Made available in DSpace on 2014-06-11T19:27:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-06-06Bitstream added on 2014-06-13T20:16:21Z : No. of bitstreams: 1 buchala_fg_me_jabo.pdf: 194310 bytes, checksum: c5ffe9e9aa8cde854e11b73d2a8b4c74 (MD5) / Esta pesquisa objetivou demonstrar a ocorrência de reações sorológicas para Salmonella sp, Mycoplasma gallisepticum e Mycoplasma synoviae nas populações de aves de explorações não tecnificadas e com finalidade de subsistência, em propriedades rurais localizadas em áreas geográficas próximas a granjas de reprodutoras (matrizeiros) do Estado de São Paulo. Foram selecionadas três granjas de matrizes, oficialmente reconhecidas como sendo livres de S. Pullorum (SP), S. Gallinarum (SG), S. Enteritidis (SE), S. Typhimurium (ST), M. gallisepticum (MG) e M. synoviae (MS), e quinze criações vizinhas, onde a população de aves existente foi amostrada, com colheita e processamento de 406 soros sanguíneos. O diagnóstico foi realizado pela técnica de soroaglutinação rápida em placa. As frequências encontradas foram 73%, 73% e 100% de propriedades com aves sororreagentes aos antígenos testados de SP (teste da pulorose), MG e MS, respectivamente. As ocorrências observadas de aves sororreagentes foram de 16,5%, 30,3% e 40,6% para os antígenos de SP, MG e MS, respectivamente. Os dados obtidos indicam que os agentes estudados estão amplamente difundidos nas criações informais de aves de fundo de quintal, colocando em risco constante os criatórios de exploração industrial, os quais necessitam adotar e manter boas práticas de biosseguridade para preservar a integridade sanitária dos plantéis. / This investigation aimed to demonstrate the occurrence of serological reactions to Salmonella spp., Mycoplasma gallisepticum and Mycoplasma synoviae in backyard domestic poultry for own consume located next to parent flocks in the State of São Paulo. Three parent flocks officially recognised as free from S. Pullorum (SP), S. Gallinarum (SG), S. Enteritidis (SE), S. Typhimurium (ST), M. gallisepticum (MG) and M. synoviae (MS) and 15 neighbour backyard flocks were selected. A sample of 406 chickens from the backyard flocks was bled, and the diagnostic was carried out by the plate agglutination test. The frequencies found were 73%, 73% and 100% of flocks with chickens reacting to the antigens of SP, MG and MS, respectively. The frequencies of reacting chickens were 16.5%, 30.3% and 40.6% for the antigens of SP, MG and MS, respectively. The results show that the aetiological agents studied are widespread among the backyard flocks, posing a constant risk for the commercial poultry flocks, that need to adopt and keep good biosecurity practices to preserve their sanitary status.

Orientador: Luís Antonio Mathias / Banca: Angelo Berchieri Junior / Banca: Masaio Mizuno Ishizuka / Resumo: Esta pesquisa objetivou demonstrar a ocorrência de reações sorológicas para Salmonella sp, Mycoplasma gallisepticum e Mycoplasma synoviae nas populações de aves de explorações não tecnificadas e com finalidade de subsistência, em propriedades rurais localizadas em áreas geográficas próximas a granjas de reprodutoras (matrizeiros) do Estado de São Paulo. Foram selecionadas três granjas de matrizes, oficialmente reconhecidas como sendo livres de S. Pullorum (SP), S. Gallinarum (SG), S. Enteritidis (SE), S. Typhimurium (ST), M. gallisepticum (MG) e M. synoviae (MS), e quinze criações vizinhas, onde a população de aves existente foi amostrada, com colheita e processamento de 406 soros sanguíneos. O diagnóstico foi realizado pela técnica de soroaglutinação rápida em placa. As frequências encontradas foram 73%, 73% e 100% de propriedades com aves sororreagentes aos antígenos testados de SP (teste da pulorose), MG e MS, respectivamente. As ocorrências observadas de aves sororreagentes foram de 16,5%, 30,3% e 40,6% para os antígenos de SP, MG e MS, respectivamente. Os dados obtidos indicam que os agentes estudados estão amplamente difundidos nas criações informais de aves de "fundo de quintal", colocando em risco constante os criatórios de exploração industrial, os quais necessitam adotar e manter boas práticas de biosseguridade para preservar a integridade sanitária dos plantéis. / Abstract: This investigation aimed to demonstrate the occurrence of serological reactions to Salmonella spp., Mycoplasma gallisepticum and Mycoplasma synoviae in backyard domestic poultry for own consume located next to parent flocks in the State of São Paulo. Three parent flocks officially recognised as free from S. Pullorum (SP), S. Gallinarum (SG), S. Enteritidis (SE), S. Typhimurium (ST), M. gallisepticum (MG) and M. synoviae (MS) and 15 neighbour backyard flocks were selected. A sample of 406 chickens from the backyard flocks was bled, and the diagnostic was carried out by the plate agglutination test. The frequencies found were 73%, 73% and 100% of flocks with chickens reacting to the antigens of SP, MG and MS, respectively. The frequencies of reacting chickens were 16.5%, 30.3% and 40.6% for the antigens of SP, MG and MS, respectively. The results show that the aetiological agents studied are widespread among the backyard flocks, posing a constant risk for the commercial poultry flocks, that need to adopt and keep good biosecurity practices to preserve their sanitary status. / Mestre

No description available.

Thesis (Ph. D.)--University of Sydney, 2004. / Title from title screen (viewed 8 May 2008). A reference list of published articles is provided in Appendices. Includes copies of seven published papers, co-authored by Fanrong Kong. Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Dept. of Medicine. Includes bibliographical references. Also available in print form.

Mycoplasmas conform most closely with the theoretical concept of 'minimum cells', existing as the smallest, free-living organisms capable of self-replication. They survive as parasites of plants, insects, animals or humans, with the most common human colonising species being Mycoplasma hominis. M. hominis has been characterised as a human pathogen responsible for a variety of infections, which pose a significant threat particularly to immunocompromised patients and neonates. However little has been elucidated about the cell physiology and molecular structure of this organism. Of interest to this study were the investigation of the heat shock response of M. hominis and the diagnostic assays used for its detection. The heat shock response is a ubiquitous physiological feature of all organisms and displays unprecedented conservation. This phenomenon is particularly evident in the 70 kDa family of heat shock proteins (hsp70) which exhibits a high degree of homology between different species. The hsp70 gene from M. hominis was cloned and preliminary partial sequencing indicated the similarity with other hsp70 homologs. The regulation of hsp70 expression at the transcriptional and translational levels was investigated. The level of hsp70 mRNA was found to increase correspondingly in response to heat shock, more visibly than the level of hsp70 protein. However imrnunochemical studies of the M. hominis hsp70 translation product demonstrated further the homology with other species. To facilitate rapid diagnosis of M. hominis infections, a PCR ELISA diagnostic assay was developed and optimised. The amplification of a conserved region of the M. hominis 16S rRNA gene was linked to subsequent hybridisation to an appropriate capture probe in a microtiter plate format. The sensitivity of the assay was comparable to other molecular assays although the PCR ELISA produces more rapid results and is less labour intensive. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 1998.