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Oligonucleotides applied in genomics, bioinformatics and development of molecular markers for rice and barleyLiu, Shaolin, 1968- January 2004 (has links)
A genome sequence can be conceptualized as a 'book' written with four nucleotide 'letters' in oligonucleotide (oligo) 'words'. These words can be used in genomics, bioinformatics and the development of molecular markers. The whole-genome sequence for rice (Oryza sativa L.) is almost finished and has been assembled into pseudomolecules. For barley ( Hordeum vulgare L.) expressed sequence tags (ESTs) have been assembled into 21,981 tentative consensus sequences (TCs). The availability of such sequence information provides opportunities to investigate oligo usage within and between genomes. For the first of three studies reported in this thesis, a C++ program was written to automatically design oligos that are conserved between two sets of sequence information. In silico mapping between rice coding sequences (CDS) and barley TCs indicated that oligos between 18 and 24 bp provide good specificity and sensitivity (83% and 86%, respectively, for 20mers). Conserved oligos used as PCR primers had a high (91%) success rate on barley lines. Sequencing of PCR products revealed conservation in exon sequence, size and order between barley and rice. Introns were not conserved in sequence but were relatively stable in size. Map locations of eight new markers in barley revealed both genome colinearity and rearrangements between barley and rice. The second study reported in this thesis examined word frequency within the rice genome. A non-random landscape composed of high-frequency and low-frequency zones was observed. Interestingly, high-frequency words seemed to be rice specific while single-copy words were gene specific and conserved across species. As in the first study, oligos of 12 bp or less were not specific, and 18 bp seemed to be a critical length for the specificity of oligos. The third study reported in this thesis involved the development of molecular markers for known genes using public sequence information. Six new polymorphic markers were d
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Fiber optic microsphere-based oligonucleotide arrays : new developments and applications /Epstein, Jason R. January 2004 (has links)
Thesis (Ph.D.)--Tufts University, 2004. / Adviser: David R. Walt. Submitted to the Dept. of Chemistry. Includes bibliographical references. Access restricted to members of the Tufts University community. Also available via the World Wide Web;
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Computational prediction of antisense oligonucleotides and siRNAs /Chalk, Alistair, January 2005 (has links)
Diss. (sammanfattning) Stockholm : Karolinska institutet, 2005. / Härtill 6 uppsatser.
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A solid-state NMR investigation of structure and dynamics in nucleosides and methylated DNA oligonucleotides /Geahigan, Karen Brigitte. January 1998 (has links)
Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [251]-261).
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An evaluation of extraction parameters and LCMS analysis of oligonucleotides from biological matricesFelts, Jennifer Elaine. January 2008 (has links) (PDF)
Thesis (M.S.)--University of North Carolina Wilmington, 2008. / Includes appendixes. Title from PDF title page (viewed May 27, 2009). Includes bibliographical references (p. 96).
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Development and validation of a DNA microarray for analysis of the Bradyrhizobium japonicum transcriptomeFranck, William L., Stacey, Gary, January 2009 (has links)
Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 24, 2010). The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Dissertation advisor: Dr. Gary Stacey. Vita. Includes bibliographical references.
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Nucleic acid based reagentless optical biosensorsRajendran, Manjula, Ellington, Andrew D., January 2004 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisor: Andrew D. Ellington. Vita. Includes bibliographical references. Also available from UMI.
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Synthesis and gene silencing activity of RNA duplexes containing 3'-deoxy-3'-thiothymidineIsaac, Siara Ruth, January 1900 (has links)
Written for the Dept. of Chemistry. Title from title page of PDF (viewed 2009/06/25). Includes bibliographical references.
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Part 1 controlling barriers to charge transfer in DNA : Part 2 : DNA-directed assembly of conducting oligomers /Güler, Gözde. January 2008 (has links)
Thesis (Ph. D.)--Chemistry and Biochemistry, Georgia Institute of Technology, 2009. / Committee Chair: Schuster, Gary; Committee Member: Barry, Bridgette; Committee Member: Collard, David; Committee Member: Tolbert, Laren; Committee Member: Wartell, Roger. Part of the SMARTech Electronic Thesis and Dissertation Collection.
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Examination of gas-phase conformations of oligonucleotides using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry /Robinson, Jessica Marie, January 1998 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 1998. / Vita. Includes bibliographical references (leaf 171). Available also in a digital version from Dissertation Abstracts.
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