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Tripyrrine - Koordinationschemie an einem Porphyrinfragment Teil A /Brandt, Carsten D. Brandt, Carsten D. Brandt, Carsten D. January 1900 (has links)
Würzburg, Univ., Diss., 2003. / Dateien im PDF-Format. Erscheinungsjahr an der Haupttitelstelle: 2002. Computerdatei im Fernzugriff.
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Tripyrrine - Koordinationschemie an einem Porphyrinfragment Teil A /Brandt, Carsten D. Brandt, Carsten D. Brandt, Carsten D. January 1900 (has links)
Würzburg, Univ., Diss., 2003. / Dateien im PDF-Format. Erscheinungsjahr an der Haupttitelstelle: 2002. Computerdatei im Fernzugriff.
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Tripyrrine - Koordinationschemie an einem Porphyrinfragment Teil A /Brandt, Carsten D. January 1900 (has links)
Würzburg, Universiẗat, Diss., 2003. / Dateien im PDF-Format. - Erscheinungsjahr an der Haupttitelstelle: 2002.
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Etablierung und Anwendung von biophysikalischen und molekularbiologischen Methoden zur Untersuchung von Wirkmechanismen neuer Indol-, Carbazol- und Oligopyrrolcarboxamid-Derivate als potentielle antitumoraktive StoffeMarotto, Annalisa. Unknown Date (has links) (PDF)
Universiẗat, Diss., 2002--Mainz.
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Tripyrrine - Koordinationschemie an einem Porphyrinfragment ; Kristallstrukturanalysen metallorganischer und koordinationschemischer Verbindungen / Tripyrrins - Coordinationchemistry with a porphyrin fragment ; X-ray crystal structural analysis of metallorganic and coordination compoundsBrandt, Carsten D. January 2002 (has links) (PDF)
Gegenstand der vorliegenden Arbeit ist die Darstellung und Untersuchung von einfachen Triyrrinen. Dabei wurde ein besonderer Schwerpunkt auf die Entwicklung der Koordinationschemie dieses Liganden gelegt. Der zweite Teil der Arbeit beschäftigt sich mit der Durchführung von Röntgenstrukturanalysen metallorganischer und koordinationschemischer Verbindungen. Den Hintergrund für den ersten Teil bilden die jüngsten Versuche anderer Forschergruppen, mit den innerhalb der Porphyrinchemie kaum beachteten offenkettigen Tetrapyrrolen vom Bilen-Typ Phänomene der molekularen Erkennung, der supramolekularen Chemie und der Bioanorganik koordinationschemisch zu bearbeiten. Die Thematik ist zudem von Interesse, da anders als bei tetrapyrrolischen Liganden kaum etwas über das koordinationschemische Verhalten tripyrrolischer Spezies bekannt ist. Gerade das Tripyrrin erscheint hier als interessanter Modellligand, denn durch Wegnahme einer Pyrroleinheit wird eine neue, freie Koordinationsstelle geschaffen, deren Einfluß die Chemie der Tripyrrinate bestimmen sollte. In Kapitel 1 wird die Synthese der Tripyrrine aus pyrrolischen Vorstufen durch eine Kondensationsreaktion in Trifluoressigsäure beschrieben. Der Tripyrrin-Ligand erweist sich gegenüber Nukleophilen als höchst reaktiv, was wahrscheinlich der Grund dafür ist, daß dieser Ligand bislang nur in einer Arbeit beschrieben wurde. Eine Isolierung gelingt zwar nicht, wohl aber eine spektroskopische in situ-Charakterisierung mit Hilfe von NMR- und MS-Methoden. Die direkte Umsetzung der erhaltenen Rohprodukte mit überschüssigen Metall(II)acetaten (M = Fe, Mn, Co, Ni, Pd, Cu, Zn) führt in allen Fällen zu grün gefärbten Lösungen, aus denen sich für M = Co, Pd, Cu und Zn Tripyrrinkomplexe mit zweiwertigem, tetrakoordinierten Metallion und Trifluoracetat als viertem Donor isolieren lassen. Strukturell werden drei unterschiedliche Geometrien beobachtet. Das bevorzugt planar koordinierende Ion Pd(II) liefert Beispiele für den helikalen und den pseudoplanaren Strukturtyp, da aus sterischen Gründen die Ausbildung einer spannungsfreien planaren Molekültopologie unmöglich ist. Auch Cu(II) koordiniert als Trifluoracetat in der pseudoplanaren Variante, während Zn(II) in der nicht gespannten pseudotetraedrischen Form gebunden wird. Die in den Palladium-Komplexen vorhandenen Spannungen bewirken schnelle Ligandenaustauschreaktionen mit Halogeniden und Pseudohalogeniden. Bei den Strukturen der so zugänglichen TrpyPdX-Komplexe mit X = Cl, Br, I, N3, NCO, NCS, NO3, CN und StBu zeigt sich, daß mit zunehmender Größe des anionischen Donors die pseudoplanare Geometrie gegenüber der helikalen zunehmend begünstigt wird. Für Kupfer(II)-Komplexe wird beim Übergang vom Trifluoracetat zum Chlorid ein Wechsel von der gespannten pseudoplanaren zur