Dietary carbohydrate during pregnancy is essential but whether this requirement is specific to glucose or if fructose could substitute for glucose in the diet of pregnant rat dams was investigated. It was concluded that the carbohydrate requirement for the rat during pregnancy is not specific to glucose and the level, not the type, of carbohydrate was critical. The potential toxicity of high fructose diets was also investigated. Dams fed high fructose had significantly higher liver weights than dams fed high glucose while other toxic indicators were not affected. A third aspect was the comparison of isocaloric, low carbohydrate diets containing different sources of 4% glucose equivalents: glucose, fructose or lipid-glycerol. Fructose and lipid-glycerol were not adequate substitutes for glucose. The measurement of amniotic fluid glucose, which increased as either dietary glucose or fructose increased in the maternal diet may be a new, accessible nutritional indicator of carbohydrate status.

Despite the acknowledged importance of nutritional support for COPD patients, it is difficult to accomplish in acutely stressed individuals. A randomized trial of nutritional supplementation during an acute exacerbation was carried out in 16 hospitalized patients for a 2 week period. Six control patients consumed a standard diet supplying 1,951 $ pm$ 130 (mean $ pm$ SEM) kcal and 80 $ pm$ 6 g protein/d, while ten treatment patients, in addition to the usual diet received oral supplements (Ensure) or snacks, resulting in an intake of 2,516 $ pm$ 129 kcal (p = 0.012) and 99 $ pm$ 6 g protein/d (p = 0.059). Although the treatment subjects improved their intake over the control group, no significant improvement in nutritional status occurred in either group. / Forced vital capacity (FVC % predicted) improved significantly over the study period in treated vs control subjects (+11.10 $ pm$ 4.63 vs $-$4.50 $ pm$ 2.14; p = 0.026). Nitrogen balances were calculated for 9 subjects, and all were in negative balance ($-$8.42 $ pm$ 1.74 g nitrogen/d) with no difference between groups. / Because of the high doses of methylprednisolone administered (69.6 $ pm$ 8.3 mg/d), and their known catabolic effects, we examined whether the dose affected nitrogen balance and muscle strength. Both nitrogen balance (r = $-$0.73; p = 0.025) and grip strength (r = $-$0.76; p $<$ 0.001) worsened with higher doses of steroids. The catabolic process may have resulted from elevated energy requirements, inadequate intake of protein and energy or been induced by high doses of steroids. / Hospitalized COPD patients are highly stressed and catabolic, and the means to preventing protein wasting during an acute exacerbation of their disease remains to be established. (Abstract shortened by UMI.)

Dietary factors such as the consumption of tryptophan, the precursor of serotonin, or sugar, may play an important role in influencing alcohol-induced impairment. Study I used an amino acid diet to manipulate plasma tryptophan in male social drinkers in a 3 (supplemented, balanced, and depleted tryptophan) x 3 (alcohol, placebo, sober) design with 12 subjects per cell. A variety of mood, memory, and psychomotor performance measures were taken at baseline, five hours after amino acid ingestion, and after alcohol consumption. Strong alcohol effects were produced but no tryptophan, nor alcohol-tryptophan interaction effects were found. / Study II examined the combined effects of sucrose and alcohol in a 3 (100 g sugar, 35 g sugar, 0 g sugar) x 3 (alcohol, placebo, sober) design with 15 subjects per cell. Subjects were tested on tasks similar to those used in Study I at a variety of times following intoxication (i.e., 0.5 hours, 1.5 h, 3.5 h) in order to examine effects when blood glucose peaked as well as at a point when hypoglycemic rebound can occur in some subjects. A strong alcohol x sugar interaction was seen 0.5 h after drinking, with high doses of sugar attenuating intoxication without influencing blood alcohol levels. No hypoglycemia was produced after 3.5 h. / Study III replicated Study II using a simpler design which controlled for the possible effects of aspartame (the placebo sweetener used in Study II). A variety of gastric or central mechanisms, which can account for the finding that sucrose can reduce the intoxicating effects of ethanol, are discussed.

