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Characterization and applications of soft tissue mimicking materials are presented. A skin mimicking material and a skin mimicking gel is characterized for industrial, scientific, and medical (ISM) band (2.40 GHz-2.48 GHz). Also a wide band (0.3 GHz – 2.5 GHz) muscle mimicking material is developed. A dual band implantable antenna operating at medical implant communication service (MICS) band (402 MHz – 405 MHz) and ISM band is tested in vitro with skin mimicking material for ISM band. MICS band measurements of the implantable antenna tested in vitro by placing the antenna on the interface of muscle and skin mimicking gel. An on-body antenna operating at ISM Band is designed for wireless cardiac monitoring applications. The mutual coupling between the antennas is minimized by placing antennas with 90 degree phase difference. The recipes for tissue mimicking materials and results such as, comparison of electrical properties, return loss, and mutual coupling measurements is given.