wenig gespannten pseudotetraedrischen Koordination beobachtet. Die sterisch gespeicherte Spannungsenergie der Tripyrrine läßt tetrakoordinierte Pd(II)-Komplexe wie eine gespannte Feder erscheinen und unterstützt den Austritt des anionischen Liganden unter Bildung eines koordinativ und elektronisch ungesättigten 14 VE-Komplexes. Entscheidend für die Stabilisierung dieser Spezies ist die Verwendung des schwachkoordinierenden Tetrakis[3,5-bis(trifluormethyl)-phenyl]borats [B(Arf)4] als Anion. Der ungesättigte Komplex erweist sich als sehr reaktiv. So koordiniert er bereitwillig an eine Vielzahl von Donoren. Die Umsetzung des Trifluoracetato-Komplexes mit einem halben Äquivalent NaB(Arf)4 führt zu dinuklearen Komplexen, in denen zwei kationische Tripyrrinatopalladium-Fragmente durch ein Trifluoracetat verbunden sind. Mit Trialkylphosphanen bilden sich stabile Komplexe. Eine Besonderheit stellt dabei die Reaktion mit Trimethylphosphan dar. Bei Verwendung überschüssiger Mengen PMe3 beobachtet man die Bildung pentakoordinierter Komplexe. Im Gegensatz dazu führen die Umsetzungen mit Triethyl- und Tri-iso-propylphosphan ausschließlich zur Bildung von Monophosphankomplexen. Die ungewöhnliche Reaktivität des Tripyrrinatopalladium-Kations zeigt sich insbesondere bei der Umsetzung mit Diazoalkanen. So konnten erstmals Carbenpalladium-Komplexe mit nicht-heteroatomstabilisierten Carbenliganden synthetisiert werden. Kapitel 5 beschreibt einen präparativen Einstieg in die Chemie kationischer Kobalt- und Zinkkomplexe von Tripyrrinen. Die Reaktivität und Stabilität des Tripyrrinatokobalt-Kations, die an die Verhältnisse des TrpyPd-Kations erinnern, erlauben dabei die Isolierung von kationischen Phosphan- und Isonitril-Komplexen. Das entsprechende kationische Zink-Chelat konnte isoliert und NMR-spektroskopisch charakterisiert werden. / Part I of the presented work describes the preparation and investigation of simple tripyrrin ligands, with a special emphasis on the development of a functional coordination chemistry of this ligand. Part II deals with x-ray crystallographic work performed on organometallic and classical coordination compounds. The recent attempts from other research groups to use open-chain oligopyrroles related to the bile pigments as ligands for novel developments in the fields of molecular recognition, supramolecular and bioinorganic chemistry provided the motivation for the first part of this work. Since almost nothing was known about the coordination behaviour of open-chain oligopyrroles, especially of those with three pyrrolic subunits, a first principal investigation towards the properties of metallotripyrrins appeared as a suitable entry into this field. With respect to the porphyrins, the formal withdrawal of one of the pyrrolic units should create a new, free coordination site at a bound metal ion. This free site is expected to determine the chemistry of metallotripyrrins largely. The synthesis of tripyrrins from pyrrolic precursors by a condensation reaction in trifluoroacetic acid is described in chapter 1. The tripyrrin ligand system is found to be unusually reactive towards even weak nucleophiles. Isolation of the ligand was not successful. However, tripyrrins could unambigously be characterised by a spectroscopic in situ-characterisation using NMR- and MS-techniques. When treating the raw tripyrrin ligands with excessive metal acetates of Fe, Mn, Co, Ni, Pd, Cu or Zn, green solutions are formed in all cases, from which the tripyrrinato complexes of M = Co, Pd, Cu and Zn with divalent tetracoordinated metal ions and trifluoroacetate as the fourth donor can be isolated. From a structural point of view, three different molecular geometries were observed. The Pd(II) ion with its pronounced tendency to establish a square-planar coordination mode yields examples for the strained helical and the pseudoplanar structures. A non strained square planar complex geometry is prevented for sterical arguments due to the presence of the two methyl termini in all new tripyrrin complexes. The trifluoroacetate derivatives of Cu(II) also are found to form pseudoplanar coordination geometries, while Zn(II) always prefers a non-strained pseudotetrahedral variant. The strain stored in the Pd(II) complexes is responsible for the fast ligand exchange reactions of the trifluoroacetate derivatives against halide and pseudohalide anions. In the group of TrpyPdX compounds with X = Cl, Br, I, N3, NCO, NCS, NO3, CN and StBu the