove, 53, 73, and 20% of the C and N was included in the LF, respectively. All results show that the organic soils of Sub- and Low-Arctic ecosystems sampled for this study contain more fresh and un-decomposed plant residues than High-Arctic organic soils. The second study showed that both topography and ecosystems had a significant impact on gross N mineralization and CO2 emission rates. For example, at Churchill, gross N mineralization increased about 6-fold from upper slope to lower slope areas. Similarly, at Daring Lake, CO2 emissions increase about 5-fold from upper slope to lower slope areas. Topography and ecosystems had a very limited impact on soil N2O and CH4 emissions most likely because net emissions were extremely low. The third study showed that soil moisture, SOM quantity, and labile SOM parameters such as OAC:AroC and water-soluble organic carbon (WSOC) positively influenced gross N mineralization, N2O, and CO2 emissions, whereas the relative proportion of AroC negatively influenced gross N mineralization, N2O, and CO2 emissions. Relationships between SOM characteristics and CH4 emissions were not significant. This study showed that Up and Back areas tended to store relatively more recalcitrant SOM (AroC) than Low, Hum, and W areas, suggesting less fresh plant input on these landform units. Assessing SOM qualities with the ability of the soils to mineralize N (i.e., gross N mineralization) and release GHG at the landscape scale and across the Arctic represents a great advance in the understanding of these complex and unique ecosystems. Lower proportion of fresh and labile SOM found on Up and some Back landform units compared to Low and hummocky sites suggest that plants have more difficulties establishing and growing on these landform units (e.g., Up and Back) that experience harsh climates. Therefore, generalizations of the climate change impacts on soil N and C cycling processes throughout Arctic landscapes and ecosystems are less certain if topography is not considered. These results are particularly important because they can be used to produce better models that evaluate SOM stocks and dynamics under several climate scenarios and across Arctic landscapes and ecosystems.

Since humans began to permanently settle locations for extended periods of time there has been the challenge to safely dispose of, or treat human effluent. In specific to the communities of Nunavut and Arctic Canada, the treatment of wastewater has been particularly challenging. The harsh climate, remote nature and socio-economic factors are a few of the aspects which make the treatment of wastewater problematic in Canadian Arctic communities. In the past several decades a number of conventional and alternative wastewater treatment systems (e.g. lagoons and tundra wetlands) have been proposed and implemented in Nunavut and other remote Arctic communities. Knowledge of performance of these systems is limited, as little research has been conducted and regulatory monitoring has been poorly documented or not observed at all. Also, in the past, the rational design process of treatment systems in Arctic communities has not acknowledged cultural and socio-economic aspects, which are important for the long-term management and performance of the treatment facilities in Arctic communities. From 2008 to 2010 I characterized and studied the performance of several tundra wastewater treatment wetlands in the Kivalliq Region of Nunavut, as well as two in the Inuvaliat Region of the Northwest Territories. Performance testing occurred weekly throughout the summer of 2008. Characterization included surveys of plant communities in the tundra wetlands, specifically analyzing the relationship between Carex aquatilis and various nutrient contaminants in wastewater. Through their characterization I was able to provide greater insight into primary treatment zones within the wetland, and identify the main potential mechanisms for the treatment wastewater in the Arctic. I also studied the performance of a horizontal subsurface flow (HSSF) constructed wetland in Baker Lake Nunavut; the first system of its kind in the Canadian Arctic. The weekly performance study showed average weekly percent reduction in all parameters, with small deviations immediately after snow-melt and at the beginning of freeze-up. For the six parameters monitored I observed reductions of 47-94% cBOD5, 57-96% COD, 39-98% TSS, >99% TC, >99% E. coli, 84-99% NH3-Nand 80-99% TP for the six tundra treatment wetlands. Whereas, the wetland characterization study through the use of spatial interpolations on each of the wetlands and their water quality showed that concentrations of the wastewater parameters decreased the most in the first 100m of the wetland in all three treatment wetlands used in this portion of the analysis (Chesterfield Inlet, Paulatuk and Ulukhaktok). Areas of greatest concentration where shown to follow preferential flow paths with concentrations decreasing in a latitudinal and longitudinal direction away from the wastewater source. The Paulatuk and Ulukhaktok treatment wetlands were observed to effectively polish pre-treated wastewater from the facultative lake and engineered lagoon, with removals of key wastewater constituents of cBOD5, TSS and NH3-N to near background concentrations. And despite the absence of pre-treatment in Chesterfield Inlet, the wetland was also observed to effectively treat wastewater to near background concentrations. Further characterization on the composition of the sedge C. aquatilis, showed a high percent cover of the species corresponded with areas of high concentration of NH3-N in the wastewater. A principal components analysis verified the spatial results showing correlation between C. aquatilis cover and NH3-N concentrations. Analysis also showed strong positive relationship between sites closer to the source of wastewater and C. aquatilis. No correlation was found between the other parameters analyzed and C. aquatilis. The first year of study of the HSSF constructed wetland showed promising mean removals in cBOD5, COD, TSS, E. coli, Total Coliforms, and TP throughout the summer of 2009; removals of 25%, 31%, 52%, 99.3%, 99.3%, and 5% were observed respectively. However, the second year of study in 2010 the system did not perform as expected, and concentrations of effluent actually increased. I concluded that a high organic loading during the first year of study saturated the system with organics. Finally, a review of planning process and regulatory measures for wastewater in Arctic communities and the impending municipal wastewater standards effluent resulted in the following recommendations; i) wastewater effluent standards should reflect the diverse arctic climate, and socio-economic environment of the northern communities, ii) effluent standards should be region or even community specific in the Arctic, and iii) for planning and management of wastewater incorporation of Inuit understanding of planning and consultation needs to be incorporated in the future. This research has several major implications for wastewater treatment and planning for Nunavut and other Arctic Regions. The performance and characterization of tundra treatment wetlands fills significant gaps in our understanding of their performance and potential mechanisms of treatment, and treatment period in the Kivalliq Region. Although the HSSF constructed wetland failed, further research into engineered/augmented treatment wetlands should be considered as they provide low-cost low maintenance solutions for remote communities. Finally, the data collected in this study will provide significant insight into the development of new municipal wastewater effluent standards for northern communities, which will be reflected in the Fisheries Act.

