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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Sequenciamento do baculovírus que infecta a broca-da-cana-de-açúcar Diatraea saccharalis. / Sequencing of baculovirus that infects sugar cane borer Diatraea saccharalis.

Pereira, Bruna Tibirica 23 January 2014 (has links)
Baculovírus são vírus específicos de inseto que infectam principalmente membros da ordem Lepidoptera. Diatraea saccharalis granulovírus (DsGV) foi isolado de larvas de Diatraea saccharalis (Lepidoptera: Crambidae), a broca-da-cana-de-açúcar, um dos insetos-praga de maior importância na cultura de cana-de-açúcar no Brasil. O genoma completo de DsGV foi obtido através do sequenciamento 454 (Roche). O genoma de DsGV apresentou 98.463 pb e potencialmente codifica 116 genes. Foram identificados os 37 genes conservados em todos os baculovírus, 19 genes específicos de betabaculovírus e 17 genes únicos. DsGV é o primeiro betabaculovírus que possui o gene gp64, que codifica uma proteína de fusão, originalmente encontrado apenas em alfabaculovírus do grupo I. A análise filogenética utilizando a concatenação das sequências deduzidas de aminoácidos de 30 genes conservados em 61 baculovírus totalmente sequenciados sugere que DsGV está inserido no clado b do grupo dos betabaculovírus e parece estar mais estritamente relacionado a 5 GVs (ChocGV, PiraGV, ClanGV, CpGV e CrleGV). / Baculoviruses are insect specific viruses that infect mainly members of the Order Lepidoptera. Diatraea saccharalis granulovirus (DsGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pest of the sugar cane culture in Brazil. The genome of DsGV was obtained by the 454 sequencing system (Roche). Our results showed that the nucleotide sequence of the DsGV genome is 98.463 bp in length and potentially encodes 116 putative genes. It contains the 37 baculovirus core genes, a set of 19 betabaculovirus-specific genes and 17 putative DsGV genes were not found in any genome of the baculoviruses sequenced up to the present. DsGV is the first betabaculovirus sequenced so far that has the gp64 envelope fusion protein gene, originally found only in alphabaculovirus group I. Phylogenetic analysis performed with concatamers of 30 conserved proteins from 61 fully sequenced baculovirus genomes suggests that DsGV is a member of clade b of the betabaculovirus and seems to be closer to 5 GVs (ChocGV, PiraGV, ClanGV, CpGV e CrleGV).
2

Sequenciamento do baculovírus que infecta a broca-da-cana-de-açúcar Diatraea saccharalis. / Sequencing of baculovirus that infects sugar cane borer Diatraea saccharalis.

Bruna Tibirica Pereira 23 January 2014 (has links)
Baculovírus são vírus específicos de inseto que infectam principalmente membros da ordem Lepidoptera. Diatraea saccharalis granulovírus (DsGV) foi isolado de larvas de Diatraea saccharalis (Lepidoptera: Crambidae), a broca-da-cana-de-açúcar, um dos insetos-praga de maior importância na cultura de cana-de-açúcar no Brasil. O genoma completo de DsGV foi obtido através do sequenciamento 454 (Roche). O genoma de DsGV apresentou 98.463 pb e potencialmente codifica 116 genes. Foram identificados os 37 genes conservados em todos os baculovírus, 19 genes específicos de betabaculovírus e 17 genes únicos. DsGV é o primeiro betabaculovírus que possui o gene gp64, que codifica uma proteína de fusão, originalmente encontrado apenas em alfabaculovírus do grupo I. A análise filogenética utilizando a concatenação das sequências deduzidas de aminoácidos de 30 genes conservados em 61 baculovírus totalmente sequenciados sugere que DsGV está inserido no clado b do grupo dos betabaculovírus e parece estar mais estritamente relacionado a 5 GVs (ChocGV, PiraGV, ClanGV, CpGV e CrleGV). / Baculoviruses are insect specific viruses that infect mainly members of the Order Lepidoptera. Diatraea saccharalis granulovirus (DsGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pest of the sugar cane culture in Brazil. The genome of DsGV was obtained by the 454 sequencing system (Roche). Our results showed that the nucleotide sequence of the DsGV genome is 98.463 bp in length and potentially encodes 116 putative genes. It contains the 37 baculovirus core genes, a set of 19 betabaculovirus-specific genes and 17 putative DsGV genes were not found in any genome of the baculoviruses sequenced up to the present. DsGV is the first betabaculovirus sequenced so far that has the gp64 envelope fusion protein gene, originally found only in alphabaculovirus group I. Phylogenetic analysis performed with concatamers of 30 conserved proteins from 61 fully sequenced baculovirus genomes suggests that DsGV is a member of clade b of the betabaculovirus and seems to be closer to 5 GVs (ChocGV, PiraGV, ClanGV, CpGV e CrleGV).

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