O presente estudo foi desenvolvido para testar a viabilidade do Bioensaio Trad- MCN com Tradescantia pallida cv. purpurea Boom em situações naturais, ou seja, plantada em diferentes tipos de solo em sete Parques Urbanos da cidade de São Paulo. Em cada Parque foi plantado um grupo de T. pallida na borda próxima a uma via de grande circulação de veículos e outro grupo de T. pallida no centro, com intuito de verificar possíveis gradientes de poluição, visto que o tráfego de veículos automotores é um dos principais responsáveis pela poluição do ar na cidade. Além da quantificação de micronúcleos em células-mãe de grão de pólen (Trad-MCN), foi analisado com a técnica EDX-RF o acúmulo de elementos traço nas folhas de T. pallida e no solo, tanto na borda e como no centro. Medidas de O3 e NO2 também foram realizadas nos diversos pontos amostrados. Foi verificada diferença estatística significante no Bioensaio de Trad-MCN entre borda e centro somente nos Parques Toronto t = 2,631; p = 0,020 e Guarapiranga t = 2,631; p = 0,019. O menor fluxo de veículos no entorno aliado a presença de grande extensão florestal no centro do Parque teve relação direta com as maiores médias registradas O3 que foi no Parque Guarapiranga. O intenso fluxo de veículos na região central da cidade pode ser um dos fatores para justificar altos índices de chumbo (Pb) acumulado, tanto nas folhas como no solo na borda do Parque Independência. Em conclusão o Bioensaio Trad-MCN em condições naturais necessita maiores estudos para verificar se além da poluição do ar, as condições de estresse climático podem induzir mutações genéticas em T. pallida, e as análises de elementos traços com técnica de EDX-RF mostraram presença de diversos elementos traços registrados nas folhas, sendo alguns deles com maior concentração na borda em relação ao centro dos parques estudados. / This current study was designed to test the viability of the Trad-MCN bioassay with Tradescantia pallida cv. purpurea Boom in natural situations, planted in different soil types in seven urban parks in the city of São Paulo. In each Park one group of T. pallida was planted on fringe, next to a path of movement of large vehicles, and another group of T. pallida in the center, aiming to investigate possible gradients of pollution, since the traffic of motor vehicles is a major driver of air pollution in the cities. Besides the quantification of micronuclei in mother cells of pollen grain (Trad-MCN), was analyzed with EDX-RF technique the accumulation of trace elements in the leaves of T. pallida and the soil, as both the fringe and the center. Measurements of O3 and NO2 were also performed in the various sampling points. Statistically significant difference was found in the Trad-MCN bioassay between fringe and center only in Toronto Park t = 2.631; p = 0.020 and Guarapiranga Park t = 2.631; p = .019. The highest averages recorded of O3 was found in the center of Chico Mendes Park, the lowest flow of vehicles in the vicinity ally the presence of large forest extension in the center of the park had a direct relationship with the major averages recorded O3. The heavy flow of vehicles in the central city can be a factor to justify high levels of lead (Pb) accumulated in both the leaves and soil at the edge of Independência Park. In conclusion, the Trad-MCN bioassay in natural conditions requires further studies to determine whether addition of air pollution, the conditions of environmental stress can induce genetic mutations in T. pallida, and the analysis of trace elements with EDX-RF technique showed the presence of several trace elements reported in the leaves and in soil, some of them with the highest concentration at the fringe from the center of the parks studied.

Aujourd'hui, l'environnement sonore n'est plus uniquement considéré comme une gène ou une nuisance par notre société, mais comme pouvant être porteur de qualités. La directive européenne 2002/45/CE,relative à la gestion du bruit dans l'environnement demande aux états d'identifier les «zones calmes» en vue de leur préservation et de leur valorisation en raison de la ressource qu'elles constituent. Elle présente les «zones calmes» comme des zones préservées du bruit. Dans ce contexte, les parcs et les jardins apparaissent dans de nombreuses études comme étant plébiscités par les usagers pour la qualité de leur environnement sonore sans que ces qualités ne soient bien identifiées. Si la directive européenne tend à considérer la qualité sonore en fonction d'un seuil de niveau sonore et des seules notions d'agrément ou de désagrément, les différents champs de recherche sur l'environnement sonore, de la psychoacoustique jusqu'aux ambiances,on montré qu'elle relève d'une expérience esthétique plus complexe. L'environnement sonore est chargé de valeurs affectives, sémantiques, fonctionnelles, esthétiques.Nous proposons ici une identification précise des qualités sonores qui semblent propres au parcs et aux jardins urbains en abordant la qualité sonore dans une perspective plus descriptive qu'hédoniste à travers l'étude de l'expérience vécue des usagers dans six parcs et jardins de la région parisienne. Dans un premier temps nous présentons une description des différentes facettes de l'environnement sonore du jardin établie à partir d'observations de terrains, de mesures acoustiques, et d'entretiens réalisés auprès des usagers. L'environnement sonore du jardin est présenté à partir de ses configurations sonores, d'effets perceptifs,des représentations qu'il suscite, ou encore des pratiques qu'il induit ou qui participent à son actualisation. Pour aller au delà des relations entre niveau sonore et agrément, nous proposons dans un second temps de révéler des ponts entre les aspects matériels et immatériels des ambiances sonores. Bien que les ambiances sonores relèvent d'une dimension immatérielle : le son et l'expérience esthétique des usagers, nous faisons l'hypothèse qu'elles peuvent être manipulables par les aménageurs ou faire l'objet de mesures de protection ou de valorisation si elles sont considérées comme des effets de dimensions physiques observables : structures paysagères ou indicateurs acoustiques. Nous abordons ainsi la qualité sonore à travers le prisme de la diversité architecturale et conceptuelle propre à l'art des jardins