Thesis (Ph. D.)--Ohio State University, 2003. / Title from first page of PDF file. Document formatted into pages; contains xxiv, 529 p.; also includes graphics Includes bibliographical references (p. 510-529). Available online via OhioLINK's ETD Center

This thesis describes the novel application of laser desorption ionization Fourier transform ion cyclotron resonance (l.d.i.-F.t.-i.c.r.) mass spectrometry for determining the sequence of underivatized oligosaccharides. As a starting point, four model oligosaccharides obtained from bacteriophage degradation of the corresponding bacterial capsular polysaccharides were analyzed. The experiments progressed from a linear pentasaccharide (Klebsiella K44), to a linear pentasaccharide containing an acid-labile pyruvic acid (Klebsiella K3), to a linear tetrasaccharide containing an acetamido hexose and an acid-labile neuraminic acid (E. coli K9), and finally to a "3+1" branched tetrasaccharide containing an lactic acid ether and a base-labile O-acetate (Klebsiella 22). The glycosidic bond and significant ring cleavage fragmentations observed in the mass spectra could be used for the sequencing of all three linear oligosaccharides. Furthermore, these unusual ring-cleaved fragments also provided some tentative indication on the positions of linkage on several monosaccharide residues. The fragments derived from these model oligosaccharides were used to propose a logical interpretation of the l.d.i.-F.t.-i.c.r. mass spectra of commercially available polysaccharide reported by others. Polysaccharides that have similar primary and tertiary structures gave similar mass spectra. This may be rationalized by the fact that fragmentation occurred at the sample layer or the selvedge. Consequently, the mass spectra reflected the primary and tertiary structures of the polysaccharides in the solid state. Finally, this l.d.i.-F.t.-i.c.r. technique was employed to sequence an unknown oligosaccharide obtained by anhydrous hydrogen fluoride degradation of the bacterial capsular polysaccharide. It was concluded that the unknown sample is composed of two tetrasaccharides with the same carbohydrate components, but with one structure substituted with serine and the other with threonine. Furthermore, indirect evidence suggests that the amino acids are linked to the carboxylic acid group of the hexuronic acid. However, two possible structures could be proposed for these oligosaccharides. The experimental results showed that l.d.i.-F.t.-i.c.r. mass spectrometry is well suited for the analysis of underivatized oligosaccharides, where the abundant ring fragments observed, can be used for a tentative indication of positions of linkage information. Furthermore, the high resolution data can be used to confirm the presence of unusual sugars and substituents. / Science, Faculty of / Chemistry, Department of / Graduate

No description available.

Thesis (Ph. D.)--West Virginia University, 2007. / Title from document title page. Document formatted into pages; contains x, 208, [233] p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 196-208).

Thesis--Wisconsin. / Vita. Includes bibliographical references (leaf 136).

Thesis (Ph.D.)--University of Nebraska-Lincoln, 2006. / Title from title screen (site viewed on Nov. 13, 2006). PDF text: 248 p. : col. ill. ; 6.20Mb. UMI publication number: AAT 3216336. Includes bibliographical references. Also available in microfilm and microfiche format.

No description available.

The structure of the capsular polysaccharides from Klebsiella serotype K60 and K26 have been determined using the techniques of methylation.periodate oxidation,partial hydrolysis, and β-elimination. ¹H- and ¹³C-n.m.r. spectroscopy was used to establish the nature of the anomeric linkages in both polysaccharides and also in the oligosaccharides obtained by the different degradative techniques used. Specific hydrolases obtained from bacteriophages were utilized to degrade two Klebsiella polysaccharides. Larger quantities of oligosaccharide repeating units can be generated in this manner. Two bacteriophages, Ø60 and Ø46,the first one with β-glucosidase activity and the other with β-galactosidase activity, were used to degrade the corresponding polysaccharides according to a new, simplified procedure. The purified gum exudate from Chorisia speciosa (palo borracho) was studied. The results from methylation analysis, β-elimination and partial hydrolysis made possible a tentative assignment for an "average structure" of the gum polysaccharide. / Science, Faculty of / Chemistry, Department of / Graduate

Thesis (doctoral)--Lund University, 1998. / Added t.p. with thesis statement inserted. Includes bibliographical references.

Thesis (doctoral)--Lund University, 1998. / Added t.p. with thesis statement inserted. Includes bibliographical references.