Multiple-Input-Multiple-Output (MIMO) antenna technology refers to an antenna with multiple radiators at both transmitter and receiver ends. It is designed to increase the data rate in wireless communication systems by achieving multiple channels occupying the same bandwidth in a multipath environment. The main drawback associated with this technology is the coupling between the radiating elements. A MIMO antenna system merely acts as an antenna array if the coupling between the radiating elements is high. For this reason, strong decoupling between the radiating elements should be achieved, in order to utilize the benefits of MIMO technology. The main objectives of this thesis are to investigate and implement several printed MIMO antenna geometries with integrated decoupling approaches for WLAN, WiMAX, and 5G applications. The characteristics of MIMO antenna performance have been reported in terms of scattering parameters, envelope correlation coefficient (ECC), total active reflection coefficient (TARC), channel capacity loss (CCL), diversity gain (DG), antenna efficiency, antenna peak gain and antenna radiation patterns. Three new 2×2 MIMO array antennas are proposed, covering dual and multiple spectrum bandwidths for WLAN (2.4/5.2/5.8 GHz) and WiMAX (3.5 GHz) applications. These designs employ a combination of DGS and neutralization line methods to reduce the coupling caused by the surface current in the ground plane and between the radiating antenna elements. The minimum achieved isolation between the MIMO antennas is found to be better than 15 dB and in some bands exceeds 30 dB. The matching impedance is improved and the correlation coefficient values achieved for all three antennas are very low. In addition, the diversity gains over all spectrum bands are very close to the ideal value (DG = 10 dB). The forth proposed MIMO antenna is a compact dual-band MIMO antenna operating at WLAN bands (2.4/5.2/5.8 GHz). The antenna structure consists of two concentric double square rings radiating elements printed symmetrically. A new method is applied which combines the defected ground structure (DGS) decoupling method with five parasitic elements to reduce the coupling between the radiating antennas in the two required bands. A metamaterial-based isolation enhancement structure is investigated in the fifth proposed MIMO antenna design. This MIMO antenna consists of two dual-band arc-shaped radiating elements working in WLAN and Sub-6 GHz 5th generation (5G) bands. The antenna placement and orientation decoupling method is applied to improve the isolation in the second band while four split-ring resonators (SRRs) are added between the radiating elements to enhance the isolation in the first band. All the designs presented in this thesis have been fabricated and measured, with the simulated and measured results agreeing well in most cases. / Higher Committee for Education Development in Iraq (HCED)