Yes / To reproduce the natural flow topography in a laboratory environment, it is crucial to recapture its bed condition in order to ensure the accurate representation. Water-worked bed represents a state-of-the-art experimentally formed bed to imitate the natural-formed channel in most rivers or natural streams. Recently, this technique has been intensively studied through experimental and computational approaches; however, its actual influence towards the near-bed flow as compared to experimentally prepared rough bed in well-packed bedform order are still yet to be investigated deeply. This experimental study systematically investigated and compared the differences in velocity distribution and three-dimensional (3D) turbulence characteristics, including turbulence intensities and Reynolds stresses, between uniform smooth bed, laboratory-prepared rough bed and water-worked bed open channel flows. The flow comparisons were concentrated at near-bed region where clear flow behaviour change can be observed. Through these comparisons, the study inspected the characteristics of water-worked bedform thoroughly, in order to inform future experimental research that tries to reproduce natural stream behaviours. / the Major State Basic Research Development Grant No. 2013CB036402 from Tsinghua University. The support from the Major State Basic Research Development Program (973 program) of China is also greatly appreciated. We also acknowledge the National Key Research and Development Project from the Ministry of Science and Technology during the Thirteenth Five-year Plan Period (Grant No. 2017YFC0403600) and the Science and Technology Projects State Grid Corporation of China (Grant No. 52283014000T).