DNA methylation-dependent gene silencing, catalyzed by DNA methyltransferases (DNMTs) and mediated by methyl binding domain proteins (MBDs) and histone deacetylases (HDACs), is essential for mammalian development, with the nervous system demonstrating particular sensitivity to perturbations. Little is known, however, about the role of DNA methylation in the stage-specific differentiation of neurons. In the olfactory epithelium (OE), where neurogenesis is continuous and the cells demonstrate a laminar organization with a developmental hierarchy, we identified sequential, transitional stages of differentiation likely mediated by different DNMT, MBD and HDAC family members. Biochemically, HDAC1 and HDAC2 associate with repressor complexes recruited by both MBD2 and MeCP2. HDAC1 and HDAC2, however, are divergently expressed in the OE, a pattern that is recapitulated in the brain. Rather than simultaneous inclusion in a complex, therefore, the individual association of HDAC1 or HDAC2 may provide specificity to a repressor complex in different cell types. Furthermore, distinct transitional stages of differentiation are perturbed in the absence of MBD2 or MeCP2. MeCP2 is expressed in the most apical immature olfactory receptor neurons (ORNs), and is up-regulated with neuronal maturation. In the MeCP2 null OE there is a transient delay in ORN maturation and an increase in neurons of an intermediate developmental stage. Two protein variants of MBD2 are expressed in the OE, with MBD2b expressed in cycling progenitor cells and MBD2a in the maturing ORNs. MBD2 null ORNs undergo increased apoptotic cell death. There is also a significant increase in proliferating progenitors in the MBD2 null OE, likely due, at least in part, to feedback from the dying ORNs, acting to up-regulate neurogenesis. Increased cell cycling in the MBD2 null is also observed post-lesion, however, in the absence of feedback back from the ORNs, a phenotype that is recapitulated by an acute inhibition of HDACs with valproic acid. Therefore, disruptions at both transitional stages of ORN differentiation are likely in the MBD2 null mouse. Together, these results provide the first evidence for a sequential recruitment of different MBD proteins and repressor complexes at distinct transitional stages of neuronal differentiation.

This research introduces neural networks (NN) as a novel approach towards aiding biocomposite materials processing. At its core, the aim of the research was to investigate NN usage as a tool for advancing the field of biocomposites. Empirical data was generated for compression-molded flax fiber and High Density Polyethylene (HDPE) matrix based biocomposite materials. In an attempt to create the NN model, tensile strength, impact strength, hardness, bending strength, and density were provided to the NN as inputs. These inputs were processed through multiple layers of the NN, and contributed to the prediction of the composition (fiber loading percentage) and operating parameter (pressure in MPa) as output. In précis, NNs use was investigated to predict composition and operational parameter for biocomposites production when the desired mechanical properties of the biocomposites were available. Flax (Linum usitatissimum) fiber biocomposite boards were manufactured using chemically pretreated flax fiber and high density polyethylene (HDPE). After extensive preprocessing (combing and size reduction to 2 mm particles) and pretreatment regimen - flax fiber was mixed with HDPE and extruded using a laboratory scale single screw extruder. Extrudates generated from the extruder were again ground to 2 mm particles. Ground extrudates from different sample sets were exposed to a compression molding unit. The mold was put under two sets of pressures, (variable operating parameters) for all individual fiber loading. These boards were used to determine the mechanical properties tensile force, impact force, hardness, bending, and density. For verification and analysis of the mechanical properties, Microsoft Office Excel and a statistical software package SAS were used. After verification five different multilayer neural networks, i.e., cascade forward neural network, feedforward backpropagation neural network, neural unit (single layer, single neuron), feedforward time delay neural network and NARX, were trained and evaluated for performance. Ultimately, the feedforward backpropagation NN (FFBPNN) was selected as the most efficient. After rigorous testing, the FFBPNN trained by the TRAINSCG algorithm (Matlab ®) was selected to generate prediction results that were the most suitable, fast and accurate. Once the selection and training of the NN architecture was complete, biocomposite materials prediction was performed. From 9 separate input sets, NNs provided overall prediction error between 2 - and 4%. This was the same amount of error that was observed in the training of the neural network. It was concluded that the neural network approach for the experimental design and operational conditions were satisfied.

