The presented work investigates the mechanisms of propagation of heat and moisture in the structure of heat-insulating materials based on natural fibers. The main aim of this work is the experimental verification of theoretical patterns, which are involved in the transport of heat and moisture in fibrous materials, especially the influence of fibers thickness and density of the fibrous materials. To widen outputs of the work were selected different kinds of natural fiber materials of vegetable and animal origin. The results of the work should help to optimize the production processes of natural insulation materials considering their optimal thermal properties. From the data collected are deduced general conclusions to better understanding the behavior of these materials.