Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-20T21:49:24Z No. of bitstreams: 1 AlannaDeSousaRodrigues_DISSERT.pdf: 996440 bytes, checksum: d75b3da15e011ba5988e8e2aad479a21 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-23T18:33:46Z (GMT) No. of bitstreams: 1 AlannaDeSousaRodrigues_DISSERT.pdf: 996440 bytes, checksum: d75b3da15e011ba5988e8e2aad479a21 (MD5) / Made available in DSpace on 2017-03-23T18:33:46Z (GMT). No. of bitstreams: 1 AlannaDeSousaRodrigues_DISSERT.pdf: 996440 bytes, checksum: d75b3da15e011ba5988e8e2aad479a21 (MD5) Previous issue date: 2003-08-29 / Embora o aumento na concentra??o de Esp?cies Reativas de Oxig?nio (Eros), devido ao diabetes mellitus, pode alterar o metabolismo normal do osso, acarretando assim a osteopenia. Uma suplementa??o com c?lcio, f?sforo e vitamina E pode ter a??o ben?fica e prevenir as complica??es associadas ao estado diab?tico. Foram utilizados ratos Wistar machos (n=125), peso corporal 250-350g, sendo divididos em tr?s grupos: grupo 1- controles (n=45) grupo 2- diab?ticos experimentais (n=45), e grupo 3 - diab?ticos suplementados (n=35) em c?lcio, f?sforo e vitamina E. O diabetes experimental foi induzido por estreptozotocina (STZ) (45 mg/Kg) e sacrificados em per?odos de 1 e 5 dias ap?s a indu??o e 5, 30, 60 e 90 dias ap?s a instala??o do diabetes mellitus. Animais com glicemia ? 250 mg/dL foram considerados diab?ticos. Realizaram-se an?lises de Subst?ncias Reativas ao ?cido Tiobarbit?rico (SRAT) e Glutationa (GSH) em sangue e c?rebro, medidas histomorfom?tricas dos f?mures esquerdos, al?m de dosagens bioqu?micas de fosfatase alcalina, fosfatase ?cida, fosfato, magn?sio, c?lcio ioniz?vel, c?lcio total, creatinina e albumina em amostras de soro e glicose, fosfato, magn?sio, creatinina e clearence de creatinina em amostras de urina dos animais. As SRAT no plasma e c?rebro, apresentaram-se significativamente elevadas no grupo 2 em rela??o ao grupo 1, e diminu?da para o grupo 3, no per?odo de 90 dias. O conte?do de GSH em c?rebro, no per?odo de 90 dias, diminuiu no grupo 2 e aumentou no grupo 3, enquanto em sangue n?o foram observadas altera??es. Os resultados histomorfom?tricos mostraram uma perda em m?dia de 50% de osso trabecular avaliadas pela dist?ncia entre as trab?culas, espessura das trab?culas e volume ?sseo trabecular, nos animais diab?ticos experimentais em rela??o aos controles e, com a suplementa??o, essa perda diminuiu significativamente alcan?ando valores pr?ximos aos encontrados no grupo controle. A atividade da fosfatase alcalina apresentou valores superiores para os grupos 2 e 3 em rela??o ao grupo 1 e a fosfatase ?cida aumentou significativamente nos grupos 2 e 3, em rela??o aos controles, no per?odo de 90 dias. As concentra??es s?ricas de fosfato, magn?sio, c?lcio ioniz?vel, c?lcio total e creatinina, n?o apresentaram altera??es uma vez que os resultados est?o dentro dos valores de intervalos de refer?ncia. A albumina s?rica mostrou-se diminu?das para o grupo 2 e 3. A an?lise da urina mostrou glicos?ria significativa para os grupos 2 e 3 em rela??o ao grupo 1. Em rela??o a fosfat?ria observou-se um aumento significativo principalmente para o grupo 3 e em rela??o ao clearence de creatinina n?o foram observadas diferen?as significativamente para o grupo 2 e 3. Os resultados observados sugerem que a suplementa??o de c?lcio, f?sforo e vitamina E diminuiu as altera??es oxidativas causadas pelo diabetes, al?m de ter exercido a??o ben?fica sobre o metabolismo ?sseo, diminuindo a perda ?ssea que ocorre durante esta s?ndrome. / The increase of EROs concentration, through diabetes mellitus, can alter the normal bone metabolism, causing osteopenia. A calcium, fosforum and vitamin E supplementation, can be a very good action in bone. In thi study were used male Wistar rats (n=125), weight ranging from 250 ? 350g, and were divided in three groups: group 1 control (n=45) group 2 experimental diabetic (n=45) and group 3 supplementadies diabetic (n=35) in calcium, fosforum and vitamin E. The animals were inuced with streptozotocin (45mg/Kg ) and killed in periods of 1 to 5 days after the indution e 5, 30, 60 and 90 days after installation of experimental diabetes mellitus. Animals with glycemia ? 