The Matlabas mire, an actively peat accumulating wetland, is located in the headwaters of the Matlabas River, Marakele National Park, Limpopo Province, South Africa. Various seepage zones and artesian peat domes are contained in this peatland that consists of two tributaries of which the western one is partially channelled. The occurrence of decaying peat domes and desiccated areas with terrestrial vegetation, as well as the apparent erosion on the western tributary, have raised concerns on the health of this wetland. A network of piezometers was installed in the mire and results confirm that the system is fed primarily from seepage from the slopes of the catchment. Chemical analysis and temperature recorded indicate an isolated groundwater source of which the water does not mix with surface water. This is linked with isotope analysis of the age of peat in various sections of the mire. Erosion was attributed to anthropogenic changes in the catchment. Management recommendations include rehabilitation and reinstating the driving forces that support the mire. / Environmental Sciences / M. Sc. (Environmental Management)