Copies of author's previously published articles inserted. Bibliography: leaves 175-194. Eikenella corrodens, a Gram-negative rod, is a normal inhabitant of the human oral cavity. It is one of the most commonly detected cultivable bacteria from sub- and supra-gingival plaque and is often isolated in elevated proportions from sites exhibiting destructive periodontal disease compared with healthy sites. The primary aim of this study was to investigate the metabolism of E. corrodons, with particular reference to energy generation, and to determine the effect of physical environmental parameters on its growth.

Copies of author's previously published articles inserted. / Bibliography: leaves 175-194. / xiii, 195 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Eikenella corrodens, a Gram-negative rod, is a normal inhabitant of the human oral cavity. It is one of the most commonly detected cultivable bacteria from sub- and supra-gingival plaque and is often isolated in elevated proportions from sites exhibiting destructive periodontal disease compared with healthy sites. The primary aim of this study was to investigate the metabolism of E. corrodons, with particular reference to energy generation, and to determine the effect of physical environmental parameters on its growth. / Thesis (Ph.D.)--University of Adelaide, Dept. of Dentistry, 2000

Copies of author's previously published articles inserted. / Bibliography: leaves 175-194. / xiii, 195 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Eikenella corrodens, a Gram-negative rod, is a normal inhabitant of the human oral cavity. It is one of the most commonly detected cultivable bacteria from sub- and supra-gingival plaque and is often isolated in elevated proportions from sites exhibiting destructive periodontal disease compared with healthy sites. The primary aim of this study was to investigate the metabolism of E. corrodons, with particular reference to energy generation, and to determine the effect of physical environmental parameters on its growth. / Thesis (Ph.D.)--University of Adelaide, Dept. of Dentistry, 2000

Resumo: Objetivos: Dois estudos controlados cegos aleatórios foram realizados para avaliar os resultados clínicos e microbiológicos iniciais e a longo prazo, da tetraciclina aplicada com o microbrush no tratamento de sítios com bolsa periodontal persistente 5mm e sangramento à sondagem, tratados previamente por raspagem e alisamento radicular (RAR). Material e método: Quarenta e dois pacientes com pelo menos 4 dentes com bolsas periodontais 5 mm e sangramento à sondagem foram divididos em 2 grandes grupos: tetraciclina e controle. Nos grupos tetraciclina, dois dentes receberam aplicação de solução de cloridrato de tetraciclina (100mg/mL) com microbrush uma vez por semana por 4 semanas (grupo T) e os outros dois dentes receberam o mesmo tratamento associado a uma sessão de RAR na primeira consulta (grupo RAR+T). Nos grupos controle, dois dentes receberam uma sessão de RAR (grupo RAR) e outros dois dentes receberam aplicação de solução salina com microbrush uma vez por semana por 4 semanas associada a uma sessão de RAR na primeira consulta (grupo RAR+S). Medidas clínicas de índice de placa visível (IP), índice de sangramento gengival (IG), sangramento à sondagem (SS), profundidade de sondagem (PS), nível de inserção clínica (NIC), recessão gengival (RG), e coletas microbiológicas para identificação das bactérias Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas gingivais, Prevotella intermédia, Prevotella nigrescens, Tannerella forsythia, e Treponema denticola foram tomadas no baseline, 30, 90 e 180 dias após o tratamento. Amostras de biofilme subgengival foram analisadas por reação de polimerase em cadeia. Resultados: Todas as terapias resultaram em melhora na PS, NIC e SS, embora as diferenças clínicas entre os grupos não tenham alcançado significância estatística. (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Objectives: Two randomized controlled single-blind studies were carried out to evaluate the initial and the long term clinical and microbiological results of tetracycline applied with a microbrush in treatment of sites with persistent periodontal pockets 5mm and bleeding on probing previous treated by scaling and root planning (SRP). Methods: For each patient in the test groups, two teeth received 4 applications of tetracycline solution (100mg/ml) with a microbrush once a week (T group) while the other two teeth received the same treatment plus one session of SRP at the first appointment (SRP+T group). For each patient in the control groups, two teeth received one session of SRP (SRP group) and the other two received 4 applications of saline with a microbrush once a week plus one session of SRP (SRP+S group). Clinical measurements were taken at baseline, 30, 90 and 180 days after last treatment. Subgingival biofilme samples were analyzed by polymerase chain reaction for Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas gingivais, Prevotella intermédia, Prevotella nigrescens, Tannerella forsythia, e Treponema denticola identification. Results: All therapies provided significant improvement in probing depth, clinical attachment level and bleeding on probing, although the clinical difference did not reach statistically significance. At 1 month post-therapy the prevalence of P. gingivalis was significantly reduced in groups that received SRP plus additional therapy and T. forsythia was significantly reduced in only test groups. Until 6 months P. gingivalis, T. forsythensis,and A. actinomycetemcomitans significantly decrease in prevalence only for tetracycline groups. Conclusion: The results of the present study suggest that tetracycline solution applied with a microbrush may be an alternative in management of persistent periodontal pockets with further microbiologic benefits. / Orientador: Rosemary Adriana Chiérici Marcantonio / Coorientador: Denise Madalena Palomari Spolidorio / Banca: Adriana Campos Passanezi Sant'Ana / Banca: José Roberto Cortelli / Banca: Sérgio Luiz de Souza Salvador / Banca: Silvana Regina Perez Orrico / Doutor

