Mode of access: World Wide Web. / Thesis (Ph. D.)--University of Hawaii at Manoa, 2005. / Includes bibliographical references. / Electronic reproduction. / Also available by subscription via World Wide Web / xv, 222 leaves, bound col. ill 29 cm

Thesis (M.A.)--University of Missouri-Columbia, 2007. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on November 6, 2007) Includes bibliographical references.

Thesis (M.S.)--University of Hawaii at Manoa, 2006. / Includes bibliographical references (leaves 50-56). / viii, 56 leaves, bound ill. (some col.) 29 cm

Köln, Univ., Diss., 2002. / Computerdatei im Fernzugriff.

Köln, Universiẗat, Diss., 2002.

Thesis (Ph. D.)--University of Missouri-Columbia, 2007. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on September 25, 2007) Vita. Includes bibliographical references.

No description available.

La moléculture végétale a connu un essor considérable ces dernières années, permis parnombre d’avantages pratiques associés aux plantes incluant une mise à l’échelle aisée pour la production de vaccins ou d’anticorps thérapeutiques en grandes quantités. De nombreuses études ont été réalisées, ces dernières années, pour optimiser les rendements en protéines d’intérêt médical chez Nicotiana benthamiana, une cousine sauvage du tabac.Ces études ont été menées, toutefois, sans tenir compte de la teneur foliaire en protéines endogènes, souvent considérées comme des contaminants majeurs au moment de la purification des protéines d’intérêt. Dans ce contexte, notre principal objectif de recherche pour cette étude a été d’évaluer l’influence de traitements culturaux variés aussi bien sur le rendement total en protéine recombinante dans des plants agroinfiltrés de N. benthamianaque sur la teneur relative en ribulose-1,5-bisphosphate carboxylase/oxygénase (RuBisCO),principal contaminant endogène du tissu foliaire. Nos résultats confirment un impact souvent significatif des conditions culturales sur le rendement total en protéine recombinante dans la plante, mais peu d’effets sur la part relative en RuBisCO dans le tissu foliaire. / Plant molecular farming has grown considerably in recent years, thanks to a number of benefits associated with plants including a convenient scaling-up of production settings that allow for the production of large quantities of vaccines or therapeutic antibodies. Numerous studies have been conducted in recent years to optimize the yields of medically valuable recombinant proteins in the wild tobacco relative Nicotiana benthamiana. Most of these studies, however, have not considered the host plant endogenous proteins, which oftenrepresent major contaminants during the downstream purification of recombinant proteins.In this context, our main research objective for this project has been to evaluate the relative impacts of cultural conditions on recombinant protein yield and relative content of ribulose1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the major endogenous protein contaminant in leaf tissue. Our results confirm the influence of most cultural conditions on total recombinant protein yield, but a negligible effect of these conditions on the relative amount of RuBisCO in leaf tissue.

Thesis (Ph. D.)--University of Hawaii at Manoa, 2005. / Includes bibliographical references.

No description available.