"November 2002" / Includes bibliographical references (leaves 128-153) / xviii, 153 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Soil and Water, 2002

Bibliography: leaves 138-160. The 152 species of mycorrhizal fungi can be difficult to identify and quantify because the taxonomy of these fungi is based on the description of spores, which is time consuming, requires considerable expertise and cannot be assumed to reflect the situation within the root. Few attempts have been made to identify the species which are present in roots. Several approaches have been identified in previous work and the development of sensitive molecular methods for identification and quantification of two species of arbuscular mycorrhizal (AM) fungi are described in this study. Mycorrhizal fungal communities were sampled in both natural and agricultural ecosystems at two sites in South Australia. The combination of spore identification from trap culture and field-collected soil promises to be an effective means to study diversity of AM fungi in a particular system. PCR primers for Glomus mosseae and Gigaspora margarita were designed from the internal transcribed spacer (ITS) sequences of field-collected spores, with the aim of providing tools for field diagnosis.

Bibliography: leaves 138-160. / xii, 160 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The 152 species of mycorrhizal fungi can be difficult to identify and quantify because the taxonomy of these fungi is based on the description of spores, which is time consuming, requires considerable expertise and cannot be assumed to reflect the situation within the root. Few attempts have been made to identify the species which are present in roots. Several approaches have been identified in previous work and the development of sensitive molecular methods for identification and quantification of two species of arbuscular mycorrhizal (AM) fungi are described in this study. Mycorrhizal fungal communities were sampled in both natural and agricultural ecosystems at two sites in South Australia. The combination of spore identification from trap culture and field-collected soil promises to be an effective means to study diversity of AM fungi in a particular system. PCR primers for Glomus mosseae and Gigaspora margarita were designed from the internal transcribed spacer (ITS) sequences of field-collected spores, with the aim of providing tools for field diagnosis. / Thesis (Ph.D.)--University of Adelaide, Dept. of Soil and Water, 2000?

Bibliography: leaves 138-160. / xii, 160 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The 152 species of mycorrhizal fungi can be difficult to identify and quantify because the taxonomy of these fungi is based on the description of spores, which is time consuming, requires considerable expertise and cannot be assumed to reflect the situation within the root. Few attempts have been made to identify the species which are present in roots. Several approaches have been identified in previous work and the development of sensitive molecular methods for identification and quantification of two species of arbuscular mycorrhizal (AM) fungi are described in this study. Mycorrhizal fungal communities were sampled in both natural and agricultural ecosystems at two sites in South Australia. The combination of spore identification from trap culture and field-collected soil promises to be an effective means to study diversity of AM fungi in a particular system. PCR primers for Glomus mosseae and Gigaspora margarita were designed from the internal transcribed spacer (ITS) sequences of field-collected spores, with the aim of providing tools for field diagnosis. / Thesis (Ph.D.)--University of Adelaide, Dept. of Soil and Water, 2000?