M.Sc. / Ophiopogon planiscapus var. nigrescens belongs to the family Convallariaceae. This plant exhibits unique black pigmented leaves and is, therefore. used in growing arrangements. The natural rate of multiplication. however. is slow and a reversion to green leaves in some plants necessitates the replacement of these plants, which is a time consuming activity. In vitro techniques allow the establishment of black clones and also speed up the multiplication process. A cultivation medium for optimal growth was formulated. The influence of other parameters on growth. multiplication and rooting was investigated and final hardening was done to yield plants suitable for the greenhouse.