Knowledge of the mass of the manipulated load (i.e. payload) in off-highway machines is useful information for a variety of reasons ranging from knowledge of machine stability to ensuring compliance with transportion regulations. This knowledge is difficult to ascertain however. This dissertation concerns itself with delineating the motivations for, and difficulties in development of a dynamic payload weighing algorithm. The dissertation will describe how the new type of dynamic payload weighing algorithm was developed and progressively overcame some of these difficulties.<p> The payload mass estimate is dependent upon many different variables within the off-highway vehicle. These variables include static variability such as machining tolerances of the revolute joints in the linkage, mass of the linkage members, etc as well as dynamic variability such as whole-machine accelerations, hydraulic cylinder friction, pin joint friction, etc. Some initial effort was undertaken to understand the static variables in this problem first by studying the effects of machining tolerances on the working linkage kinematics in a four-wheel-drive loader. This effort showed that if the linkage members were machined within the tolerances prescribed by the design of the linkage components, the tolerance stack-up of the machining variability had very little impact on overall linkage kinematics.<p> Once some of the static dependent variables were understood in greater detail significant effort was undertaken to understand and compensate for the dynamic dependent variables of the estimation problem. The first algorithm took a simple approach of using the kinematic linkage model coupled with hydraulic cylinder pressure information to calculate a payload estimate directly. This algorithm did not account for many of the aforementioned dynamic variables (joint friction, machine acceleration, etc) but was computationally expedient. This work however produced payload estimates with error far greater than the 1% full scale value being targeted. Since this initial simplistic effort met with failure, a second algorithm was needed. The second algorithm was developed upon the information known about the limitations of the first algorithm. A suitable method of compensating for the non-linear dependent dynamic variables was needed. To address this dilemma, an artificial neural network approach was taken for the second algorithm. The second algorithms construction was to utilise an artificial neural network to capture the kinematic linkage characteristics and all other dynamic dependent variable behaviour and estimate the payload information based upon the linkage position and hydraulic cylinder pressures. This algorithm was trained using emperically collected data and then subjected to actual use in the field. This experiment showed that that the dynamic complexity of the estimation problem was too large for a small (and computationally feasible) artificial neural network to characterize such that the error estimate was less than the 1% full scale requirement.<p> A third algorithm was required due to the failures of the first two. The third algorithm was constructed to ii take advantage of the kinematic model developed and utilise the artificial neural networks ability to perform nonlinear mapping. As such, the third algorithm developed uses the kinematic model output as an input to the artificial neural network. This change from the second algorithm keeps the network from having to characterize the linkage kinematics and only forces the network to compensate for the dependent dynamic variables excluded by the kinematic linkage model. This algorithm showed significant improvement over the previous two but still did not meet the required 1% full scale requirement. The promise shown by this algorithm however was convincing enough that further effort was spent in trying to refine it to improve the accuracy.<p> The fourth algorithm developed proceeded with improving the third algorithm. This was accomplished by adding additional inputs to the artificial neural network that allowed the network to better compensate for the variables present in the problem. This effort produced an algorithm that, when subjected to actual field use, produced results very near the 1% full scale accuracy requirement. This algorithm could be improved upon slightly with better input data filtering and possibly adding additional network inputs.<p> The final algorithm produced results very near the desired accuracy. This algorithm was also novel in that for this estimation, the artificial neural network was not used soley as the means to characterize the problem for estimation purposes. Instead, much of the responsibility for the mathematical characterization of the problem was placed upon a kinematic linkage model that then fed its own payload estimate into the neural network where the estimate was further refined during network training with calibration data and additional inputs. This method of nonlinear state estimation (i.e. utilising a neural network to compensate for nonlinear effects in conjunction with a first principles model) has not been seen previously in the literature.

