published_or_final_version / abstract / Paediatrics and Adolescent Medicine / Master / Master of Philosophy

Thesis (M. Phil.)--University of Hong Kong, 2008. / Also available in print.

This thesis describes the development of an immunofluorescent technique and its application to a cytochemical study of the intracellular distribution of a basic protein component which satisfies several criteria used to identify histone. The technique was developed in three steps: 1. Production of Antibody The method of nuclear protein extraction, the preparation of the inoculum, the immunisation procedure and the species of antigen donor and recipient were all considered contributory of the successful production of antibody. 2. Analyses of Antigen and Antibody Electrophoretic and chromatographic techniques were employed to characterise the nuclear protein antigens. The antiserum was purified by immunological absorption and chromatography. The antigen-antibody responses were studied using electrophoretic and immunodiffusion techniques, both singly and in combination. 3. Application to Cell Preparations Fluorescent antibody provided a protein tracer with which to demonstrate the precise cytological location of analytically defined nuclear proteins. The antibody was produced in the chicken against basic protein from calf thymus nuclei. It was specific for nuclei and chromosomes but was neither organ nor species specific. / Medicine, Faculty of / Medical Genetics, Department of / Graduate

Thesis (M.S.)--Florida State University, 2005. / Advisor: Dr. Hong Li, Florida State University, College of Arts and Sciences, Dept. of Chemistry and Biochemistry. Title and description from dissertation home page (viewed Sept. 16, 2005). Document formatted into pages; contains vi, 45 pages. Includes bibliographical references.

No description available.

No description available.

Thesis (Ph. D.)--Ohio State University, 2004. / Title from first page of PDF file. Document formatted into pages; contains xvii, 287 p.; also includes graphics (some col.). Includes bibliographical references (p. 282-287). Available online via OhioLINK's ETD Center

In these studies, a procedure which allowed the in vivo labeling and detection of poly(ADP-ribose) was combined with nuclear fractionation techniques to analyze the nuclear distribution of ADP-ribose polymers. The results from these studies suggest the occurrence of poly(ADP-ribose) metabolism in two compartments of chromatin; one that is nuclear matrix-associated and one that is not. The biological significance of this compartmentalization is conceptualization in a model. This model postulates that, under some physiological conditions, poly(ADP-ribose) metabolism accomplishes the reversible targeting of specific regions of chromatin to the nuclear matrix domain by modulating DNA-protein and or protein-protein interactions.

published_or_final_version / Paediatrics and Adolescent Medicine / Master / Master of Philosophy

Tam, Yeuk Fei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 63-74). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts also in Chinese.