Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-25T22:57:37Z No. of bitstreams: 1 AndreaCarolineCostaDeArruda_DISSERT.pdf: 2892778 bytes, checksum: ab6349b021e0a01fec34dc8730e5aa85 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-27T22:19:59Z (GMT) No. of bitstreams: 1 AndreaCarolineCostaDeArruda_DISSERT.pdf: 2892778 bytes, checksum: ab6349b021e0a01fec34dc8730e5aa85 (MD5) / Made available in DSpace on 2016-04-27T22:19:59Z (GMT). No. of bitstreams: 1 AndreaCarolineCostaDeArruda_DISSERT.pdf: 2892778 bytes, checksum: ab6349b021e0a01fec34dc8730e5aa85 (MD5) Previous issue date: 2015-07-20 / O pericarpo de Passiflora edulis var. flavicarpa Degener atualmente est? sendo investigado para fins medicinais. N?o h? relatos na literatura sobre sua toxicidade. O objetivo do presente estudo foi investigar a toxicidade sub-cr?nica em ratos machos e toxicidade em ratas f?meas prenhes e fetos expostos durante a gesta??o a um extrato obtido por infus?o do pericarpo em ?gua (1:3 m/v; 100o C, 10 min). A composi??o do extrato foi avaliada por meio de rea??es qu?micas e tamb?m por cromatografia de camada delgada. Ratos machos adultos (n=8) foram tratados com 300 mg/kg de extrato, por gavagem, durante 30 dias e ratas prenhes (n=7) do dia 0 ao dia 20 de gesta??o. Os grupos controle de ratos machos (n=8) e ratas f?meas (n=7) receberam ?gua pot?vel (1 ml). Durante o tratamento foram registrados o consumo de ?gua e de ra??o e o peso corporal. No dia 29 o comportamento sexual dos machos foi analisado e, em seguida, metade dos machos de cada grupo receberam ciclofosfamida (50 mg/kg, i.p.) para avalia??o (anti) genot?xica na medula ?ssea e a outra metade recebeu apenas solu??o salina 0,9% (1 mL, i.p.). No dia 30, os machos foram anestesiados para coleta de sangue, medula ?ssea e ?rg?os. No dia 20 de gesta??o, as ratas prenhes foram anestesiadas para avalia??o do desempenho reprodutivo. A an?lise dos fetos foi realizada por exame visceral e esquel?tico. A an?lise fitoqu?mica revelou a presen?a de flavon?ides, alcal?ides inespec?ficos, compostos fen?licos e triterp?nicos. A an?lise estat?stica revelou aus?ncia de diferen?as em todos os par?metros analisados para machos, para f?meas e para fetos experimentais em rela??o aos animais controle. A citotoxicidade foi avaliada pela rela??o entre eritr?citos policrom?ticos e normocrom?ticos (EPC/ENC). A an?lise estat?stica (m?dia ? erro do desvio padr?o) revelou aus?ncia de altera??es na frequ?ncia de EPCMN (controle negativo: 3,26?0,42; controle positivo: 11,72?1,02; experimental negativo: 4,02?0,13; experimental positivo: 10,47?0,87) ou citotoxicidade (controle negativo: 0,37?0,08; controle positivo: 0,23?0,05; experimental negativo: 0,37?0,07; experimental positivo: 0,23?0,02). Este estudo sugere que o extrato aquoso obtido de pericarpo de P. edulis var. flavicarpa Degener n?o foi capaz de promover efeitos t?xicos em ratos nas condi??es utilizadas. / The pericarp of Passiflora edulis var. flavicarpa Degener is now being investigated for medicine purposes. There are no reports about it toxicity. The aim of the present study was investigate the sub chronic toxicity in male rats and reproductive toxicity in pregnant rats and exposed fetuses of an extract obtained by infusion of the pericarp in water (1:3 m/v;100o C, 10 min). The extract composition was evaluated by tube reactions and thin lawyer chromatography (TLC). Adult male rats (n=8) were treated with 300 mg/kg of the extract, by gavage, during 30 days and pregnant rats (n=7) from gestation day 0 to day 20. Control received tap water (1 mL). Water and food intakes and body weight gain were recorded. At day 29 of treatment the sexual behavior of the males was analyzed and then half of males from each group received cyclophosphamide (50 mg/kg, i.p.) to (anti)genotoxic assessment in bone marrow. At day 30, males were anesthesized for parameters collection. At day 20 of gestation, the dams were anesthesized for reproductive performance evaluation. The fetal analysis was conducted by visceral and skeletal. Phytochemical analysis revealed the presence of flavonoids, unspecific alkaloids, phenols and triterpenic compounds. Statistical analysis revealed absence of significant differences between experimental and control. This study suggest that the aqueous extract obtained from pericarp of P. edulis var. flavicarpa Degener was not able to promote toxic effects in rats. Cytotoxicity was evaluated with the PCE/NCE ratio (NCE=normochromatic erythrocytes). Statistical analysis (mean ? SEM) revealed absence of changes in the frequency of MNPCE (negative control: 3.26?0.42; positive control: 11.72?1.02; negative experimental: 4.02?0.13; positive experimental: 10.47?0.87) or cytotoxicity (negative control: 0.37?0.08; positive control: 0.23?0.05; negative experimental: 0.37?0.07; positive experimental: 0.23?0.02). This study suggests that the extracts showed no (anti)genotoxic and no cytotoxic activities under the experimental conditions.