Electrodes implanted into neural systems are known to degrade due to encapsulation by surrounding tissues. The mechanisms of electrode-tissue interactions and prediction of the behavior of electrode are yet to be achieved. This research will aim at establishing the fundamental knowledge of interfacial interactions between the host biological environment and an implanted electrode. We will identify the dynamic mechanisms of such interfacial interactions. Quantitative analysis of the electrical properties of interface will be conducted using Electrochemical Impedance Spectroscopy (EIS). Results will be used to develop a general model to interpret electrical circuitry of the interface. This is expected to expand our understanding in the effects of interfacial interactions to the charge transport. The interfacial interactions of an implanted electrode with neural system will be studied in two types of electrodes: silver and graphene coated. The interfacial impedance of both samples will be studied using EIS. The development of the cellular interaction will be investigated using histological procedure. X-ray photoemission spectroscopy (XPS) will be employed to study the chemical effects on the silver electrodes. Atomic force microscopy and Raman spectroscopy will be used for material characterization of graphene-coated electrodes. In the study of silver electrode, two mechanisms affecting the interfacial impedance are proposed. First is the formation of silver oxide. The other is the immuno-response of tissue encapsulation. Histological results suggest that higher cell density cause higher impedance magnitude at the interface. It is also found that the cellular encapsulation dominates the increase in impedance for longer implanted time. In the study of graphene-coated electrode, it is found that the graphene can strongly prevent the metal substrate from being oxidized. It not only provides good electrical conductivity for signal transport, but also reduces the speed of the accumulation of tissue around the electrode. Such characteristics of graphene have great potential in the application of neural implant. Finally, the dynamic mechanisms of biological interaction are proposed. A model is also developed to represent the general circuitry of the interface between an implanted electrode and the neural system. The model has three major components, which are interfacial double layer, cellular encapsulation, and the substrate. The model presented in this study can compensate for selection and prediction of materials and their behaviors.

Knowledge of the mass of the manipulated load (i.e. payload) in off-highway machines is useful information for a variety of reasons ranging from knowledge of machine stability to ensuring compliance with transportion regulations. This knowledge is difficult to ascertain however. This dissertation concerns itself with delineating the motivations for, and difficulties in development of a dynamic payload weighing algorithm. The dissertation will describe how the new type of dynamic payload weighing algorithm was developed and progressively overcame some of these difficulties.<p> The payload mass estimate is dependent upon many different variables within the off-highway vehicle. These variables include static variability such as machining tolerances of the revolute joints in the linkage, mass of the linkage members, etc as well as dynamic variability such as whole-machine accelerations, hydraulic cylinder friction, pin joint friction, etc. Some initial effort was undertaken to understand the static variables in this problem first by studying the effects of machining tolerances on the working linkage kinematics in a four-wheel-drive loader. This effort showed that if the linkage members were machined within the tolerances prescribed by the design of the linkage components, the tolerance stack-up of the machining variability had very little impact on overall linkage kinematics.<p> Once some of the static dependent variables were understood in greater detail significant effort was undertaken to understand and compensate for the dynamic dependent variables of the estimation problem. The first algorithm took a simple approach of using the kinematic linkage model coupled with hydraulic cylinder pressure information to calculate a payload estimate directly. This algorithm did not account for many of the aforementioned dynamic variables (joint friction, machine acceleration, etc) but was computationally expedient. This work however produced payload estimates with error far greater than the 1% full scale value being targeted. Since this initial simplistic effort met with failure, a second algorithm was needed. The second algorithm was developed upon the information known about the limitations of the first algorithm. A suitable method of compensating for the non-linear dependent dynamic variables was needed. To address this dilemma, an artificial neural network approach was taken for the second algorithm. The second algorithms construction was to utilise an artificial neural network to capture the kinematic linkage characteristics and all other dynamic dependent variable behaviour and estimate the payload information based upon the linkage position and hydraulic cylinder pressures. This algorithm was trained using emperically collected data and then subjected to actual use in the field. This experiment showed that that the dynamic complexity of the estimation problem was too large for a small (and computationally feasible) artificial neural network to characterize such that the error estimate was less than the 1% full scale requirement.<p> A third algorithm was required due to the failures of the first two. The third algorithm was constructed to ii take advantage of the kinematic model developed and utilise the artificial neural networks ability to perform nonlinear mapping. As such, the third algorithm developed uses the kinematic model output as an input to the artificial neural network. This change from the second algorithm keeps the network from having to characterize the linkage kinematics and only forces the network to compensate for the dependent dynamic variables excluded by the kinematic linkage model. This algorithm showed significant improvement over the previous two but still did not meet the required 1% full scale requirement. The promise shown by this algorithm however was convincing enough that further effort was spent in trying to refine it to improve the accuracy.<p> The fourth algorithm developed proceeded with improving the third algorithm. This was accomplished by adding additional inputs to the artificial neural network that allowed the network to better compensate for the variables present in the problem. This effort produced an algorithm that, when subjected to actual field use, produced results very near the 1% full scale accuracy requirement. This algorithm could be improved upon slightly with better input data filtering and possibly adding additional network inputs.<p> The final algorithm produced results very near the desired accuracy. This algorithm was also novel in that for this estimation, the artificial neural network was not used soley as the means to characterize the problem for estimation purposes. Instead, much of the responsibility for the mathematical characterization of the problem was placed upon a kinematic linkage model that then fed its own payload estimate into the neural network where the estimate was further refined during network training with calibration data and additional inputs. This method of nonlinear state estimation (i.e. utilising a neural network to compensate for nonlinear effects in conjunction with a first principles model) has not been seen previously in the literature.

