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Evaluation of diazepam in conjunction with nitrous oxide-oxygen for relief of anxiety in young pedodontics patients a thesis submitted in partial fulfillment ... pedodontics /Smith, Bradley R. January 1981 (has links)
Thesis (M.S.)--University of Michigan, 1981.
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Targets for pharmacological intervention in the bladder and urethraWaldeck, Kristian. January 1998 (has links)
Thesis (doctoral)--Lund University, 1998. / Added t.p. with thesis statement inserted. Includes bibliographical references.
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Occupation and reproductive health of female dentists the relationships of nitrous oxide and amalgam (mercury) with spontaneous abortion /Kaste, Linda Marie. January 1996 (has links)
Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 1996. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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Evaluation of diazepam in conjunction with nitrous oxide-oxygen for relief of anxiety in young pedodontics patients a thesis submitted in partial fulfillment ... pedodontics /Smith, Bradley R. January 1981 (has links)
Thesis (M.S.)--University of Michigan, 1981.
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Occupation and reproductive health of female dentists the relationships of nitrous oxide and amalgam (mercury) with spontaneous abortion /Kaste, Linda Marie. January 1996 (has links)
Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 1996. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
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The effects of nitrous oxide during pediatric dental sedation with oral transmucosal fentanyl citrate and hydroxyzine pamoatePilipowicz, Orest. January 2006 (has links)
Thesis (M.S.)--University of Michigan, 2006. / Includes bibliographical references (leaves 116-122).
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Targets for pharmacological intervention in the bladder and urethraWaldeck, Kristian. January 1998 (has links)
Thesis (doctoral)--Lund University, 1998. / Added t.p. with thesis statement inserted. Includes bibliographical references.
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Phytoremediation of Nitrous Oxide: Expression of Nitrous Oxide Reductase from Pseudomonas Stutzeri in Transgenic Plants and Activity thereofWan, Shen January 2012 (has links)
As the third most important greenhouse gas, nitrous oxide (N2O) is a stable greenhouse gas and also plays a significant role in stratospheric ozone destruction. The primary anthropogenic source of N2O stems from the use of nitrogen in agriculture, with soils being the major contributors. Currently, the annual N2O emissions from this “soil–microbe-plant” system is more than 2.6 Tg (one Tg equals a million metric tons) of N2O-N globally. My doctoral studies aimed to explore innovative strategies for N2O mitigation, in the context of environmental microbiology’s potential contribution to alleviating global warming. The bacterial enzyme nitrous oxide reductase (N2OR), naturally found in some soils, is the only known enzyme capable of catalyzing the final step of the denitrification pathway, conversion of N2O to N2. Therefore, to “scrub” or reduce N2O emissions, bacterial N2OR was heterologously expressed inside the leaves and roots of transgenic plants. Others had previously shown that the functional assembly of the catalytic centres (CuZ) of N2OR is lacking when only nosZ is expressed in other bacterial hosts. There, coexpression of nosZ with nosD, nosF and nosY was found to be necessary for production of the catalytically active holoenzyme. I have generated transgenic tobacco plants expressing the nosZ gene, as well as tobacco plants in which the other four nos genes were coexpressed. More than 100 transgenic tobacco lines, expressing nosZ and nosFLZDY under the control of rolD promoter and d35S promoter, have been analyzed by PCR, RT-PCR and Western blot. The activity of N2OR expressed in transgenic plants, analyzed with the methyl viologen-linked enzyme assay, showed detectable N2O reducing activity. The N2O-reducing patterns observed were similar to that of the positive control purified bacterial N2OR. The data indicated that expressing bacterial N2OR heterologously in plants, without the expression of the accessory Nos proteins, could convert N2O into inert N2. This suggests that atmospheric phytoremediation of N2O by plants harbouring N2OR could be invaluable in efforts to reduce emissions from crop production fields.
