Yes / When using point measurement for environmental or sediment laden flows, there is well-recognised risk for not having aligned measurements that causes misinterpretation of the measured velocity data. In reality, these kinds of mismeasurement mainly happen due to the misinterpretation of bed orientation caused by the complexity of its determination in natural flows, especially in bedload laden or rough bed flows. This study proposes a novel bed realignment method to improve the measured data benchmarking by three-dimensional (3D) bed profile orientation and implemented it into different sets of experimental data. More specifically, the effects of realignment on velocity profile and streamwise turbulence structure measurements were investigated. The proposed technique was tested against experimental data collected over a water-worked and an experimentally arranged well-packed beds. Different from the well-packed rough bed, the water-worked bed has been generated after long sediment transport and settling and hence can be used to verify the proposed bed-alignment technique thoroughly. During the flow analysis, the corrected velocity, turbulence intensity and Reynolds stress profiles were compared to the theoretical logarithmic law, exponential law and linear gravity (universal Reynolds stress distribution) profiles, respectively. It has been observed that the proposed method has improved the agreement of the measured velocity and turbulence structure data with their actual theoretical profiles, particularly in the near-bed region (where the ratio of the flow measurement vertical distance to the total water depth, z/h, is limited to ≤0.4).