The purpose of this explanatory sequential mixed methods study was fourfold: examine the pre-service science teachers' pedagogical orientations about science teaching; examine the pre-service science teachers' beliefs about science teaching; establish the extent to which the pre-service science teachers' pedagogical orientations correlate with their beliefs; and establish factors that shape the pedagogical orientations and beliefs of pre-service science teachers. A sample comprised 140 secondary school pre-service science teachers at three universities in Zambia. There were 102 males and 38 females. In the first phase, data were collected using the Pedagogy of Science Teaching Test (POSTT) that was adapted to suit the Zambian curriculum, and the Teacher Beliefs Interview (TBI) protocol. Whereas, in the second phase, a semi-structured interview was used to collect data. Twelve pre-service science teachers were interviewed to solicit in-depth information on the findings from the POSTT and TBI protocol.