This research introduces neural networks (NN) as a novel approach towards aiding biocomposite materials processing. At its core, the aim of the research was to investigate NN usage as a tool for advancing the field of biocomposites. Empirical data was generated for compression-molded flax fiber and High Density Polyethylene (HDPE) matrix based biocomposite materials. In an attempt to create the NN model, tensile strength, impact strength, hardness, bending strength, and density were provided to the NN as inputs. These inputs were processed through multiple layers of the NN, and contributed to the prediction of the composition (fiber loading percentage) and operating parameter (pressure in MPa) as output. In précis, NNs use was investigated to predict composition and operational parameter for biocomposites production when the desired mechanical properties of the biocomposites were available. Flax (Linum usitatissimum) fiber biocomposite boards were manufactured using chemically pretreated flax fiber and high density polyethylene (HDPE). After extensive preprocessing (combing and size reduction to 2 mm particles) and pretreatment regimen - flax fiber was mixed with HDPE and extruded using a laboratory scale single screw extruder. Extrudates generated from the extruder were again ground to 2 mm particles. Ground extrudates from different sample sets were exposed to a compression molding unit. The mold was put under two sets of pressures, (variable operating parameters) for all individual fiber loading. These boards were used to determine the mechanical properties tensile force, impact force, hardness, bending, and density. For verification and analysis of the mechanical properties, Microsoft Office Excel and a statistical software package SAS were used. After verification five different multilayer neural networks, i.e., cascade forward neural network, feedforward backpropagation neural network, neural unit (single layer, single neuron), feedforward time delay neural network and NARX, were trained and evaluated for performance. Ultimately, the feedforward backpropagation NN (FFBPNN) was selected as the most efficient. After rigorous testing, the FFBPNN trained by the TRAINSCG algorithm (Matlab ®) was selected to generate prediction results that were the most suitable, fast and accurate. Once the selection and training of the NN architecture was complete, biocomposite materials prediction was performed. From 9 separate input sets, NNs provided overall prediction error between 2 - and 4%. This was the same amount of error that was observed in the training of the neural network. It was concluded that the neural network approach for the experimental design and operational conditions were satisfied.