Seasonal patterns of snow accumulation in the Northern Hemisphere are changing in response to variations in Arctic climate. These changes have the potential to influence global climate, regional hydrology, and sensitive ecosystems as they become more pronounced. To refine our understanding of the role of snow in the Earth system, improved methods to characterize global changes in snow extent and mass are needed. Current space-borne observations and ground-based measurement networks lack the spatial resolution to characterize changes in volumetric snow properties at the scale of ground observed variation. Recently, radar has emerged as a potential complement to existing observation methods with demonstrated sensitivity to snow volume at high spatial resolutions (< 200 m). In 2009, this potential was recognized by the proposed European Space Agency Earth Explorer mission, the Cold Regions High Resolution Hydrology Observatory (CoReH2O); a satellite based dual frequency (17.2 and 9.6 GHz) radar for observation of cryospheric variables including snow water equivalent (SWE). Despite increasing international attention, snow-radar interactions specific to many snow cover types remain unevaluated at 17.2 or 9.6 GHz, including those common to the Canadian tundra. This thesis aimed to use field-based experimentation to close gaps in knowledge regarding snow-microwave interaction and to improve our understanding of how these interactions could be exploited to retrieve snow properties in tundra environments. Between September 2009 and March 2011, a pair of multi-objective field campaigns were conducted in Churchill, Manitoba, Canada to collect snow, ice, and radar measurements in a number of unique sub-arctic environments. Three distinct experiments were undertaken to characterize and evaluate snow-radar response using novel seasonal, spatial, and destructive sampling methods in previously untested terrestrial tundra environments. Common to each experiment was the deployment of a sled-mounted dual-frequency (17.2 and 9.6 GHz) scatterometer system known as UW-Scat. This adaptable ground-based radar system was used to collect backscatter measurements across a range of representative tundra snow conditions at remote terrestrial sites. The assembled set of measurements provide an extensive database from which to evaluate the influence of seasonal processes of snow accumulation and metamorphosis on radar response. Several advancements to our understanding of snow-radar interaction were made in this thesis. First, proof-of-concept experiments were used to establish seasonal and spatial observation protocols for ground-based evaluation. These initial experiments identified the presence of frequency dependent sensitivity to evolving snow properties in terrestrial environments. Expanding upon the preliminary experiments, a seasonal observation protocol was used to demonstrate for the first time Ku-band and X-band sensitivity to evolving snow properties at a coastal tundra observation site. Over a 5 month period, 13 discrete scatterometer observations were collected at an undisturbed snow target where Ku-band measurements were shown to hold strong sensitivity to increasing snow depth and water equivalent. Analysis of longer wavelength X-band measurements was complicated by soil response not easily separable from the target snow signal. Definitive evidence of snow volume scattering was shown by removing the snowpack from the field of view which resulted in a significant reduction in backscatter at both frequencies. An additional set of distributed snow covered tundra targets were evaluated to increase knowledge of spatiotemporal Ku-band interactions. In this experiment strong sensitivities to increasing depth and SWE were again demonstrated. To further evaluate the influence of tundra snow variability, detailed characterization of snow stratigraphy was completed within the sensor field of view and compared against collocated backscatter response. These experiments demonstrated Ku-band sensitivity to changes in tundra snow properties observed over short distances. A contrasting homogeneous snowpack showed a reduction in variation of the radar signal in comparison to a highly variable open tundra site. Overall, the results of this thesis support the single frequency Ku-band (17.2 GHz) retrieval of shallow tundra snow properties and encourage further study of X-band interactions to aid in decomposition of the desired snow volume signal.