pseudoplanar geometry becomes more important then the helical binding mode with an increasing radius of the donor atom of the fourth ligand. The strain energy stored in the Pd(II) tripyrrins was found to support the dissociation of the fourth ligand, yielding the coordinatively and electronically unsaturated 14 VE complex. In order to stabilise this highly reactive species the use of a weakly coordinating anion is of vital importance, and the well-known tetrakis[3,5-bis(trifluormethyl)phenyl]borat [B(Arf)4] was found to be sufficiently stable to fulfil this task. As expected, the coordinatively and electronically unsaturated Pd(II) complex proofs to be very reactive and binds tenaciously to a variety of different donor ligands. As a special case, the reaction of the cation with one equivalent of the tripyrrinatopalladium trifluoroacetate complex yields a dinuclear species, in which two cationic TrpyPd fragments are connected via one trifluoroacetato ligand. Trialkylphosphanes, however, are able to stabilise cationic tetracoordinated species. While usually only four coordinate PdN3P compounds are obtained, the action of trimethylphosphane is different. Pentacoordinate Pd(II) complexes are formed in the presence of excess PMe3. These species, which could be structurally characterised in this thesis, were found to be in a slow equilibrium with the respective mono PMe3 adducts. The unusual reactivity of the tripyrrinatopalladium cation is particularly well visible in its reactions towards diazoalkanes. When treated with the sterically demanding diaryldiazomethane, the first carbene palladium(II) complexes of non heteroatom stabilised carbene ligands were prepared and found to exist as stable compounds at room temperature. Chapter 5 finally describes a preparative entry into the chemistry of cationic cobalt(II)- and zinc(II) tripyrrins. The reactivity and stability of the tripyrrinatocobalt(II) cation is reminescent of the respective species in the palladium(II) series and allows the preparation and isolation of cationic phosphane- and isonitrile complexes. For zinc(II) as the metal a related tricoordinate cation could be isolated and investigated by nmr spectroscopy.
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Quantum-chemical Study Of Geometrical And Electronic Structures Of Aromatic Five-membered Heterocyclic Oligomers In The Ground And Lowest Singlet Excited StatesOksuz, Nevin 01 September 2004 (has links) (PDF)
The nature of the ground state and the first (lowest) singlet excited state geometrical conformations and electronic transitions in the aromatic five-membered heterocyclic oligomers &ndash / oligothiophenes (nT), oligofurans (nF), and oligopyrroles (nP)- containing up to six monomer units (total of 18 molecules) were explored using several computational methodologies. Geometry optimizations were carried out at Austin Model 1 (AM1), Restricted Hartree-Fock (RHF/6-31G*), and Density Functional Theory (DFT, B3LYP/6-31G*) levels for the ground-state conformations of these structurally well-defined heterocyclic oligomers. The Configuration Interaction Singles (CIS) method with the 6-31G* basis set was chosen in computation of the optimal geometry of the lowest singlet excited state. Lowest singlet excitation S1ß / S0 energies were calculated using the Zerner&rsquo / s Intermediate Neglect of Differential Overlap for Spectroscopy (ZINDO/S), CIS (CIS/6-31G*), and Time-Dependent DFT (TDDFT/6-31G* and TDDFT/6-31+G*) methods. In computation of the emission S1à / S0 energies, we have employed all methods above except ZINDO/S.
In investigation of geometries of the ground and lowest singlet excited state, we compared the bond length alternation (BLA) parameters, Dri in the conjugated backbone of the oligomers. Saturation of the geometrical parameters at the center of oligomers was observed after a certain chain length.
Among all methodologies used in computation of excitation (S1ß / S0) and emission (S1à / S0) energies, TDDFT results showed the best agreement with experimental data. Fits of computed and experimental excitation energies to an exponential function using the least squares method enabled us to predict Effective Conjugation Length (ECL) values. We obtained the ECLs of 17 (17), 16 (15), and 14 (13) monomer units for polythiophene (PTh), polyfuran (PFu), and polypyrrole (PPr), which have very good agreement with the results obtained from the fits of experimental data (the values in parentheses).
