No description available.

Natural resource managers in the Great Smoky Mountains National Park requested the assistance of the University of Tennessee Department of Civil and Environmental Engineering to assess Abrams Creek for potential stream restoration needs. A presumed, unstable study reach and a stable reference reach were identified on Abrams Creek in Cades Cove. Chemical, biological and physical assessments were completed on Abrams Creek in order to evaluate ecological health and channel stability of the stream. Water quality and ecological (fish and habitat surveys) data acquired by National Park Service, Tennessee Valley Authority and the University of Tennessee were assessed. The physical assessment included two approaches; they were: 1) empirical or reference reach approach; and 2) analytical or non-reference reach approach. The current empirical technique used was the analog Natural Channel Design. The current analytical techniques were the hydraulic, sediment transport and erosion models (HEC-RAS, CONCEPTS). These physical assessment techniques were used to determine bankfull or effective flows, sedimentation, stream stability, and ecohydraulics. In addition to using these techniques for the Park’s management objectives, they were applied to both reaches for comparison in order to clarify areas where professional judgment may introduce uncertainty. From comprehensive physical assessments no system wide instabilities were observed but some riparian area differences and localized erosion were noted. Recommendations for potential restoration needs on Abrams Creek include localized stabilization of stream banks and vegetating the riparian corridor along the study reach.

Natural resource managers in the Great Smoky Mountains National Park requested the assistance of the University of Tennessee Department of Civil and Environmental Engineering to assess Abrams Creek for potential stream restoration needs. A presumed, unstable study reach and a stable reference reach were identified on Abrams Creek in Cades Cove. Chemical, biological and physical assessments were completed on Abrams Creek in order to evaluate ecological health and channel stability of the stream. Water quality and ecological (fish and habitat surveys) data acquired by National Park Service, Tennessee Valley Authority and the University of Tennessee were assessed. The physical assessment included two approaches; they were: 1) empirical or reference reach approach; and 2) analytical or non-reference reach approach. The current empirical technique used was the analog Natural Channel Design. The current analytical techniques were the hydraulic, sediment transport and erosion models (HEC-RAS, CONCEPTS). These physical assessment techniques were used to determine bankfull or effective flows, sedimentation, stream stability, and ecohydraulics. In addition to using these techniques for the Park’s management objectives, they were applied to both reaches for comparison in order to clarify areas where professional judgment may introduce uncertainty. From comprehensive physical assessments no system wide instabilities were observed but some riparian area differences and localized erosion were noted. Recommendations for potential restoration needs on Abrams Creek include localized stabilization of stream banks and vegetating the riparian corridor along the study reach.

No description available.

No description available.

No description available.

Detailed physical profile of eight transplanted loggerhead sea turtle, Caretta caretta, nests were obtained from Virginia and northeastern North Carolina and reburied in Back Bay National Wildlife Refuge (BBNWR), Virginia Beach, Virginia during the summers of 1987, '88, and '89 to determine if the physical location of the egg within the nest had an affect on sex determination. Transplanted nests were reburied in sandy substrate at a depth of 15-60 em on south facing dunes, and a Campbell Scientific data-logger logged synchronously environmental data. The following data were collected: temperatures at various locations within a nest, net absorbed radiation, ambient temperature, rainfall (em), substrate moisture content, and tide cycles. To understand the determination of sex, a time series analyses was employed to explain the variability, the periodicity, and the irregular oscillations of the temperature data. The regression analysis, using the periodicity of the spring/neap cycles, indicated a significant diurnal and spring/neap tidal affect. The 29.5 and 14.7 day cycle were significant. In addition, a delayed heating affect on the dune temperatures was noted 3-5 days after the lowest tides of the full and new moon cycles which consistently occurred at approximately 3:00P.M. Temperature records in nests at BBNWR were consistent with those producing a predominance of male hatchlings (<28.0C). Temperatures which produced females did exist, but were infrequent. Maximum beach face heating resulted in elevating · temperatures from 1-3C at the 37cm depths. This affect could produce females in late summer and early fall. Physical parameters varied with depth, time of day, and season (summer/fall). The results of this study are important to conservation of sea turtles because they contribute much to the knowledge of how nest manipulation may control sex ratios. Using the approach developed in this study, a mathematical model to predict sex ratios could be developed that would be applicable for various physical regimes found on most major nesting beaches of various sea turtles around the world.

