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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Synaptic dynamics and neuronal integration in the cricket cercal sensory system

Hill, Andrew Allen 01 January 1996 (has links)
The function of a neural network is dependent on more than the static patterns of connections between neurons. For example, in response to time-varying stimuli in the environment, sensory neurons are activated in specific temporal patterns. Thus, to extract relevant information the neurons of a neural network must have the appropriate temporal properties. We have studied the dynamic properties of the synapses and of action potential generation in the cricket cercal system, a system that detects low-frequency air disturbances. First, we show that the electrotonic properties of the interneurons in the cricket cercal sensory system are preserved during postembyronic development, thereby maintaining their frequency filtering properties. Also, we found that the frequency of the spikes in the interneurons is linearly related to the level of injected current and that the interneurons show very little spike adaptation. Last, we combine a biophysical model of the spiking mechanism of an interneuron with the known temporal dynamics of the synaptic input in a computer simulation. We found that the rate of synaptic depression, a function of the fractional release rate of the sensory neuron synapses, is the most important factor in determining the temporal response of the interneurons to sound stimuli. In contrast, synaptic facilitation had a relatively weak effect on the temporal properties of the interneurons.
2

Characterization ofstalled, a grooming mutant that interacts with laminin

Reddy, Suman 01 January 1998 (has links)
A molecular and genetic dissection of the stalled grooming mutant was performed to characterize a gene which may be necessary for the assembly of the pupal and adult nervous system of Drosophila melanogaster. To determine whether the grooming deficit observed in stalled resulted from disrupting the development of the sensory nervous system, afferent projections were examined in stalled using two P (Gal4) lines as markers of subsets of femoral chordotonal neurons. These lines were isolated in a screen of P (Gal4) enhancer trap lines to identify those which label distinct subsets of sensory neurons and their afferent projections in the pupal and adult nervous system (Chapter 1). In addition, afferent projections from the posterior tactile neurons were examined in stalled mutants using the en-lacZ marker line. Because the stalled P element insertion site was mapped to the 67C region of the third chromosome (as was the laminin $\gamma$ gene), it was of interest to determine whether stalled had identified $laminin\ \gamma$ since it has been demonstrated that the laminin plays roles during neuronal development. It was possible that stalled would interact with $laminin\ \alpha$ as a result of identifying the $laminin\ \gamma$ gene. Therefore, a genetic analysis was performed by combining stalled with the $laminin\ \alpha$ mutant and examining projections from different classes of sensory receptors in the single and double mutants. It was found that the interaction with stalled and $laminin\ \alpha$ was required for the proper development of at least two classes of sensory receptors (Chapter 3). Therefore, molecular characterization of stalled was performed to determine if stalled had identified the $laminin\ \gamma$ gene. Northern analysis demonstrated that the expression of $laminin\ \gamma$ was decreased in stalled and the $stalled\sp{e71}$ hypomorph. However, mapping experiments demonstrated that the stalled P element insertion site was 20-60 kilobases downstream of $laminin\ \gamma$. Taken together, these results suggest that stalled has identified an enhancer of the $laminin\ \gamma$ gene such that the amount of the laminin heterotrimeric complex is reduced in the $laminin\alpha;stld$ double mutants. This results in significant effects on the development of at least two distinct classes of sensory afferent projections.

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