Thesis--Virginia Polytechnic Institute and State University. / Vita. Abstract. Includes bibliographical references (leaves 51-70). Also available via the Internet.

Thesis--Virginia Polytechnic Institute and State University. / Also available via the Internet.

A biological and integrated control program for Carduus thistles was developed using the biological control agents Rhinocyllus conicus Froelich and Ceuthorhynchidius horridus (Panzer) and the herbicide 2,4-Dichlorophenoxyacetic acid (2,4-D). Field studies on the development of Ceuthorhynchidius horridus (Panzer) on Carduus thistles in Virginia between 1975-1978 showed that the weevil has one generation annually. Oviposition occurred from mid-December until early April, and larvae occurred in rosettes from late December through late May. Teneral adults, which appeared in mid-May through June, underwent an aestival diapause during most of July through September. Adult reappearance in late September coincided with an increase in feeding. Although adult feeding marks, teneral adults and first and third instar larvae were easily found in the field, detection of eggs, second instars or overwintering adults was difficult and time consuming. Acute and chronic effects of spring application of 2,4-D (LVA) on adult C. horridus were examined. LC₅₀ values for males (70.2 kg/ha) and females (61.4 kg/ha) corresponded to 41.7 and 36.6 times, respectively, the recommended application rate of 1.68 kg/ha. Treatment with 1.68 kg/ha did not affect adult survival, but increased dosages (16.8-147.8 kg/ha) caused significantly greater mortality. Adult vitality, measured by number of feeding marks/weevil and weight change/time, was unaffected by the herbicide. Field application of herbicides did not prevent survival, reproduction, or population increase of C. horridus. Herbicidal effect on larval R. conicus was studied by examining the mortality, emergence rates and weights of weevils developing from plants treated with 2,4-D (LVA). Infested heads, obtained by caging ovipositing R. conicus on primary heads of musk thistle (Carduus nutans L.) (resembles C. thoermeri Weinmann), were treated with 2,4-D at 1.68 kg/ha 0-3 weeks after oviposition. Blooms treated immediately following oviposition failed to support larval development beyond the second instar. Developmental times and weights of weevils that emerged from blooms sprayed at 1, 2, and 3 weeks were not significantly different from controls. Plants sprayed up to 2 weeks after oviposition (late-bud to early-bloom) did not produce viable seeds. Treatments at 3 weeks after oviposition (full-bloom) allowed 10% germination of seeds not damaged by R. conicus in primary heads, and plants survived to produce additional heads. Acute and chronic effects of the herbicide 2,4-D on adult R. conicus were also examined. LC₅₀ values for adults prior to over-wintering (males 78.6 kg/ha; females 61.0 kg/ha) were lower than those for overwintered weevils (males 117.1 kg/ha; females 126.6 kg/ha), but were still at least 40 times the recommended application rate of 1.68 kg/ha. Survival was not significantly affected by direct application of 2,4-D at 1.68 kg/ha plus sticker or by exposure to herbicide sprays and residue while on musk thistle rosettes. Mean egg production/ovipositing female/3 day period was not significantly different (range = 5.44 - 7.60), regardless of the 2,4-D dosage applied (range = 0.0 - 147.84 kg/ha); all ovipositing weevils produced viable eggs. Field treatment with up to 2.24 kg/ha of 2,4-D resulted in death of host plants, but did not prevent survival or reproduction of R. conicus populations. / Ph. D.

Musk thistle (Carduus nutans) seed production from early developing (terminal and first lateral) heads was reduced by 10% in 1973 and 75% in 1974 due to larval feeding of Rhinocyllus conicus. This was caused by heavy infestation of the early heads (45% in 1973, 70% in 1974) with a subsequent 5-fold increase in the number of weevil pupation chambers in these heads. Total seed production was reduced by 35-36% in both years despite increased plant vigor due to better growing conditions in 1974. Larval feeding reduced viability of mature musk thistle seeds. Plumeless thistle (C. acanthoides) seed production from early heads decreased only 5% in 1973 and 4% in 1974 due to low rates of infestation (9% in 1973, 5% in 1974). Total seed production was reduced 0.2% in 1973 and 0.1% in 1974. Weevil larvae developing in plumeless thistle heads were more heavily parasitized than those in musk thistles. Parasitoids suffered high mortality due to entrapment in weevil pupation chambers (Aliolus curculionis, 837; Bracon mellitor, 10%), and did not inhibit weevil population buildup. These combined factors resulted in successful control of musk thistle at 1 Virginia site. R. conicus showed ovipositional preference for C. nutans over C. acanthoides heads both in the laboratory and under field conditions. Oviposition of an established weevil colony was better synchronized with musk than with plumeless thistles at a site where both thistles were present. Sequentially released, overwintered R. conicus were better synchronized with Carduus thistle development because of an extended ovipositional period, but the weevils suffered reduced fecundity. Ovipositional preference was for the earlier stages of musk thistle heads. Intermediate stages of plumeless thistle heads were preferred, and larval survival was reduced because of insufficient time for weevil development and subsequent depletion of food supply. Musk thistles subjected to mechanical and R. conicus-induced damage to terminal heads were shorter than control plants and suffered reduced seed production from terminal heads. Total head and seed production per thistle were not significantly reduced. Emerging adult weevils decreased in weight relative to crowding; no difference was detected between sexes (female, 13.4mg; male, 13.8mg). / Ph. D.