Mid-rotation fertilization and vegetation control are some of the most common silvicultural treatments in loblolly pine (Pinus taeda L.) plantations in the southeastern United States. Competing vegetation is commonly thought to sequester fertilizer nitrogen (N) and reduce the potential growth response to a mid-rotation fertilization treatment. This experiment aims to identify what proportion of applied N fertilizer is retained in the crop tree pine foliage, and the degree to which understory vegetation is competing for this resource. Our mid-rotation loblolly pine plantation received an application of 15N fertilization (urea 365 kg/ha, at 46% N by weight, i.e. 168 kg/ha of N) and a portion of plots received an understory vegetation control (basal spray application of triclopyr; 13.6% active ingredient) treatment either before fertilization or not at all. One-year post-fertilization, 15N contents within pine foliage, leaf fall/leaf litter, forest floor, and soil were measured, as was competing vegetation presence. There was significant variation in applied nitrogen acquisition among the different ecosystem components measured, with 0-15 cm soils retaining a majority at 32-37% added 15N. Differences in fertilizer N acquisition in pine foliage between plots with and without understory vegetation control was marginally significant (p = 0.06) with pine foliage in plots without understory vegetation capturing greater 15N (4.3% greater). Red maple (Acer rubrum) and oak species (Quercus spp.) were the most common competitors but neither had a uniquely pronounced effect on pine nitrogen sequestration. My data indicate that increasing competition reduces fertilizer N foliar concentrations in crop pine trees but at a modest rate and equally across species groups. An unrefined threshold determining when fertilizer N capture in crop pine trees was affected was found at 3.1 m2/ha of competing vegetation basal area. This site will continue to be monitored over time to assess fertilizer N retention in loblolly pine each year after fertilization and evaluate the fertilizer N capture within competing vegetation. / Master of Science / Some of the most prevalent management practices for mid-rotation (age 15, i.e., roughly halfway through a crop cycle) loblolly pine (Pinus taeda L.) plantations in the southeastern United States are fertilization and vegetation control. Nitrogen (N) is consistently one of the most limiting factors to productivity. The addition of N via fertilization is therefore a common forestry practice. However, when a stand is fertilized, the added resource is partitioned and cycled throughout the ecosystem. It is presumed that the amount of fertilizer N obtained by crop trees in a plantation is dependent on the level of competing vegetation (i.e., weed-trees and shrubs) present on site. Controlling competing vegetation prior to fertilization may therefore be warranted under certain conditions. To date, the amount of competing vegetation where it begins to impact fertilizer uptake by the crop tree is unknown. This study aims to elucidate this competing vegetation threshold to better inform mid-rotation management of loblolly pine plantations. This study examined applied fertilizer N capture in ecosystem components with varying levels of understory vegetation, and found more fertilizer N in pine foliage when understory vegetation was completely removed prior to fertilization. No single understory hardwood weed species had a uniquely strong influence on crop tree productivity uptake. Plots that ranked in the upper third in competing vegetation presence did have significantly less foliar fertilizer N in the pine crop trees. Additional replication of this study would be necessary to determine a universal threshold of competing vegetation which would trigger the removal of competing vegetation prior to fertilization.

Remote sensing offers many advantages to previous forest measurements, such as limiting costs and time in the field. Light detection and ranging (lidar) has been shown to enable accurate estimates of forest height. Lidar does produce precise measurements for ground elevation and forest height, where and when it is available. However, it is expensive to collect and does not have wall-to-wall coverage in the United States. In this study, we estimated height using the National Agricultural Imagery Program (NAIP) photogrammetric point clouds to create a predicted height map for managed loblolly pine stands in the southeastern United States. Recent studies have investigated the ability of digital aerial photogrammetry (DAP), and more specifically NAIP, as an alternative to lidar as a means of estimating forest height due to its lower costs, frequency of acquisition, and wall-to-wall coverage across the United States. Field-collected canopy height for 534 plots in Virginia and North Carolina were regressed against the 90th percentile derived from NAIP point clouds. The model for predicted pine height using the 90th percentile of height (P90) is predicted pine height = 1.09(P90) – 0.43. The adjusted R^2 is 0.93, and the RMSE is 1.44 m. This model is being used to produce a 5 m x 5 m canopy height model for all pine stands across Virginia, North Carolina, and Tennessee. NAIP-derived point clouds are thus a viable means of predicting canopy height in southern pines. / M.S. / Collecting accurate measurements of pine plantations is essential to managing them to maximize various ecosystem goods and services. However, it can be difficult and time-consuming to collect these measurements by hand. This study demonstrates that point clouds derived from digital stereo aerial photograms enable calculating forest height to an accuracy sufficient for pine plantation management. We developed a simple linear regression model to predict forest canopy height using the 90th percentile of the photo-derived heights above the ground in a given area. With this model, we created a map of pine plantation canopy heights (consisting of 5 m x 5 m grid cells, each containing a canopy height estimate) for pine forests in Virginia, North Carolina, and Tennessee. Digital aerial photography from the National Agricultural Imagery Program (NAIP) is repeated every three years for a given state, allowing growth to be mapped over time. Photography collected by NAIP and similar programs also has uniform acquisition parameters in a given year applicable over large regions. State- and national photography programs like NAIP are also less expensive than other data sets, like airborne laser scanning data, that enable estimation of tree height.