Multiple sclerosis (MS) is a neurodegenerative disease of uncertain etiology and pathology, affected by genetic and environmental factors. Nutrition may be one of these factors. This study used one validated 80-item food frequency questionnaire and one 24-hour recall to assess dietary intake, anthropometric and lifestyle practices, and self-perceived impacts of food in 36 adults with MS. Although only 3 persons followed special diets for MS, 28 indicated food influenced the way they felt, and 17 thought food directly impacted their MS. Over 50% of participants were identified as potential underreporters of energy. Low intakes in grains, dairy, and fruits and vegetables were commonly observed. The FFQ and 24-hour recalls estimated different mean sample intakes of all nutrients; these differences were significant for carbohydrates, water, iron, magnesium, riboflavin, niacin, and omega-3. Both tools identified mean intakes below suggested AI levels for fibre, calcium, vitamin D, omega-3 and omega-6, and above the UL for sodium. Supplements were used by 72% of participants, and increased estimated nutrient intakes above the EAR or the UL for some nutrients in some individuals.

Dietary linoleate enhances the development of mammary tumors while dietary stearate has a retarding effect. Since linoleate is a precursor of prostaglandin E2 and elevated levels of prostaglandin E2 have been shown to be associated with tumor development, levels of dietary linoleate directly influence the levels of prostaglandin E2 available for tumor development. The development of new tumor tissue also involves a high demand for fatty acids to be incorporated into cell membranes and this demand is for specific types of fatty acids to maintain the required membrane fluidity.Several enzymes play key roles in the synthesis and desaturation of the fatty acids which are converted to prostaglandins and are synthesized for membrane incorporation. Among these are fatty acid synthetase and linoloeyl coenzyme A desaturase. This study examined the activity of these two enzymes in liver tissue of mice maintained on test diets containing varying proportions of the fatty acids stearate and linoleate.Animals fed a high fat (15% of total) diet containing 13.1% stearate were found to have higher linoloeyl coenzyme A deeaturaae activity than animals fed a low fat diet or a high fat diet containing 11.3% linoleate diets. Animals fed the high fat, high stearate diet were found to have a higher fatty acid synthetase activity than animals fed either a low fat diet or the high fat, high linoleate diet.

No description available.

This thesis examined aspects of production and utilization of acetate in humans via measurements of plasma concentrations in different circumstances with particular attention to changes in diabetes. Circulating plasma acetate was measured by a modified acetate kinase-based enzymatic spectrophotometric method with adequate sensitivity and specificity for levels encountered in human plasma. Fasting plasma acetate was increased in diabetics and correlated with glucose and indices of glucose disposal. Levels increased further when they were fed different high- fibre diets. The rise in acetate levels after lactulose ingestion correlated with changing breath hydrogen excretion in subjects with suspected malabsorption. Plasma acetate levels increased during fat infusion, and conversely, fell with suppression of fatty acid levels during euglycaemic clamping. Insulin appeared to promote acetate production from glucose by enhancing glycolysis and acetyl CoA availability, although its activity in reducing lipolysis had an opposite effect. The hepatic formation of acetate from ethanol did not appear influenced by prior chlorpropamide intake. Glucose tolerance was unaffected by a 150mmol/hr acetate load, but acetate tolerance was impaired when glucose was simultaneously available. Adipose tissue lipolysis was suppressed during acetate infusions as evident from reduced levels of glycerol and non-esterifled fatty acids. Blood 'ketone body' levels were increased, suggesting direct conversion from acetate. Possibly as a result, fat oxidation assessed from gaseous exchange, was reduced with infused acetate. Acetate utilization was impaired in diabetic patients from higher fasting plasma levels and slower metabolic clearance. The defect in diabetes was probably due to both over-production and under-utilization, and could be related to the enhanced lipolysis, hyperglycaemia and a reportedly reduced hepatic activity of acetyl CoA synthetase. It was concluded that acetate is derived from both colonic fermentation and endogenous catabolism of glucose and fatty acids and appears rapidly metabolisable in humans. Some areas of further interest in human acetate metabolism were highlighted.

