Thesis (Ph. D.)--University of Wisconsin--Madison, 1976. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Bibliographical references.

University, Diss., 2004--München.

Universiẗat, Diss., 2004--München.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1965. / Typescript. Vita. Description based on print version record. Includes bibliographical references (leaves 177-182).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1967. / Typescript. Two re-prints of journal articles co-authored by Michael S. Kappy and others bound in at end. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Universiẗat, Diss., 2002--München.

München, Univ., Diss., 2004

Thesis (Ph. D.)--University of Canterbury, 2008. / Typescript (photocopy). Two offprints bound in the back. Includes bibliographical references (leaves 142-162). Also available via the World Wide Web.

The 8.6 kb kalilo linear mitochondrial plasmid of Neurospora intermedia was found to give rise to multiple transcripts of 8.6, 4.4, 4.0, 1.3, 1.2, and 0.9 kb. A transcription map has been generated which shows similarities to other linear plasmids. These transcripts are all transcribed from a single, unique promoter sequence reiterated near the ends of the terminal inverted repeats of the linear plasmid. The transcripts are not processed, but instead utilize optional transcription stop sites. An analysis of sub-cellular RNA fractions has confirmed the mitochondrial location of kalilo transcription. The strong association of kalilo-specific RNA with rRNA to yield RNA artifacts is reported. Kalilo-specific RNA appears to be selectively unstable in affected strains of N. intermedia; this may be a general consequence of linear plasmid RNA. The 5' RNA start site was determined by primer extension and RNA sequencing. The sequence in this region does not show homology to any known mitochondrial, plasmid, nor nuclear promoter, and may constitute a novel element. The transcription start site shares homology with the terminus of the linear plasmid, and marks the end of a long series of direct repeats; therefore, the plasmid RNA polymerase may be bifunctional, it may recognize sequences at the ends of the plasmid as well as at the promoter. The analysis of the insertional behaviour of the linear mitochondrial plasmid was studied in parallel subculture series of the organism. It was determined that insertion, per se is not the event required to kill the organism. Generation of inserts of kalilo in the mtDNA is necessary, but not sufficient, for death to occur in all cases. An analysis of insertion sites has found one new site and good agreement with previously published locations. Insertion does not always appear to be random, so cultures may inherit undetectable amounts of mtlS-kalDNA. The analysis of insertion sites in one strain has suggested a novel possible structure for the mtDNA. / Medicine, Faculty of / Medical Genetics, Department of / Graduate

A survey of natural isolates of N. intermedia revealed five variants with respect to growth phenotype. These variants showed stop-start behavior in growth tubes, sometimes never attaining the full tube length. These strains are mainly.female sterile. The stop-start phenotype was not transmitted via the male parent in any cross. In two crosses, maternal transmission was demonstrated. One strain was investigated for the presence of virus-like particles, but none were found. Cytochrome spectra show a deficiency of cytochromes a. and/or b_ relative to the amount of c. In respiration studies, the one strain tested proved to be cyanide resistant and salicyl hydroxamic acid sensitive. These characteristics are also found in extranuclear mutants of N. ovassa. However, the analogy no longer holds at the mitochondrial ribosome level. An analysis of four of the strains disclosed that three are large subunit deficient. Only small subunit deficient strains have been found among the cytoplasmic mutants of N. ovassa. Restriction enzyme analysis was also carried out on the variants. It was found that they possess additional mitochondrial DNA compared to normal N. intermedia. One Eco RI fragment of M.W. 2.4 x 10 was found to be common to all variants tested, but is not found in the normal strains. There were other DNA differences which were unique to each abnormal strain. These findings suggest that the basis for the abnormal phenotypes .is some heritable factor associated with the mitochondria. / Science, Faculty of / Botany, Department of / Graduate