Studies were conducted to determine the incidence and severity of Heterobasidion annosum (Fr.) Bref. root rot in loblolly pine (Pinus taeda L.) unthinned plantations and subsoiled seed orchards and to demonstrate the potential for direct root colonization by percolated basidiospores. Twenty-two 0.02 ha circular plots were installed in nine unthinned loblolly pine plantations in Virginia. Five plantations were located on sites classified as high hazard for annosum root rot while four plantations were located on low hazard sites. Bulldozer excavation of the root systems permitted extensive analysis of annosum root rot incidence and severity which was substantiated by isolation of the asexual stage of the fungus from symptomatic resinous and stringy-decayed roots. On low hazard sites, 10 of 300 trees (3.3 percent) were colonized by H. annosum with 2 trees (0.7 percent) severely colonized ( > 1 percent of the total root system mass). On high hazard sites, 29 of 348 trees (8.3 percent) were colonized with 5 trees (1.4 percent) severely colonized. On low hazard sites, the predominant colonization symptom was stringy decay which indicated an older established disease situation as opposed to colonized trees on high hazard sites which exhibited resin-soaking characteristic of more recent infection and colonization. No basidiocarps were observed on any trees on low hazard plots while only 2 trees exhibited conks on high hazard sites. Mean radial increment growth differences between H. annosumcolonized and noncolonized trees were analyzed using the Duncan's Multiple Range test and for the 10-year period prior to excavation no significant growth reduction was observed. Due to low incidence and severity of annosum root rot in unthinned loblolly pine plantations and the absence of reduced radial increment growth, the disease is not a primary management consideration, especially on low hazard sites. However, the disease may be of secondary importance in managing first-generation unthinned loblolly pine plantations on high hazard sites due to inoculum production from residual stumps of H. annosum trees removed during thinning. The root systems of 2, 20 and 30 loblolly pines at the Chesapeake Corporation, Virginia Division of Forestry and Union Camp Corporation seed orchards, respectively, were excavated with a backhoe to permit intensive analysis of subsoiled roots for annosum root rot incidence and severity. The absence of wound callus, lack of adventitious root formation and the presence of a resin-soaked band greater than approximately 6 mm wide at the wound surface were three criteria for determining whether a lateral primary root had failed to heal following subsoiling. Soil texture was closely associated with the incidence and severity of general root disease as the wetter soil conditions at the Chesapeake Corporation seed orchard probably afforded a more amenable environment for the healing of subsoiled roots. Sandy, drought-susceptible soils at the Union Camp Corporation and Virginia Division of Forestry seed orchards were associated with the higher incidence and severity of root disease among subsoiled lateral primary roots. The asexual stage of H. annosum was not successfully isolated from resin soaked subsoiled root tissue at any of the three orchards. Duncan's Multiple Range analysis demonstrated that mean radial increment growth was significantly decreased among healthy and declining subsoiled trees at the three orchards. At the Union Camp Corporation orchard, when healthy and declining trees were subsoiled they exhibited significantly less radial increment growth than their respective control counterparts. Loblolly pine root segments were inoculated with a suspension of H. annosum basidiospores and observed using scanning electron microscopy. Appressorial-like structures occurred at distal ends of elongated germ tubes demonstrating probable direct infection of loblolly pine root segments. / Ph. D.