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New DNA-Targeting Small Molecules as Potential Anticancer Agents and for in vivo Specificity toward Enhanced Silk ProductionAli, Asfa January 2014 (has links) (PDF)
The thesis entitled “New DNA-Targeting Small Molecules as Potential Anticancer Agents and for in vivo Specificity toward Enhanced Silk Production” encompasses design, computational calculations, and syntheses of diverse small molecular scaffolds to explicitly target duplex and higher order DNA morphologies (G-quadruplex DNA). Some of these molecules have a potential as anticancer agents. Besides, an attempt has been made elucidate the importance of novel oligopyrrole carboxamides in the enhancement of silk yield, hence proving to a boon in the field of sericulture. The work has been divided into six chapters.
Chapter 1. DNA Binding Small Molecules as Anticancer Agents
Figure 1. DNA targeting by small molecules.
Cancer has always been a dreadful disease and continues to attract extensive research investigations. Various targets have been identified to restrain cancer. Among these DNA happens to be the most explored one. A wide variety of small molecules, often referred to as “ligands”, has been synthesized to target numerous structural features of DNA (Figure 1). The sole purpose of such molecular design has been to interfere with the transcriptional machinery in order to drive the cancer cell toward apoptosis. The mode of action of the DNA targeting ligands focuses either on the sequence-specificity by groove binding and strand cleavage, or by identifying the morphologically distinct higher order structures like that of the G-quadruplex DNA.
Chapter 2. Ligand 5, 10, 15, 20-tetra(N-methyl-4-pyridyl)porphine (TMPyP4) Prefers the Parallel Propeller-type Human G-Quadruplex DNA over its other Polymorphs
The binding of ligand 5, 10, 15, 20-tetra(N-methyl-4-pyridyl)porphine (TMPyP4) with telomeric and genomic G-quadruplex DNA has been extensively studied. However, a comparative study of interactions of TMPyP4 with different conformations of human telomeric G-quadruplex DNA, namely parallel propeller-type (PP), antiparallel basket-type (AB), and mixed hybrid-type (MH) G-quadruplex DNA has not been done. We considered all the possible binding sites in each of the G-quadruplex DNA structures and docked TMPyP4 to each one of them. The resultant most potent sites for binding were analyzed from the mean binding free energy of the complexes. Molecular dynamics simulations were then carried out and analysis of the binding free energy of the TMPyP4-G-quadruplex complex showed that the binding of TMPyP4 with parallel propeller-type G-quadruplex DNA is preferred over the other two G-quadruplex DNA conformations. The results obtained from the change in solvent excluded surface area (SESA) and solvent accessible surface area (SASA) also support the more pronounced binding of the ligand with the parallel propeller-type G-quadruplex DNA (Figure 2).
Figure 2. Ligand TMPyP4 prefers parallel propeller-type G-quadruplex DNA morphology.
Chapter 3. A Theoretical Analysis on the Selective Stabilization of Intermolecular G-quadruplex RNA with a bis-Benzimidazole Ligand EtBzEt over TMPyP4 in K+ Environment
Ever since the discovery of G-quadruplex RNA, a constant urge exists to target these higher order RNA conformations. These structures play a significant role in the transcriptional and translational mechanism. Herein we have determined the mode and extent of association of certain G-quadruplex DNA binding bisbenzimidazole ligand (EtBzEt) in comparison to a known porphyrin ligand (TMPyP4). We have performed docking studies of the known G-quadruplex DNA binding ligands with the parallel propeller G-quadruplex RNA (PPR) to determine the most potent binding conformation which showed EtBzEt to be a better RNA binder than others. Furthermore, a molecular dynamics (MD) simulation (6 ns) was performed for the most stable docked complex in explicit solvent environment. The role of K+ ions, Hoogsteen hydrogen bond formation and backbone dihedral angle between the tetrads were carefully analyzed during the entire simulation run to determine the stability of each ligand associated PPR complex. All the analyses conclusively showed that while TMPyP4 merely stabilized the PPR, the ligand EtBzEt stabilized PPR very efficiently (Figure 3).
Figure 3. Stabilzation and destabilization by EtBzEt and TMPyP4, repectively. Red and green ovals represent EtBzEt and TMPyP4, repectively.
