This study is based on the Owen-Wendt-Rabel and Kaelble method¡Aand we using several substrates which's surface energy is already known to determind the surface eneygy of liquid. We measure the contact angle of the liquid on the substract to calculate the dispers part and the polar part of the surface enerfy. And then we compare the system with different sets of substrates and choose one proper set to be the standard substrates.Finally we test the system by several common liquid which¡¦s surface energy is already known,and measure the surface energy of liquid crystal. In this study,we found that the surface energy of the liquid crystal on an alignment layer is anisotropy,and discuss requirements of the standard substrate.