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Coupled Abiotic and Biotic Cycling of Nitrous OxideJanuary 2020 (has links)
abstract: Nitrous oxide (N2O) is an important greenhouse gas and an oxidant respired by a
diverse range of anaerobic microbes, but its sources and sinks are poorly understood. The overarching goal of my dissertation is to explore abiotic N2O formation and microbial N2O consumption across reducing environments of the early and modern Earth. By combining experiments as well as diffusion and atmospheric modeling, I present evidence that N2O production can be catalyzed on iron mineral surfaces that may have been present in shallow waters of the Archean ocean. Using photochemical models, I showed that tropospheric N2O concentrations close to modern ones (ppb range) were possible before O2 accumulated. In peatlands of the Amazon basin (modern Earth), unexpected abiotic activity became apparent under anoxic conditions. However, care has to be taken to adequately disentangle abiotic from biotic reactions. I identified significant sterilant-induced changes in Fe2+ and dissolved organic matter pools (determined by fluorescence spectroscopy). Among all chemical and physical sterilants tested, γ - irradiation showed the least effect on reactant pools. Targeting geochemically diverse peatlands across Central and South America, I present evidence that coupled abiotic and biotic cycling of N2O could be a widespread phenomenon. Using isotopic tracers in the field, I showed that abiotic N2O fluxes rival biotic ones under in-situ conditions. Moreover, once N2O is produced, it is rapidly consumed by N2O-reducing microbes. Using amplicon sequencing and metagenomics, I demonstrated that this surprising N2O sink potential is associated with diverse bacteria, including the recently discovered clade II that is present in high proportions at Amazonian sites based on nosZ quantities. Finally, to evaluate the impact of nitrogen oxides on methane production in peatlands, I characterized soil nitrite (NO2–) and N2O abundances along soil profiles. I complemented field analyses with molecular work by deploying amplicon-based 16S rRNA and mcrA sequencing. The diversity and activity of soil methanogens was affected by the presence of NO2– and N2O, suggesting that methane emissions could be influenced by N2O cycling dynamics. Overall, my work proposes a key role for N2O in Earth systems across time and a central position in tropical microbial ecosystems. / Dissertation/Thesis / Doctoral Dissertation Microbiology 2020
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Greenhouse gas emissions from Scottish arable agriculture and the potential for biochar to be used as an agricultural greenhouse gas mitigation optionWinning, Nicola Jane January 2015 (has links)
Nitrous oxide (N2O) is a powerful greenhouse gas (GHG) which has a global warming potential 296 times greater than that of carbon dioxide (CO2). Agriculture is a major source of N2O and in the UK approximately 71 % of N2O emissions are produced by agricultural soils, mainly as a result of the application of nitrogenous fertilisers. Despite previous research into agricultural N2O emissions which has demonstrated that N2O emissions have high spatial and temporal variability, there is still a lack of knowledge surrounding the factors that influence the magnitude of emissions from agricultural soils. Agricultural N2O emissions for the UK’s annual GHG inventory are currently estimated using a 1.25 % emission factor (EF) (to be decreased to 1 % in 2015) which assumes that 1.25 % of applied nitrogen (N) fertiliser is emitted as N2O. The EF does not take into account influencing factors such as location or fertiliser type. Mitigation of N2O emissions is vital if future climate change is to be prevented, yet this must also be combined with the need to intensify agricultural production to feed the increasing global population. Biochar which is a carbon rich material produced during the pyrolysis of biomass has been identified as a potentially useful soil amendment with the ability to mitigate N2O emissions. However, most previous research has focused on laboratory scale experiments and there is a need to investigate the use of biochar in a field environment. Other N2O mitigation options such as nitrification inhibitors, or altering fertiliser management practices, require testing under different conditions to assess their suitability for use. This thesis aims to investigate a). The factors affecting N2O emissions from synthetically and organically fertilised arable soils, and b). To explore the potential of various N2O mitigation options for arable systems, including biochar. This thesis firstly investigates N2O emissions from synthetically fertilised arable soil. Varying application rates of ammonium nitrate fertiliser were applied to a Scottish arable soil during a year long field experiment and the effects of mitigation options such as a nitrification inhibitor (DCD) were assessed. N2O emissions were shown to be significantly affected by soil water filled pore space and the 1.25 % EF was demonstrated to be generally greater than those calculated in this experiment. The use of DCD significantly decreased N2O emissions and crop yields. A second year long field experiment was carried out to investigate N2O and NH3 emissions from an organically fertilised arable soil and to explore the effect of the timing, form and method of organic fertiliser application on emissions and EFs. Slurry, poultry litter, layer manure and farmyard manure were applied in the autumn and the spring. Cumulative N2O emissions were generally greater from the autumn applications and NH3 emissions were greater from the spring applications, due to wetter soil conditions and incorporation of fertiliser during the autumn. The type of fertiliser applied affected the magnitude of emissions with the greatest cumulative N2O and NH3 emissions from the layer manure. The method of fertiliser application had no effect on emissions. The following experiment investigated the ability of different biochars to retain N from a solution and the effect of biochar particle size on retention. A batch sorption experiment was used to test the affinity and capacity of six biochars for ammonium (NH4+) and nitrate (NO3-) from different concentrations of NH4NO3 solution. All of the biochars studied demonstrated the ability to retain NH4+ and NO3- from solution although greater NH4+ retention was observed. Differences in biochar affinity for N could be explained by pyrolysis temperature, but there was no effect of particle size or pH. Oil seed rape straw biochar was demonstrated to have the greatest NH4+ and NO3- retention capacity and as such was chosen for use in the next experiment. This work investigated the potential for oil seed rape straw biochar to decrease emissions of N2O, CH4 and CO2 from stored slurry and whether any GHG mitigation effects would continue following application of the slurry to arable soil. The effect on emissions of amending the biochar and slurry mixture with DCD after application to the soil was also explored. There was no significant effect of the biochar on GHG emissions from the stored slurry although the slurry initially acted as a sink for N2O and CO2. There were no significant differences between emissions from any treatments following application to the soil. The overall results of these studies indicate that N2O emissions are highly dependent on weather conditions, and hence location, in addition to fertiliser type and application timing. It was concluded that the use of a standard 1.25 % EF for synthetic and organic N fertiliser applications for the whole of the UK is inappropriate. Mitigation options such as the use of DCD, altering fertiliser application season or fertiliser type have been shown to possess the potential to mitigate N2O emissions but tradeoffs between N2O and NH3 emissions, and impacts on crop yields must be considered. Biochar was demonstrated to retain NH4+ and NO3- ions and this property may account for biochar’s N2O mitigation capabilities as observed by previous researchers. However, if N retention is taking place, the N appears to still be available for production of N2O and crop uptake.
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