Accelerating rates of urbanization create novel compilations of species in urban ecosystems which experience unprecedented proximity to large human populations. Although an integral part of these urban ecosystems, mammals are difficult to study in urbanized areas. However, questions regarding urban mammalian ecology, such as the maintenance or disruption of predator-prey relationships, are vital to understanding what impacts anthropogenic factors may exert on the functions of urban ecosystems. We show that non-trapping hair equipment can effectively collect hair samples from two mammal groups – small mammals and mesopredators – in urban areas for the purpose of addressing ecological questions. We deployed non-trapping hair collection equipment at 16 different parks, preserves, and nature areas along the urbanization gradient of Philadelphia, Pennsylvania. We collected a total of 354 small mammal and 41 mesopredator hair samples. Of these samples, 58 small mammal samples and 14 mesopredator samples were of adequate weight for stable isotope analysis. We analyzed relationships between δ15N and δ13C values from each species and percent impervious surface (our proxy for urbanization) to explore potential shifts in mammal diets. Our results indicated a decoupling of predator-prey relationships between mesopredators and small mammals, particularly in urban spaces and suggested that mesopredators may be relying upon anthropogenic food subsidies. Our methodology was not only effective in addressing an ecological question, but it also provided a unique opportunity to connect with community members through QR code labels attached to our field equipment. Non-trapping hair collection methods offer a viable way to collect useful samples in urban areas while also fostering connections between community members and field research occurring in their neighborhoods. / Biology