Chapter 4A. Design and Synthesis of New DNA Binding Fe(III) and Co(II) Salen Complexes with Pendant Oligopyrrole Carboxamides
Extensive research on these oligopyrrole carboxamides has shown their specificity toward AT-rich sequences with high binding affinity. Here we have designed and synthesized Fe (III)-and Co (II)-based salen complexes attached with minor groove targeting oligopyrrole carboxamide side-chains (Figure 4). While the ligands showed excellent activity toward DNA damage, they also exhibited high affinity toward the minor grooves of the ds-DNA. This was also reflected in the high efficiency of the ligands toward cancer cell cytotoxicity.
Further studies revealed that the ligands resulted in prominent nuclear condensation and fragmentation thereby driving the cells toward apoptosis. The presence of metal coordinated salen moiety conjugated with positively charged pendants ending with minor groove binding oligopyrrole carboxamides might have resulted in the increased activity of the ligands toward DNA targeting and cancer cell death.
Figure 4. Chemical structures of the ligands used in this study.
Chapter 4B. Design and synthesis of novel oligopyrrole based salen metal complexes and their efficiency toward stabilization of G-quadruplex DNA
DNA targeting has been the key strategy toward the restriction of cancer cell proliferation. In a similar effort, we have designed and synthesized novel salen based Ni(II) and Pd(II) metal complexes with positively charged flanking side-chains comprising attached N-methylpyrrole carboxamides of varying lengths (Figure 5). The ligands showed efficient stabilization of the G-quadruplex DNA morphologies, with specificity over the duplex DNA. Sufficient inhibition of the telomerase activity was observed by the TRAP-LIG assay which was ascertained by the prominent restriction of cancer cell proliferation in the long-term cell viability assay. The ligands exhibited condensation and fragmentation of the nucleus when observed under confocal microscopy which is indicative of the cells undergoing apoptosis. Further annexin V-FITC and PI dual staining showed apoptosis to be the mechanistic pathway underlying the cancer cell cytotoxicity by the ligands. Modeling studies clearly showed the stacking of the salen moiety over the G-tetrads with the association of the pendant oligopyrrole carboxamide units to the grooves.
Figure 5. Chemical structures of the ligands used in this study.
Chapter 5A. Role of Metal Ions in Novel Fluorescein based Salen and Salphen Complexes toward Efficient DNA Damage and their Effect on Cancer Cells
Metal ions play an important role toward DNA damage and numerous ligands have been synthesized for their use in anticancer therapy. Herein, we have designed and synthesized Fe(III) and Co(II) based salen/salphens by bridging two fluorescein moieties with varying spacers (Figure 6). Although the ligands exhibit dual binding mode, the more flexible salen ligands prefer to associate to the minor groove of the DNA while the relatively rigid salphen ligands show greater intercalation. The biophysical experiments reveal better binding affinity of the salphens toward duplex DNA as compared to the salen ligands. The metal coordination resulted in efficient DNA cleavage of plasmid at low ligand concentrations. The ligands also showed cancer cell cytotoxicity, cellular internalization with apoptosis as the proposed mechanism for cell death.
Figure 6. Chemical structures of the salen and salphen ligands used in this study.
Chapter 5B. Fluorescein based Salen and salphen Complexes as stabilizers of the Human G-quadruplex DNA and Promising Telomerase Inhibitors
Metal based salen complexes have been considered as an important scaffold toward targeting of DNA structures. In the present work we have designed and synthesized nickel(II)-and palladium(II)-salen and salphen ligands by using fluorescein as the backbone to provide an extended aromatic surface (Figure 7). The ligands exhibit sufficient affinity toward the human telomeric G-quadruplex (G4) DNA in preference to the duplex DNA and also exhibit promising inhibition of telomerase activity. This is ascertained by their potency in the long-term cell viability assay which shows significant cancer cell cytotoxicity in presence of the ligands. Confocal microscopy showed cellular internalization followed by nuclear localization. Considerable population at the sub-G1 phase of the cell cycle showed cell death via apoptotic pathway.
Figure 7. Chemical structures of the ligands used in this study.
Chapter 6. Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole carboxamides Enhances Silk Production
Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of
B. mori is enriched with 5′-TTAGG-3′ sequences. These sequences were also found to be present in several genes in the euchromatic regions. We examined three synthetic oligopyrrole carboxamides that target 5′-TTAGG-3′ sequences in controlling the gene expression in B. mori (Figure 8). The ligands did not show any defect or feeding difference in the larval stage, crucial for silk production. The compounds caused silencing of various isoforms of the broad-complex transcription factor and cuticle proteins which resulted in late pupal developmental defects. This study shows for the first time use of oligopyrrole carboxamide drugs in controlling gene expression in B. mori and their long term use in enhancing silk production.
Figure 8. Chemical structures of the ligands used in this study (top) and increased cocoon size on ligand treatment.
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