250 mg/ dL was considered diabetics.In each period was realized determinations of Thiobarbituric Acid Reactive Substances and levels of reduced glutathione in blood and brain, histomorfometric measurement of left fermurs, and dosages of alkaline phosphatase, acid phosphatase, ionizable calcium, total calcium, phosphorus, magnesium, glycemia, creatinin and albumin in serum and glucose, phosphorus, magnesium, creatinine and clearence of creatinin in urine. Thiobarbituric Acid Reactive Substances at brain and plasma, choose higher at group 2 in relation group 1, and reduced in group 3, in period of 90 days. The levels of reduced glutathione in brain and blood, at 90 days, was decreased in group 2 and increased at group 3. The histomorphometric results show a less in middle of 50% in trabecular bone volume analysed per distance between the trabeculaes thickness of trabeculae and bone quantity in experimental diabetic animals in comparison to the control group and for controls group and, supplemented animals this lose reduced very much arriving sizes near found in control group. The activity of alkaline phosphatase showed superior values in relation of groups 2 and 3, about comparing group 1 and acid phosphatase increased higher in groups 2 and 3, about all controls, in a 90 days. The serum concentration of phosphorus, magnesium, ionazle calcium, total calcium and creatinine, didn?t show alterations, and the results are well if compare the reference result. The albumin serum showed less to group 2 and 3. The urine analyse showed significative glycos?ria to groups 2 and 3 if compared with group 1. In relation phosphatury, observed a significative increase principally to group 3 and creatinine clearance relation wasn?t observed differences to group 2 and 3. The results observed suggest that calcium, phosphorus and vitamin E supplementation, decreased the oxidative alterations which the diabetics were the cause, and helped bone metabolism, decreasing the lose bone that ocurr in this syndrome

Made available in DSpace on 2014-12-17T14:13:36Z (GMT). No. of bitstreams: 1 ValeriaMGDML_TESE.pdf: 5877760 bytes, checksum: 33cd720eef775e51bdd077da12942ba1 (MD5) Previous issue date: 2010-11-19 / Funda??o de Amparo a Pesquisa do Estado de S?o Paulo / The relationship between lipid peroxidation, antioxidant defense and diabetic osteopenia remains unclear. This study evaluated the relationship between lipid peroxidation index, antioxidant defense parameter and bone metabolism in a premenopausal diabetic model by measurements such as thiobarbituric acid-reactive substances concentration (TBARS) and reduced glutathione (GSH) content in brain homogenates, histomorphometric analysis, biomechanical testing and bone mineral density (BMD). Female Wistar rats with regular estrous cycle were divided into two groups: Group 1: control rats (n = 15) and Group 2: diabetic rats (n = 15). Diabetes mellitus was induced by alloxan and confirmed by glycemia &#61619;250 mg/dL. The experimental period understood 1 and 5 after days induction and 45, 75 and 120 days after the installation of diabetes mellitus.The lipid peroxidation index, measured by TBARS concentration, showed a significant increase (p<0.05) in diabetic animals in comparison to animals control. However, the antioxidant parameter, measured by GSH content, was significantly decrease (p<0.05) in diabetic animals. Histomorphometric analysis showed a significant increase (p<0.05) in femoral trabecular separation together with a significant decrease (p<0.05) in trabecular thickness and reduced trabecular bone volume in diabetic rats. Moreover, biomechanical testing and BMD values were significant decrease (p<0.05) in diabetic group. Thus, our results demonstrated that increased lipid peroxidation and altered antioxidant defense could be related to the development of oxidative stress and diabetic osteopenia in premenopausal rats / A rela??o entre peroxida??o lip?dica, defesa antioxidante e osteopenia diab?tica permanece obscura. Este estudo avaliou a associa??o entre ?ndice de peroxida??o lip?dica, par?metro de defesa antioxidante e metabolismo ?sseo em um modelo diab?tico pr?-menopausado atrav?s de medidas como a concentra??o de subst?ncias reativas ao ?cido tiobarbit?rico (SRAT) e conte?do de glutationa reduzida (GSH) no homogenato cerebral, an?lises histomorfom?tricas, teste biomec?nico e densidade mineral ?ssea (DMO). Ratas Wistar com ciclo estral regular foram distribu?das em dois grupos: Grupo 1 - ratas controle (n = 15) e Grupo 2 - ratas diab?ticas (n = 15). O diabetes mellitus foi induzido pela aloxana e confirmado pela glicemia &#61619;250 mg/dL. O per?odo experimental compreendeu 1 e 5 dias ap?s a indu??o e 45, 75 e 120 dias ap?s a instala??o do diabetes mellitus. O ?ndice de peroxida??o lip?dica, medido pela concentra??o de SRAT, demonstrou um aumento significativo (p<0.05) nos animais diab?ticos, em rela??o aos animais controle. Entretanto, o par?metro de defesa antioxidante, mensurado pelo conte?do de GSH, foi reduzido significativamente (p<0.05) nos animais diab?ticos. As an?lises histomorfom?tricas mostraram um aumento significativo (p<0.05) da separa??o trabecular do f?mur, associado ? diminui??o significativa da espessura trabecular (p<0.05) e volume ?sseo trabecular reduzido nas ratas diab?ticas. Al?m disso, o teste biomec?nico, medido pela for?a m?xima de compress?o, e valores de DMO foram reduzidos significativamente (p<0.05) no grupo diab?tico. Dessa maneira, nossos resultados demonstraram que a peroxida??o lip?dica aumentada e defesa antioxidante modificada podem estar relacionadas ao desenvolvimento do estresse oxidativo e osteopenia diab?tica em ratas pr?-menopausadas