Understory forage production, species composition, and nutrient concentrations were studied in relation to overstory structure and site characteristics in thinned loblolly pine (Pinus taeda) plantations in the Virginia Piedmont. Stands exhibited a wide range in overstory basal areas (18.8 to 43.5 m²/ha) and site indices (14.2 to 23.8 m at base age 25 years). Understory forage production for the 0 to 2 m stratum averaged 610 kg/ha and ranged from 154 to 1690 kg/ha. Initially, differential models were used to develop prediction equations relating understory production to overstory characteristics, but an empirical prediction equation proved to be somewhat superior. Forage production was most predictable from total overstory basal area, canopy cover, and slope position. Understory species composition was analyzed with respect to overstory structural and site gradients, using vegetation ordination techniques. Species composition was less closely related to these gradients than was production; however, the greatest species diversity appeared to occur during the period following thinning but before crown closure, when successional and mid-tolerant species coexisted. Nutrient concentrations in forage material averaged 4643 cal/g for gross energy, 38.3 percent for invitro dry matter digestibility by white-tailed deer (Odoooiteus virginiana) and 8.4 percent for crude protein. There were no apparent trends of these nutrient concentrations with respect to overstory structural or site characteristics. Forage based carrying capacities for white-tailed deer were computed using values from this study for production and nutrient concentrations, and values from the literature for deer forage preferences and nutrient requirements. Results indicated that the pine habitat could support 0.03 to 0.19 lactating does per ha during the summer season, and that energy and not protein is likely the limiting nutritive parameter. Suggestions are made for future research in pine overstory-understory and wildlife habitat relationships. These include the use of experimental overstory manipulation followed by periodic remeasurements to directly observe changes in understory production and species composition, intensive sampling to determine specific local wildlife forage preferences, and the quantification of wildlife movements and population dynamics. / Ph. D.

Over the next few decades, timber harvest levels on the South's non-industrial private forestlands (NIPFs) are projected to increase by 40 percent. These ownerships include 75 percent of the commercial timberland in the South, and account for 64 percent of the timber volume harvested in the region annually. Long-term sustained yield of softwood timber volumes from NIPFs depends on successful reforestation of harvested pine stands and afforestation of marginal agricultural lands. Available technical assistance is regarded as a significant factor in NIPP tree planting accomplishment. This study compares technical assistance available to NIPFs from various sources and attempts to measure response to technical assistance in terms of acres planted. Four sources of technical assistance are considered: state forestry agencies, consulting foresters, industry landowner assistance departments, and industry procurement staffs. Extensive south-wide surveys of technical assistance available from these four sources were conducted to determine the types and degrees of assistance available. Cross-sectional comparisons were made between 29 sub-state zones in 12 southern states to explore variation in NIPF acres planted attributable to variation in technical assistance. The surveys located just under 2,000 foresters having some professional involvement with southern landowners in 1985. The number of consulting foresters operating in the South seems to be growing, while the number of landowners enrolled in industry landowner assistance programs (LAPs) is also increasing. Assistance available to NIPF owners from all sources ranges from advice and recommendations to actual performance of site preparation and tree planting. The types and intensities of technical assistance are variable both within and across forester types. A cross-sectional statistical analysis using multiple linear regression was unable to demonstrate a significant south-wide effect of technical assistance on NIPF tree planting. Within the range of technical assistance presently available, variation in technical assistance did not prove to be a significant predictor of tree planting accomplishment. The results of this study indicate that diversity in the NIPF population, combined with diversity in silvicultural and market potential pine production, prevents quantification of a regional effect of technical assistance. A positive correlation between forester numbers and NIPF tree planting accomplishments was found. But only timber harvest levels and cost share expenditures were significant predictors of acres planted in multiple variable models. / Master of Science

The objective of this study was to develop diameter/basal area increment equations for loblolly pine trees in thinned and unthinned plantations on cutover, site-prepared areas. Results indicated that one set of coefficients was sufficient to estimate individual tree growth (for the three year period following thinning) on lightly thinned and heavily thinned plots. However, unthinned plots required a separate set of coefficients and therefore a separate equation to estimate growth. Diameter growth was adequately explained by some form of the following regressor variables: pine basal area, hardwood basal area, initial age, initial diameter, average height of the dominant and codominant trees, and crown ratio ( optional). Transforming the dependent variable from a function of diameter to a similar function of basal area had no apparent effect on the precision of the predicted results. Two alternative methods of predicting diameter growth were evaluated: (1) direct fitting of diameter growth, and (2) fitting a potential diameter growth equation and a modifier function. Even though the potential times modifier approach performed slightly better in terms of fitting the data, it provided unrealistic results at ages beyond the upper range of the data. After additional data are obtained at older ages, the potential times modifier approach may surpass the direct approach. However, at present, the direct diameter growth model was chosen as the final model form. / M.S.