et nous proposons de comprendre les relations entre les différentes qualités sonore identifiées, les structures paysagères et l'acoustique des jardins. / Nowadays, the sound environment is no longer only considered as potential annoyance, but it is sometimes recognize for the qualities it can bear. The European directive 2002/45/CE, relative to the management of environmental noise, requires the european states to identify «quiet areas» for their preservation and valorization due to the resource they represent. It defines the «quiet areas» as areas preserved from the noise. In this context, numerous studies have shown that in the urban environments the parks and gardens are acclaimed by users for the quality of their soundscape, but those qualities have never been accurately identified. If the European directive seems to consider the sound quality according to a noise level and the ideas of agreement or disagreement, different fields of study on the sound environment, from the psychoacoustic to the atmosphere studies, have shown that the esthetic experience is way more complex. The sound environment is full of emotional values, semantic, functional and aesthetic values.The aim of our work is to accurately identify the sound qualities that seem specific to urban parks and gardens. The sound qualities are understood more from a descriptive than an hedonist point of view, through the study of the experience of users in six differents parks and gardens of the Parisan area. First, it present a description of the different aspects of the sound environment of the garden established from various kind of datas : observations, acoustic measurements, and interviews with users. The sound environment of the garden is presented through sound configurations, perceptual effects, representations it evokes, or through practices. To overcome the relationship between noise level and agreement, this work propose in a second time to highlight links between tangibles and intangibles aspects of the soundscape. Although the soundscape seems to fall within an immaterial dimension : sound and aesthetic experience of the users, we assume that it can be mastered by the designers, the acousticians or the urban planners if it is considered as an effect of material dimensions : landscape structures or acoustic indicators. This work approach the sound quality through the architectural diversity of the garden design. It propose to understand the relationships between the different sound qualities identified, the landscape structures and the acoustic measurments.

O presente estudo foi desenvolvido para testar a viabilidade do Bioensaio Trad- MCN com Tradescantia pallida cv. purpurea Boom em situações naturais, ou seja, plantada em diferentes tipos de solo em sete Parques Urbanos da cidade de São Paulo. Em cada Parque foi plantado um grupo de T. pallida na borda próxima a uma via de grande circulação de veículos e outro grupo de T. pallida no centro, com intuito de verificar possíveis gradientes de poluição, visto que o tráfego de veículos automotores é um dos principais responsáveis pela poluição do ar na cidade. Além da quantificação de micronúcleos em células-mãe de grão de pólen (Trad-MCN), foi analisado com a técnica EDX-RF o acúmulo de elementos traço nas folhas de T. pallida e no solo, tanto na borda e como no centro. Medidas de O3 e NO2 também foram realizadas nos diversos pontos amostrados. Foi verificada diferença estatística significante no Bioensaio de Trad-MCN entre borda e centro somente nos Parques Toronto t = 2,631; p = 0,020 e Guarapiranga t = 2,631; p = 0,019. O menor fluxo de veículos no entorno aliado a presença de grande extensão florestal no centro do Parque teve relação direta com as maiores médias registradas O3 que foi no Parque Guarapiranga. O intenso fluxo de veículos na região central da cidade pode ser um dos fatores para justificar altos índices de chumbo (Pb) acumulado, tanto nas folhas como no solo na borda do Parque Independência. Em conclusão o Bioensaio Trad-MCN em condições naturais necessita maiores estudos para verificar se além da poluição do ar, as condições de estresse climático podem induzir mutações genéticas em T. pallida, e as análises de elementos traços com técnica de EDX-RF mostraram presença de diversos elementos traços registrados nas folhas, sendo alguns deles com maior concentração na borda em relação ao centro dos parques estudados. / This current study was designed to test the viability of the Trad-MCN bioassay with Tradescantia pallida cv. purpurea Boom in natural situations, planted in different soil types in seven urban parks in the city of São Paulo. In each Park one group of T. pallida was planted on fringe, next to a path of movement of large vehicles, and another group of T. pallida in the center, aiming to investigate possible gradients of pollution, since the traffic of motor vehicles is a major driver of air pollution in the cities. Besides the quantification of micronuclei in mother cells of pollen grain (Trad-MCN), was analyzed with EDX-RF technique the accumulation of trace elements in the leaves of T. pallida and the soil, as both the fringe and the center. Measurements of O3 and NO2 were also performed in the various sampling points. Statistically significant difference was found in the Trad-MCN bioassay between fringe and center only in Toronto Park t = 2.631; p = 0.020 and Guarapiranga Park t = 2.631; p = .019. The highest averages recorded of O3 was found in the center of Chico Mendes Park, the lowest flow of vehicles in the vicinity ally the presence of large forest extension in the center of the park had a direct relationship with the major averages recorded O3. The heavy flow of vehicles in the central city can be a factor to justify high levels of lead (Pb) accumulated in both the leaves and soil at the edge of Independência Park. In conclusion, the Trad-MCN bioassay in natural conditions requires further studies to determine whether addition of air pollution, the conditions of environmental stress can induce genetic mutations in T. pallida, and the analysis of trace elements with EDX-RF technique showed the presence of several trace elements reported in the leaves and in soil, some of them with the highest concentration at the fringe from the center of the parks studied.