Biodiversity is what conservation biology was developed to conserve. It is the physical manifestation of life as a concept and, be it for practical or idealistic reasons, all conservationists seek to protect or, in some cases, enhance it. Because of its monolithic importance to the field, much effort has been expended trying to better measure and understand it. Recently, greater attention has been paid to the partition of diversity; the observation that the total diversity of a system (γ) can be broken down into within-site diversity (α) and between-site diversity (β). In particular, it has been noticed that the β component of diversity is not as well studied or understood as the α component. In this study I attempt to address this shortfall, by examining two questions: (1) how is β is best measured and (2) what drives β? To answer the first question, I look to find the measure of β that is most robust to sampling error. While many β indices have been proposed, few have considered how our methods of data gathering might affect those indices. Datasets collected from the real world will all likely have some sort of error within them as a result of the way they were sampled. Those errors will affect some indices more than others, and the indices that are least affected will be the most reliable for actual data. Once robust indices were identified, I used them to identify possible predictors of β in two large, national datasets. The first dataset was the National Lakes Assessment created by the USEPA, in which diatoms were sampled from over 1000 lakes across the country. The second was the eBird dataset from the Cornell Lab of Ornithology, which used citizen science to generate a continuous dataset spanning both the last decade and the boundaries of the conterminous United States. β calculated from these sources was regressed against relevant environmental variables to create a clearer understanding of the effects of the environment on the β of two very different ecological systems.

Recent work indicates that the Y-chromosome of the fruit fly Drosophila melanogaster can influence gene regulation on the autosomes and X chromosome. This newly discovered function of the Y has the potential to dramatically shape the regulatory evolution of numerous genes that reside throughout the genome; even for genes that code for both male and female traits. Given that the mechanism underlying the Y-linked influence on gene expression in D. melanogaster appears to exist in other independently evolved heterogametic sex chromosomes, the evolutionary implications of Y-linked regulatory variation (YRV) deserves to be explored. These implications include the potential for Y-chromosomes to facilitate the adaptive evolution of sexually dimorphic gene expression, and the potential for the Y to constrain evolutionary rates in both males and females (depending on the nature of the YRV effect). Unfortunately, the evolutionary implications of this potentially widespread and significant phenomenon have yet to be explored. My dissertation addresses this knowledge gap by determining the influence YRV has on the evolution of autosomally coded traits in D. melanogaster. First, we address the potential for selection to shape YRV by determining if YRV (i) exists within natural populations (i.e. where natural selection operates), and (ii) has any influence on male fitness-related autosomal traits. Second, we address if YRV can facilitate the adaptive evolution of sexually dimorphic gene expression by testing for the presence of Y-linked additive genetic variation. To this end, we investigate the physiological properties of select Y-chromosomes across multiple genetic backgrounds. Third, we address if YRV can constrain adaptive evolution for autosomally coded traits by employing artificial selection on replicate populations that contain either multiple Y-chromosomes (i.e. contain YRV) or only a single Y-chromosome (no YRV). The following studies present evidence that YRV does exist within populations where natural selection operates. We show significant levels of YRV on X-linked and autosomal immune gene expression in wild caught D. melanogaster from a single natural population. Furthermore, YRV effects on immune related genes show a significantly positive correlation to a male fly's ability to fight an immune challenge (an important aspect of organismal fitness). Estimated physiological properties of YRV support previous interpopulation studies showing strong non-additive effect dependent on the autosomal genetic background with which Y-chromosome's are paired with. Physiological epistasis can manifest as additive genetic variation on a population level, but our experimental evolution study suggest that YRV constrains rather than facilitates the evolution of the autosomal coded geotaxis behavior. Ultimately, this dissertation provides evidence that YRV has the potential to influence how autosomal traits evolve and that population level studies of YRV indicate a potential constraint to the adaptive evolution of autosomal traits. If these trends are common and YRV is a wide spread phenomenon, Y-chromosomes have the potential to influence how autosomal traits evolve.

The Florida manatee (Trichechus manatus latirostris) was recently downlisted federally from "endangered" to "threatened" despite acknowledgments of remaining threats to long term population persistence. Challenges to future manatee conservation include, but are not limited to, increases in frequency of harmful algal blooms, intensifying anthropogenic disturbance, and loss of warm-water habitat. The goals of this dissertation were 1) to assess threats to the manatee via a comprehensive, long-term (1973-2016), retrospective analysis of the manatee rescue and rehabilitation partnership (MRRP) and 2) to use seascape genetics analysis to examine whether abiotic, biotic, or anthropogenic seascape variables could significantly describe genetic distance patterns in space for this genetically depauperate population. Results from the MRRP analysis revealed that anthropogenic threats were the most significant reason for manatees to be rescued and rehabilitated. Manatees rescued due to watercraft injuries spent long periods in recovery before succumbing or being released resulting in significant expense to the rehabilitation system. Additionally, the seascape genetics analysis indicated that watercraft activity best explained spatial genetic patterns in the manatee population. It is established that anthropogenic use of watercraft negative affects manatees through the mechanisms of sub-lethal injury and mortality, and these results suggest there may be further negative impacts via the disruption of population genetic connectivity. Future management practices should seriously consider manatee/vessel interactions as watercraft strikes are costly for management, place pressure on the manatee population, and could disrupt population gene flow with potentially dire consequences. Mitigating anthropogenic impacts on the Florida manatee population is critical for future conservation and should be a primary focus.