In our current environment, where we increasingly become dependent on the internet, the definition of living smart is widely used. Smart living involves original and innovative solutions to make life more efficient, controllable, economical, productive, integrated and sustainable. The Internet of Things (IoT) helps people with the lifestyle of smart living and helps them gain complete control. IoT does not only offer smart devices for the home, such as Google Home or Alexa. It is also essential for small and big businesses. The ability to transfer data over a network without any human interaction is needed in today's society. Organisations in multiple industries are using IoT to improve decision-making and overall quality of life for their employees. One way of using the IoT infrastructures is a concept called digital twin or a Digital Twin of an Organization (DTO). Since an organisation is constantly evolving, keeping the DTO aligned with the organisation will require an infrastructure that lends itself to be highly flexible and maintainable. The EA Blueprint Pattern was proposed to help achieve a flexible and maintainableDTO. However, there are limited practical instantiations of this pattern that can evaluate its utility. This project will therefore perform a practical evaluation and analysis of the pattern.

Microscopic techniques with high spatial and temporal resolution are required for in vivo studying biological cells and tissues. Adaptive lenses exhibit strong potential for fast motion-free axial scanning. However, they also lead to a degradation of the achievable resolution because of aberrations. This hurdle can be overcome by digital optical technologies. We present a novel High-and-Low-frequency (HiLo) 3D-microscope using structured illumination and an adaptive lens. Uniform illumination is used to obtain optical sectioning for the high-frequency (Hi) components of the image, and nonuniform illumination is needed to obtain optical sectioning for the low-frequency (Lo) components of the image. Nonuniform illumination is provided by a multimode fiber. It ensures robustness against optical aberrations of the adaptive lens. The depth-of-field of our microscope can be adjusted a-posteriori by computational optics. It enables to create flexible scans, which compensate for irregular axial measurement positions. The adaptive HiLo 3D-microscope provides an axial scanning range of 1 mm with an axial resolution of about 4 microns and sub-micron lateral resolution over the full scanning range. In result, volumetric measurements with high temporal and spatial resolution are provided. Demonstration measurements of zebrafish embryos with reporter gene-driven fluorescence in the thyroid gland are presented.