Orientador: Sergio Alberto Rupp de Paiva / Resumo: A gravidez é uma fase do ciclo de vida em que a nutrição desempenha um papel muito importante, uma vez que esta influência o produto da concepção. O objetivo deste estudo foi descrever o estado nutricional de gestantes do município de Botucatu que se localiza na região centro sul do estado de São Paulo, distando aproximadamente 230km da Capital. A população é predominantemente urbana. Foram estudadas 496 gestantes com idade de 13-43 anos submetidas a entrevista para obtenção de dados socioeconômicos, demográficos e dietéticos, e coleta de sangue. Dentre as mulheres 83% eram brancas. 30,5% de adolescente, a renda mostrou que 69% estava abaixo da linha de pobreza, a escolaridade mostrou 63% era baixa, 79% casada, 25% eram fumantes, 41% eram primigestas e 12% apresentaram positividade para o exame parasitológico. As medianas para as variáveis antropométricas (percentagem de gestantes abaixo do ponto de corte) foram para: peso pré gestacional-56,0 kg;(20), peso gestacional-58,1 kg (15); estatura -158 cm(9), índice de massa corpórea pré gestacional-22,0 kg/m2 (18,7), circunferência do braço-270 mm (8,0), prega tricipital-8,6 mm (4,0).O IMC, ainda detectou 17,5% acima da normalidade. O ganho de peso semanal foi 180 g, mostrando valores maiores nas gestantes com IMC abaixo da normalidade. As medianas da ingestão de nutrientes (percentagem de gestantes que ingerem menos que 100% do RDA) foram para : energia 2186 Kcal (73), proteína 64,5 g (42), vitamina A 647,6 mg (56), a tocoferol 4,3 mg (100), vitamina C 106,0 mg (30) e ferro 11,0 mg (99). Os exames bioquímicos mostraram as medianas (percentagem abaixo do ponto de corte): hemoglobina 13,0 g/dL (3),ferro 92mg/dL (13), saturação da transferrina... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Pregnancy is a life cycle where nutrition plays an important role once it influences the product of conception. This study aimed to describe nutritional status of pregnant women in Botucatu located in the southern part of the State of São Paulo, 230 km far from the capital (São Paulo City). Population is predominantly an urban one. Pregnant women (496) between 13 and 43 years old were studied and subjected to interviews to obtain social, economic, demographic, dietetic data a well as blood sampling. Within the women 83% were white, 30.5% adolescents and the income showed that 69% were down poverty line, education was low 63%; 79% were married, 25% were smokers, 41% were pregnant for the first time, and 12% were positive for parasitological exam. Medians and anthropometric variables (percentage of pregnant women below cut point) were: pre-gestational weight - 56.0 kg (20) gestational weight - 58.1 kg (15) height 158 cm (9) ; pre-gestational body mass index 22.0 kg/m2 (15.o); arm circumference 270 mm (8.0); tricipital fold 18.6 mm (4.0) . BMI also detected 15.5% above normality. Weekly weight gain was 180g, with higher values on below normal BMI pregnant women. Nutrients intake medians (percentage of pregnant women who intake less than 100 % of RDA) was: energy 2186 Kcal (73), protein 64.5g (42) , vitamin A 647.mg (56), a- tocopherol 4.3 mg (100), vitamin C 106.0 mg (30) and iron 11.0 mg (99). Biochemical examinations showed medians (percentage below cut point) : hemoglobin 13.0 g/dL (3), iron 92 μ/dL (13), transferrin saturation 30.0% (8), ferritine... (Complete abstract click electronic access below) / Mestre

