Japan obtained equal status with the west imperialism, like Britain and North America, after defeating Russia in 1905. For promoting their mainland policy, they set up the semi-official and semi-private company- South Manchuria Railway (SMR). It owned a huge Research Bureau to investigate some aspects like railway, natural resources, transportation and military, not only for satisfying colonial goal but also for producing rich, valuable and multidimensional knowledge .During forty-years, the Research Bureau attracted and enrolled many Japanese who had interests on, or preference to China. Japanese took this process as a approach or domain to contact with China. This process also reflected their own ideology, image and relative location to China by the rise of the Empire of Japan. This dissertation starts from reviewing important research results, such as Shina Resistance Report, Nōson Chūgoku Kankō Chōsa Surveys. The discussion goes further to four researchers in the SMR: Shūmei Ōkawa, Tachibana Shiraki, Amano Waranosuke and Itō Takeo, who were with various academic background and even standpoint. The investigators attempted to construct their own research approach and meanwhile suffered from dealing it with the subjective power structure. The last section focuses on the academic debate after the war time, in term of controversial interpretation about Mantetsu knowledge from Japanese Sinology. From these three perspectives, the dissertation intends to take Mantetsu as a example to portray the context, power relationship and conflicts within research institute in Japan. It would therefore generalize objectivity, autonomy and path-dependence from the Mantetsu knowledge.