A loblolly pine phosphorus fertilization trial was evaluated 20 years after establishment on a Tatum silt loam in the Virginia Piedmont. Triple superphosphate (TSP) was applied at 160 kg P/ha and ground rock phosphate (GRP) was applied at both 160 kg P/ha and 670 kg P/ha. Lime (4.48 T/ha) was applied with and without the TSP treatment. Tree growth was not significantly affected by treatment and foliar phosphorus levels were above 0.10% indicating that a deficiency was not the immediate growth limiting factor. Double-acid-extractable soil phosphorus critical levels established for the Coastal Plain do not appear useful for diagnosing tree requirements for this Piedmont soil. A critical level of 1.0 ppm double-acid-extractable phosphorus would be more applicable to this soil. GRP was more effective than TSP after 20 years at increasing phosphorus uptake, probably due to a slower dissolution rate and the inclusion of F-ions which reacted with soil Al to reduce phosphorus fixation. Although an increase in the A horizon pH persisted for 20 years, there was no increase in phosphorus uptake as a direct response to this higher pH. Liming may have some long-term merit when applied in conjunction with a water soluble phosphorus fertilizer such as TSP by reducing the transformations of applied phosphorus to unavailable forms. / Master of Science

The longitudinal air permeabilities of the wood of 1116 specimens from 279 trees, two sapwood and two heartwood replicates, representing two varieties of lodgepole pine (Pinus contorta, vars. latifolia and murrayana) were measured with a steady state apparatus. It was found that the mean ratio of sapwood to heartwood permeability was ca. 10:1 for both varieties. The mean ratio of var. latifolia to murrayana was 1.5:5 and 1.75:5 for sapwood and heartwood, respectively. The most important source of variation following the difference between heartwood and sapwood was that among trees. Geographical locations, such as latitude and elevation did not significantly influence permeability. Tree size did, but only because the small trees (3 inch diameter) showed higher heartwood permeability and lower sapwood permeability than normal. Ca. 20 specimens of latifolia heartwood showed extremely high permeabilities. They were also deeply brown in color, which probably was caused by fungal or bacterial infestation. Pit pore size and number per cm² were determined for sapwood by making four permeability measurements, each at a different average pressure on each specimen. A mean pit pore radius of 1.5 µm and 1.3 µm for sapwood of var. latifolia and var. murrayana was calculated. The median values between 1200-1300 pit pores per cm² indicate an average rate of tracheid connection of 1.2-1.3%. Of the tested wood parameters including moisture content and specific gravity average ringwidth, only the permeability of var. latifolia was significantly correlated with moisture content for both heartwood and sapwood, with a negative correlation coefficient. Water retention measurements were carried out to relate the measured gas permeability of an individual specimen to its ability to absorb water. For both varieties the retention was significantly and quadratically correlated with sapwood permeability (R² = 0.286 and 0.224) and was linearly correlated with heartwood permeability (R² = 0.488 and 0.5775). The correlation factors for the regression between retention and the logarithm of permeability were 0.239 and 0.227 for sapwood and 0.447 and 0.420 for heartwood. / M.S.

Thirteen white pine plots, ranging from 15-26 years of age and one tenth acre in size, were sampled in six counties of southwestern Virginia. The number of trees attacked by the white-pine weevil ranged from 3.5 percent to 98.6 percent with an average of 40.0 percent. The incidence of forking was found to be 4.1 percent of the trees weevi1ed. Enough trees in the dominant and co-dominant crOvffi categories were either free of weevil attacks or only attacked once that 250 to 300 trees are available for final harvest. Most of the weevil attacks occurred between 5 to 12 years of age. Analysis of Covariance for non-weevi1ed terminals and lateral lengths developed the following relationship for three age classes of terminals and laterals. / Master of Science

Field and greenhouse studies were conducted to investigate the role of water stress and time of glyphosate spraying in the variation in glyphosate efficacy. Data on water potential, foliar sugar and starch content, weather, and growth response were gathered for loblolly pine and four of its major competitors on 16 operationally sprayed tracts in Virginia. Glyphosate successfully released loblolly pine on all tracts. Control of white oaks was significantly related to foliar sugar concentration. Water potential and weather variables were not related to glyphosate efficacy for any species. Seedlings of loblolly pine, red maple, and sweetgum were raised in a greenhouse and nursery environment. At the end of the second growing season, three water stress treatments were imposed on each species at each of four glyphosate application dates. ¹⁴C-glyphosate was applied to a subsample of seedlings. Timing of application. water stress, or both significantly affected susceptibility of all three species to glyphosate. Efficacy for all three species corresponded to that expected from field data. Differences in species susceptibility to glyphosate were explained by differences in ¹⁴C-glyphosate translocation, but there was no difference among species in absorption of glyphosate. Efficacy changes across application dates followed seasonal changes in foliar sugar concentration. / Master of Science