Abstract not available

Spontaneous term labour is associated with increased inflammatory events in the myometrium including cytokine production and leukocyte infiltration. We hypothesized that mechanical stretch of the uterine wall by the growing fetus facilitates peripheral leukocyte transendothelial migration into the term pregnant myometrium through the release of various cytokines. The current study demonstrated that static mechanical stretch directly induces secretion of multiple cytokines and chemokines by human myometrial smooth muscle cells. Stretch-induced cytokines (1) increased vascular permeability; (2) enhanced leukocyte adhesion to the endothelium of the surrounding uterine microvasculature by (3) inducing the expression of endothelial cell adhesion molecules; and (4) directed the transendothelial migration of peripheral neutrophils. Our data provide a direct proof of mechanical regulation in leukocyte recruitment from the uterine blood vessels, which represents a putative mechanism for the leukocyte infiltrate seen in the myometrium during labour and postpartum involution.

Spontaneous term labour is associated with increased inflammatory events in the myometrium including cytokine production and leukocyte infiltration. We hypothesized that mechanical stretch of the uterine wall by the growing fetus facilitates peripheral leukocyte transendothelial migration into the term pregnant myometrium through the release of various cytokines. The current study demonstrated that static mechanical stretch directly induces secretion of multiple cytokines and chemokines by human myometrial smooth muscle cells. Stretch-induced cytokines (1) increased vascular permeability; (2) enhanced leukocyte adhesion to the endothelium of the surrounding uterine microvasculature by (3) inducing the expression of endothelial cell adhesion molecules; and (4) directed the transendothelial migration of peripheral neutrophils. Our data provide a direct proof of mechanical regulation in leukocyte recruitment from the uterine blood vessels, which represents a putative mechanism for the leukocyte infiltrate seen in the myometrium during labour and postpartum involution.

Diss. (sammanfattning) Linköping : Univ., 2001. / Härtill 4 uppsatser.

Thesis (Ph. D.)--University of Nevada, Reno, 2008. / "August 2008." Includes bibliographical references. Online version available on the World Wide Web.

Thesis (MTech (Biomedical Technology))--Cape Technikon,1997. / The aims of this study were to measure: 1. Myometrial cells of menopausal uteri to establish whether they atrophy after the menopause. 2. Myometrial cells at different phases of the menstrual cycle to investigate the influences of oestrogen and progesterone during the cycle. 3. Myometrial cells in the fundus and lower uterine segment to establish whether they differ in size. 4. Myometrial cells of pregnant uteri to investigate the effect of the hormonal status of pregnant women on the size of myometrial cells. 5. Neoplastic cells of leiomyomas of the uterus to investigate whether these benign tumours behave in the same manner as myometrium or, because they are neoplastic, they react differently. A preliminary investigation was undertaken to establish the optimal methodology for this study to measure myometrial and leiomyoma nuclei in the uterus. The aims of this preliminary investigation were: 1. To test the reproducibility of measurements of myometrial and leiomyoma nuclei in transverse and cross section. 2. To test five histological staining methods to ascertain the best method for a morphometric study on uterine cells. 3. To find the minimum sample size of nuclei per section of myometrium or leiomyoma in order to yield statistically significant results. This preliminary study found that the Haematoxylin and Eosin stain gave the most statistically reproducible measurements. Subjective assessment of the five staining methods also found Haematoxylin and Eosin to be optimal. It was also found during the preliminary study that measuring the myometrial nuclei in cross rather than transverse section gave the most statistically reproducible measurements. It was also found that it was best to use an axial ratio criterion of 0,9 when measuring cross-sectioned myometrial nuclei. The optimum sample size per section was also investigated and it was found that measuring 100 nuclei was optimal. It was found that in the uteri used in this study there was no statistically significant decrease in nuclear size after the menopause. It was also found that there was no statistically significant difference in nuclear size during the different phases of the menstrual cycle. There was also no notable difference in nuclear size between nuclei in the fundus and lower segment of the uteri in this study. It was found that there was a significant increase in the size of nuclei in leiomyomas compared to the normal myometrial nuclei from the same patient. The myometrial nuclei from pregnant uteri were also significantly larger than those from non-gravid uteri.

No description available.

This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).

