It is a research of the move trajectory of the ciliates while feeding the food, in order to estimate, differentiate from the movement behavior under different environments. First, discuss the differently distinguish with the single indicator. Second, discuss with integrate four kinds of indicator whether can distinguish differently. Finally, combine the indicator data and through different analysis technology look out the features of movement behavior, expect to be able to look out suitable information and knowledge from the indicator data. After deal with analytical technology, the result of decision tree is most suitable for predicted and have credibilities. If according to energy of biological, the analysis result is similar to optimal foraging theory. And learn from result under different condition, the movement behavior of the ciliates similar to the optimal foraging theory. In the matter of the result of analysis technology, data of the density of low food similar to data of the density of extremely high food. Besides, data of medium food and high food are analogous. The rule of decision tree can distinguish the density of different food, and can offer follow-up study to distinguish the environmental conditions. Those models are evaluated by predicting accuracies, and rules extracted from decision tree models are also of great help to prediction as well.