Submitted by Verena Bastos (verena@uefs.br) on 2015-07-27T22:22:25Z No. of bitstreams: 1 DISSERTA??O_Robson Dias.pdf: 1622045 bytes, checksum: 199cc0a1bb678a7d768d85efa421b8b3 (MD5) / Made available in DSpace on 2015-07-27T22:22:25Z (GMT). No. of bitstreams: 1 DISSERTA??O_Robson Dias.pdf: 1622045 bytes, checksum: 199cc0a1bb678a7d768d85efa421b8b3 (MD5) Previous issue date: 2014-09-29 / Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP / The Lamiaceae family has great economic importance as a source of essential oils, in addition to being well studied from a chemical standpoint. Pharmacological studies reports are also extensive in the scientific literature because the specimens of this family are found in various parts of the world. This study aims to evaluate the chemical composition and antinociceptive activity in mice of the essential oils obtained from the leaves of Oocephalus nubicola and Leptohyptis macrostachys. The plant material was collected in the semiarid region of Bahia. The essential oils were obtained by hydrodistillation (3 h), on a Clevenger-type apparatus. The oils were analyzed by capillary gas chromatography using flame ionization detection and mass spectrometry. Two general pharmacological tests were used to assess possible effects of the extracts on the central nervous system of animals: the screening behavioral pharmacology and the rota-rod test. Antinociceptive effects were assessed using acetic acid-induced writhing test and formalin test. Both oils were presented as a mixture of monoterpenes and sesquiterpenes, and Oocephalus nubicola showed predominance of sesquiterpene components, while in the Leptohyptis macrostachys monoterpenes were major. The pharmacological evaluations demonstrated that the samples did not affect motor coordination of animals, besides their antinociceptive properties in the three experimental doses, with evidence of central effects. / A fam?lia Lamiaceae tem grande import?ncia econ?mica por ser fonte de ?leos essenciais arom?ticos, al?m de ser bem estudada do ponto de vista qu?mico. Relatos de estudos farmacol?gicos tamb?m s?o extensos na literatura cient?fica, pois as esp?cimes desta fam?lia s?o encontradas em v?rias partes do mundo. O presente trabalho tem como objetivo avaliar a composi??o qu?mica e a atividade antinociceptiva em camundongos dos ?leos essenciais obtidos das folhas de Oocephalus nubicola e Leptohyptis macrostachys. O material vegetal foi coletado na regi?o do semi?rido do Estado da Bahia. Os ?leos essenciais foram obtidos por hidrodestila??o (3 h), em um aparelho do tipo Clevenger. Os ?leos foram analisados por cromatografia capilar gasosa, usando detec??o de ioniza??o de chama e espectrometria de massas. Dois testes farmacol?gicos gerais foram aplicados para avalia??o de poss?veis efeitos das amostras no Sistema Nervoso Central dos animais: triagem farmacol?gica comportamental e teste da barra girat?ria. Para avalia??o da atividade antinociceptiva foram empregadas duas metodologias: o teste das contor??es abdominais induzidas pelo ?cido ac?tico e o teste da formalina. Ambos os ?leos apresentaram-se como uma mistura de monoterpenos e sesquiterpenos, sendo que Oocephalus nubicola apresentou predomin?ncia dos constituintes sesquiterp?nicos, enquanto que em Leptohyptis macrostachys os monoterpenos foram majorit?rios. As avalia??es farmacol?gicas demonstraram que as amostras n?o afetam a coordena??o motora dos animais, al?m de possu?rem propriedades antinociceptivas nas tr?s doses experimentais, com ind?cios de mecanismo de a??o central.