No description available.

by Lai-pang Law. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 148-182). / ABSTRACT / LIST OF ILLUSTRATIONS / LIST OF TABLES / ACKNOWLEDGEMENTS / ABBREVIATIONS / Chapter CHAPTER I --- INTRODUCTION --- p.1 / Chapter CHAPTER II --- LITERATURE REVIEW --- p.4 / Chapter 2.1 --- The uterus and its changes in the normal menstrual cycle / Chapter 2.2 --- Anatomy and physiology of normal myometrium / Chapter 2.3 --- Clinical features and management of uterine leiomyoma / Chapter 2.4 --- Pathology of human uterine leiomyoma / Chapter 2.5 --- The relationship between growth fractions and ER in breast carcinoma / Chapter 2.6 --- Steroid receptors and epidermal growth factor receptor / Chapter 2.6.1 --- Steroid receptors / Chapter 2.6.2 --- Epidermal growth factor receptor / Chapter 2.7 --- "Structures of oestrogen receptor, progesterone receptor, Ki-67 and epidermal growth factor receptor" / Chapter 2.7.1 --- The structure of oestrogen receptor / Chapter 2.7.2 --- The structure of progesterone receptor / Chapter 2.7.3 --- The structure of Ki-67 / Chapter 2.7.4 --- The structure of epidermal growth factor receptor / Chapter 2.8 --- "Antibodies to steroid receptors, monoclonal Ki-67 and epidermal growth factor receptor" / Chapter 2.8.1 --- Steroid receptors / Chapter 2.8.2 --- Monoclonal Ki-67 / Chapter 2.8.3 --- Epidermal growth factor receptor / Chapter 2.9 --- "Functions of steroid receptors, Ki-67 and epidermal growth factor receptor" / Chapter 2.9.1 --- The functions of steroid receptors / Chapter 2.9.2 --- The functions of Ki-67 / Chapter 2.9.3 --- The functions of epidermal growth factor receptor / Chapter 2.10 --- Cell cycle / Chapter 2.11 --- Immunohistochemistry / Chapter 2.11.1 --- Introduction / Chapter 2.11.2 --- Methodology of immunostaining / Chapter 2.11.3 --- Avidin-biotin-peroxidase complex technique / Chapter 2.11.4 --- Chromogens / Chapter 2.11.5 --- Enhancement methods / Chapter 2.11.6 --- Fixation for immunohistochemistry / Chapter CHAPTER III --- MATERIALS AND METHODS --- p.63 / Chapter 3.1 --- Reagents and chemicals / Chapter 3.1.1 --- Primary monoclonal antibodies / Chapter 3.1.2 --- Secondary antibodies / Chapter 3.1.3 --- Avidin-biotin complex / Chapter 3.1.4 --- DAB solution / Chapter 3.1.5 --- Buffers / Chapter 3.1.6 --- Miscellaneous / Chapter 3.2 --- Patients and specimens / Chapter 3.2.1 --- Specimen collection / Chapter 3.2.2 --- Preparation of specimens / Chapter 3.3 --- Immunohistochemical staining / Chapter 3.3.1 --- Slide preparation / Chapter 3.3.2 --- Antigen retrieval / Chapter 3.3.3 --- Procedures of immunohistochemical staining / Chapter 3.3.4 --- Interpretation of immunostaining / Chapter CHAPTER IV --- RESULTS --- p.80 / Chapter 4.1 --- Clinical information / Chapter 4.2 --- Oestrogen receptor / Chapter 4.3 --- Progesterone receptor / Chapter 4.4 --- Epidermal growth factor receptor / Chapter 4.5 --- Ki-67 / Chapter CHAPTER V --- DISCUSSION --- p.120 / Chapter 5.1 --- Methods and interpretation of the results / Chapter 5.1.1 --- The advantages of the immunohistochemical staining technique / Chapter 5.1.2 --- Interpretation and reporting of immunohistochemical results / Chapter 5.1.3 --- Interpretation of the results by semi- quantitative assessment and statistical analysis / Chapter 5.2 --- The status of steroid receptors in uterine leiomyoma / Chapter 5.2.1 --- ER status in uterine leiomyoma and normal myometrium / Chapter 5.2.2 --- PR status in uterine leiomyoma and normal myometrium / Chapter 5.3 --- EGF-R status in uterine leiomyoma / Chapter 5.4 --- Ki-67 status in uterine leiomyoma and normal myometrium / Chapter 5.5 --- "The relationship between steroid receptors, Ki-67, EGF-R and uterine leiomyoma growth" / Chapter 5.6 --- Biological indices in the assessment of tumor / Chapter 5.7 --- Microwave technology in immunohistology for surgical pathology / Chapter CHAPTER VI --- CONCLUSIONS --- p.144 / REFERENCES --- p.148