Taiwan is often threaten by typhoon during summer and autumn. The surges brought by theses typhoons not only cause human lives in danger, but also cause severe floods in coastal area. Storm surge prediction remains still a complex coastal engineering problem to solve since lots of parameters may affect the predictions. The purpose of this study is to predict storm surges using an Artificial Neural Network (ANN). A non-linear hidden-layer forward feeding neural network using back-propagation learning algorithms was developed. The study included a detailed analysis the factors may affect the predictions. The factors were obtained from the formulation of storm surge discrepancies after Horikawa (1987). Storm surge behaviors may vary from different geographical locations and weather conditions. A correlation analysis of the parameters was carried out first to pick up those factors shown high correlations as input parameters for establishing the typhoon surge predictions. The applications started with collecting tide and meteorological data (wind speed, wind direction and pressure) of Dapeng Bay and Kaohsiung harbor. A harmonic analysis was utilized to identify surge deviations. The surge deviation recorded at Dapeng Bay was found higher then Kaohsiung harbor for the same typhoon events. Correlation analysis has shown positive correlations between wind field, both wind speed and direction, and the associated storm surge deviations at Dapeng Bay. Correlation coefficients (CC) 0.6702 and 0.58 were found respectively. The variation of atmospheric pressure during typhoons is found with positive correlation too (i.e. CC=0.3626). Whereas the analysis has shown that the surges at Kaohsiung harbor were only sensitive to wind speed (CC=0.3723), while the correlation coefficients of the wind direction (CC=-0.1559) and atmospheric pressure (CC= -0.0337) are low. The wind direction, wind speed and atmospheric pressure variation were then used as input parameters for the training and predictions. An optimum network structure was defined using the Dapeng Bay data. The best results were obtained by using wind speed, wind direction and pressure variation as input parameters. The ANN model can predict the surge deviation better if the empirical mode decomposition (EMD) method was used for training.

SAS has become an increasingly important public-health problem in recent years. It can adversely affect neurocognitive, cardiovascular, respiratory diseases and can also cause behavior disorder. Moreover, up to 90% of these cases are obstructive sleep apnea (OSA). Therefore, the study of how to diagnose, detect and treat OSA is becoming a significant issue, both academically and medically. Polysomnography can monitor the OSA with relatively fewer invasive techniques. However, polysomnography-based sleep studies are expensive and time-consuming because they require overnight evaluation in sleep laboratories with dedicated systems and attending personnel. Therefore, to improve such inconveniences, one needs to develop a simplified method to diagnose the OSA, so that the OSA can be detected with less time and reduced financial costs. Since currently there seems to be no OSA detection technique available in Taiwan, the goal of this work is to develop a reliable OSA diagnostic algorithm. In particular, via signal processing, feature extraction and artificial intelligence, this thesis describes an on-line ECG-based OSA diagnostic system. It is hoped that with such a system the OSA can be detected efficiently and accurately.

Many parts used by the automobile manufacturers are provided by outside suppliers. Hence, the chain between the automobile manufacturers and their suppliers has been considered very important for the purchasing department of an automobile factory. Finding qualified suppliers that can meet the demands of the automobile manufacturers is thus an important issue. With the application of neural networks, this thesis develops an approach to help determining the qualification of the suppliers. By using data of the known qualified and unqualified suppliers and by setting a number of features to characterize the capability of the suppliers, neural networks are trained to determine the qualification of the suppliers. In training the neural networks, the features are incrementally removed until optimal classification accuracy is reached. It is hoped that this system can become an effective decision-supporting system in screening the potential suppliers for the automobile manufacturers.

Application of Image inpainting ranges from object removal, photo restoration, scratch removal, and so on. In this thesis, we will propose a modified multi-scale method and learning-based method using artificial neural networks for image inpainting. Multi-scale inpainting method combines image segmentation, contour estimation, and exemplar-based inpainting. The main goal of image segmentation is to separate image to several homogeneous regions outside the target region. After image segmentation, we use contour estimation to estimate curves inside the target region to partition the whole image into several different regions. Then we fill those different regions inside the target region separately by exemplar-based inpainting method. The exemplar-based technique fills the target region via the texture synthesis and filling order of exemplary patches. Exemplary patches are found near target region and the filling order is determined by isophote and densities of exemplary patches. Learning-based inpainting is a novel technique. This technique combines machine learning and the concept of filling order. We use artificial neural networks to learn the structure and texture surrounding the target region. After training, we fill the target region according to the filling order. From our simulation results, very good results can be obtained for removing large-size objects by using the proposed multi-scale method, and for removing medium-size objects of gray images.