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Sensitivity control of optical fiber biosensors utilizing turnaround point long period gratings with self-assembled polymer coatingsGifford, Erika Lea 25 July 2008 (has links)
Biosensors have a multitude of important applications in basic research, environmental monitoring, biodefense, and medicine. This research aims to show that Ionic Self-Assembled Multilayers (ISAMs) adsorbed on Long Period Gratings (LPGs) can serve as a highly sensitive, robust, inexpensive optical-based biosensor platform. The ISAM technique is a layer-by-layer deposition method that builds nanometer-thick films based on the principle of Coulomb attraction between oppositely charged polyelectrolyte solutions while LPGs cause strong attenuation bands that enable an optical fiber to be extremely sensitive to changes in the surrounding environment. LPGs have been shown to be highly sensitive to the adsorption of nanoscale self-assembled films on the optical fiber cladding surface. In this work, we utilize Turnaround Point (TAP) LPGs, which possess even greater sensitivity than standard LPGs. This thesis focuses on evaluation of approaches to increasing the sensitivity of the sensor platfom, implementation of a biosensor for detection of several biomolecules, and preliminary evaluation of the potential for pH sensing.
For a thin-film coated TAP LPG, we have demonstrated that shifts in the transmitted light intensity at the resonant wavelength of the LPG can result from the variation in film thickness and/or refractive index. We have observed decreases in intensity as large a 7 dB for one bilayer of ISAM film (~1 nm), which corresponds to an 80% decrease in the transmitted light intensity at the resonant wavelength. We have also shown that the sensitivity of the TAP LPG sensor can be increased by implementing nm-thick ISAM films that have a refractive index greater than silica. Furthermore, it is shown that incorporation of silica nanoparticles into the ISAM films significantly increases sensitivity through increased surface area and thickness.
The biotin-streptavidin system was used as a model for implementaion and optimization of the ISAM-coated TAP LPG biosensor platform. Through evaluation of various biotin derivatives to maximize the amount functionalized onto the ISAM film, optimization of the ISAM film properties, and use of LPGs designed for higher sensitivity, the minimum detectable concentration of streptavidin was decreased from 0.0125 mg/ml to 12.0 ng/ml. The biosensor platform was then tested on prostate specific antigen (PSA), which is used as a clinical marker for early diagnosis of potential prostate cancer. Using a direct crosslinking approach of the monoclonal antibody to PSA into the ISAM film, a sensitivity level of 11.64 ng/ml PSA was obtained through combined optimization of the ISAM film and antibody surface coverage. Finally, the potential of ISAM TAP LPGs as pH sensors was examined based on the pH dependent swelling of ISAM films. / Ph. D.
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Ionic Self-Assembled Multilayers Adsorbed on Long Period Fiber Gratings for Use as BiosensorsWang, Zhiyong 27 December 2005 (has links)
Biosensors have widespread applications in many areas. Currently the Surface Plasmon Resonance (SPR) biosensor is one of the most prevalent types of biosensor. However, it has several disadvantages such as being delicate, expensive, and non-portable. Ionic Self-Assembled Multilayers (ISAMs) adsorbed on Long Period Fiber Gratings (LPGs) provides an attractive platform for building optical sensors, which could potentially overcome the disadvantages of SPR biosensors. The ISAM technique is a type of layer-by-layer deposition technique for building nanoscale thin films. An LPG is a type of fiber device that is sensitive to physical property changes of the ambient environment. LPGs have been extensively investigated for use as optical sensors. We have carried out a study on combining these two techniques to build efficient biosensors.
In this thesis, we demonstrate ultra-sensitive LPGs whose attenuation can be changed by 25 dB (~99.7%) over a 48-nm spectral band, with ambient-index changes of only 2.7E-4. The device schematic allows arbitrarily high index sensitivities to be achieved, which makes it an attractive platform for realizing sensors and modulators that respond to small index changes. For a thin-film coated LPG, we find theoretically that the resonant wavelength shift of the LPG can result from either the variation of the thickness of the film and/or the variation of its refractive index. Furthermore, results illustrate that the sensitivity of the sensor could be enhanced using a nm-thick thin-film (e.g. ISAM films) whose refractive index is greater than silica. Experimentally, we demonstrate the fabrication of nm-thick ISAM films deposited on LPGs, which induces dramatic shifts in the resonant wavelength. The refractive index and the thickness of the ISAM film was precisely controlled by altering the relative fraction of the anionic and cationic materials combined with layer-by-layer deposition. Finally, we demonstrate that ISAM-coated LPGs can function effectively as biosensors by using the biotin-streptavidin system. These demonstrations confirm that the ISAM-LPG scheme provides a thermally-stable, reusable, and robust platform for building efficient optical sensors. / Ph. D.
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