Made available in DSpace on 2014-06-11T19:27:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2002Bitstream added on 2014-06-13T20:35:37Z : No. of bitstreams: 1 cassettari_ml_me_botfm.pdf: 612511 bytes, checksum: 91a08595afc714e2109c38cfafc15036 (MD5) / A gravidez é uma fase do ciclo de vida em que a nutrição desempenha um papel muito importante, uma vez que esta influência o produto da concepção. O objetivo deste estudo foi descrever o estado nutricional de gestantes do município de Botucatu que se localiza na região centro sul do estado de São Paulo, distando aproximadamente 230km da Capital. A população é predominantemente urbana. Foram estudadas 496 gestantes com idade de 13-43 anos submetidas a entrevista para obtenção de dados socioeconômicos, demográficos e dietéticos, e coleta de sangue. Dentre as mulheres 83% eram brancas. 30,5% de adolescente, a renda mostrou que 69% estava abaixo da linha de pobreza, a escolaridade mostrou 63% era baixa, 79% casada, 25% eram fumantes, 41% eram primigestas e 12% apresentaram positividade para o exame parasitológico. As medianas para as variáveis antropométricas (percentagem de gestantes abaixo do ponto de corte) foram para: peso pré gestacional-56,0 kg;(20), peso gestacional-58,1 kg (15); estatura -158 cm(9), índice de massa corpórea pré gestacional-22,0 kg/m2 (18,7), circunferência do braço-270 mm (8,0), prega tricipital-8,6 mm (4,0).O IMC, ainda detectou 17,5% acima da normalidade. O ganho de peso semanal foi 180 g, mostrando valores maiores nas gestantes com IMC abaixo da normalidade. As medianas da ingestão de nutrientes (percentagem de gestantes que ingerem menos que 100% do RDA) foram para : energia 2186 Kcal (73), proteína 64,5 g (42), vitamina A 647,6 mg (56), a tocoferol 4,3 mg (100), vitamina C 106,0 mg (30) e ferro 11,0 mg (99). Os exames bioquímicos mostraram as medianas (percentagem abaixo do ponto de corte): hemoglobina 13,0 g/dL (3),ferro 92mg/dL (13), saturação da transferrina... / Pregnancy is a life cycle where nutrition plays an important role once it influences the product of conception. This study aimed to describe nutritional status of pregnant women in Botucatu located in the southern part of the State of São Paulo, 230 km far from the capital (São Paulo City). Population is predominantly an urban one. Pregnant women (496) between 13 and 43 years old were studied and subjected to interviews to obtain social, economic, demographic, dietetic data a well as blood sampling. Within the women 83% were white, 30.5% adolescents and the income showed that 69% were down poverty line, education was low 63%; 79% were married, 25% were smokers, 41% were pregnant for the first time, and 12% were positive for parasitological exam. Medians and anthropometric variables (percentage of pregnant women below cut point) were: pre-gestational weight – 56.0 kg (20) gestational weight - 58.1 kg (15) height 158 cm (9) ; pre-gestational body mass index 22.0 kg/m2 (15.o); arm circumference 270 mm (8.0); tricipital fold 18.6 mm (4.0) . BMI also detected 15.5% above normality. Weekly weight gain was 180g, with higher values on below normal BMI pregnant women. Nutrients intake medians (percentage of pregnant women who intake less than 100 % of RDA) was: energy 2186 Kcal (73), protein 64.5g (42) , vitamin A 647.mg (56), a- tocopherol 4.3 mg (100), vitamin C 106.0 mg (30) and iron 11.0 mg (99). Biochemical examinations showed medians (percentage below cut point) : hemoglobin 13.0 g/dL (3), iron 92 μ/dL (13), transferrin saturation 30.0% (8), ferritine... (Complete abstract click electronic access below)

Acquired Immune Deficiency Syndrome (AIDS) and impaired or threatened nutritional status seem to be closely related. It is now known that AIDS results in many nutritional disorders including anorexia, vomiting, protein-energy malnutrition (PEM), nutrient deficiencies, and gastrointestinal, renal, and hepatic dysfunction (1-7, 8). Reversibly, nutritional status may also have an impact on the development of AIDS among HIV-infected people. Not all individuals who have tested antibody positive for the Human Immunodeficiency Virus (HIV) have developed AIDS or have even shown clinical symptoms (9, 10). A poor nutritional status, especially PEM, has a depressing effect on immunity which may predispose an individual to infection (11). It has been proposed that a qualitatively or quantitatively deficient diet could be among the factors precipitating the transition from HIV-positive to AIDS (12, 13). The interrelationship between nutrition and AIDS reveals the importance of having a multidisciplinary health care team approach to treatment (11), including having a registered dietitian on the medical team. With regards to alimentation, the main responsibility of a dietitian is to inform the public concerning sound nutritional practices and encourage healthy food habits (14). In individuals with inadequate nutritional behavior, a positive, long-term change has been seen when nutrition education tailored to specific physiological and emotional needs was provided along with psychological support through counseling (14). This has been the case for patients with various illnesses and may also be true in AIDS patients as well. Nutritional education specifically tailored for each AIDS patient could benefit the patient by improving the quality of life and preventing or minimizing weight loss and malnutrition (15-17). Also, it may influence the progression of the disease by delaying the onset of the most severe symptoms and increasing the efficacy of medical treatment (18, 19). Several studies have contributed to a dietary rationale for nutritional intervention in HIV-infected and AIDS patients (2, 4, 20-25). Prospective, randomized clinical research in AIDS patients have not yet been published to support this dietary rationale; however, isolated case reports show its suitability (3). Furthermore, only nutrition intervention as applied by a medical team in an institution or hospital has been evaluated. Research is lacking concerning the evaluation of nutritional education of either non-institutionalized or hospitalized groups of persons who are managing their own food choice and intake. This study compares nutrition knowledge and food intakes in HIV-infected individuals prior to and following nutrition education. It was anticipated that education would increase the knowledge of nutritional care of AIDS patients and lead to better implementation of nutrition education programs.