Lesbian, gay, bisexual, transgender and intersex people (LGBTI) are discriminated and subjected to violence in societies across the world, and ensuring their rights is on the international agenda. On a European level, nationalism, state-led persecution and rhetoric of hate have slowed down the process of ensuring human rights for LGBTI people particularly in Eastern Europe, where they are subject to violence, discrimination and hostility. One type of hostility targeting LGBTI people is hate speech. As literature has shown, hate speech can have harmful effects on its targets. Additionally, with the increased accessibility and use of the Internet and social media networks, hate speech has become more widespread and new challenges have appeared. Our research objective has addressed the gap identified by Brown (2018); to contribute to the research on hate speech and its harmful effects in general, comparing the effects of online and offline hate speech in particular. Additionally, by comparing the findings from Moldova and Ukraine, we have investigated if the context in which the hate speech is produced has any effect on the harmful effects experienced by targets. The research was conducted using a mixed method with a parallel convergent design, giving equal priority to qualitative and quantitative data. Data collection took place in Moldova (Chisinau) and in Ukraine (Kyiv) during Pride in the respective countries. Due to the nature of this research, results are not representative, and conclusions drawn can neither be applied to the entire LGBTI community in Moldova nor in Ukraine. Conclusions can, however, provide interesting insights for further research. Constitutive and consequential harms from online and offline hate speech are experienced by the LGBTI activists and community in both countries. In terms of constitutive harms, LGBTI community have suffered from harms like negative impact on self-esteem, silencing, psychological distress and restrictions on freedom of movement and association. Experienced consequential harms were negative stereotyping, physical violence and normalization of discrimination. Further, this research indicated that there is a difference in terms of harms caused by online and offline hate speech when it comes to the constitutive harms, as the harms from offline hate speech seemed to be experienced to a larger extent. Comparing results from Moldova and Ukraine, it can be concluded that the content of hate speech and harmful effects of hate speech are experienced differently depending on the context in which hate speech was produced. In general, hate speech in Ukraine seemed to be more violent and aggressive while in Moldova it was more related to the structural violence.