Bibliography: leaves 107-138.

Made available in DSpace on 2014-06-11T19:33:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-12Bitstream added on 2014-06-13T18:45:12Z : No. of bitstreams: 1 bosco_jmd_dr_arafo.pdf: 381181 bytes, checksum: 5b8b6929d9ffb9e26d5e951e6958d4a3 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Objetivos: Dois estudos controlados cegos aleatórios foram realizados para avaliar os resultados clínicos e microbiológicos iniciais e a longo prazo, da tetraciclina aplicada com o microbrush no tratamento de sítios com bolsa periodontal persistente 5mm e sangramento à sondagem, tratados previamente por raspagem e alisamento radicular (RAR). Material e método: Quarenta e dois pacientes com pelo menos 4 dentes com bolsas periodontais 5 mm e sangramento à sondagem foram divididos em 2 grandes grupos: tetraciclina e controle. Nos grupos tetraciclina, dois dentes receberam aplicação de solução de cloridrato de tetraciclina (100mg/mL) com microbrush uma vez por semana por 4 semanas (grupo T) e os outros dois dentes receberam o mesmo tratamento associado a uma sessão de RAR na primeira consulta (grupo RAR+T). Nos grupos controle, dois dentes receberam uma sessão de RAR (grupo RAR) e outros dois dentes receberam aplicação de solução salina com microbrush uma vez por semana por 4 semanas associada a uma sessão de RAR na primeira consulta (grupo RAR+S). Medidas clínicas de índice de placa visível (IP), índice de sangramento gengival (IG), sangramento à sondagem (SS), profundidade de sondagem (PS), nível de inserção clínica (NIC), recessão gengival (RG), e coletas microbiológicas para identificação das bactérias Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas gingivais, Prevotella intermédia, Prevotella nigrescens, Tannerella forsythia, e Treponema denticola foram tomadas no baseline, 30, 90 e 180 dias após o tratamento. Amostras de biofilme subgengival foram analisadas por reação de polimerase em cadeia. Resultados: Todas as terapias resultaram em melhora na PS, NIC e SS, embora as diferenças clínicas entre os grupos não tenham alcançado significância estatística. / Objectives: Two randomized controlled single-blind studies were carried out to evaluate the initial and the long term clinical and microbiological results of tetracycline applied with a microbrush in treatment of sites with persistent periodontal pockets 5mm and bleeding on probing previous treated by scaling and root planning (SRP). Methods: For each patient in the test groups, two teeth received 4 applications of tetracycline solution (100mg/ml) with a microbrush once a week (T group) while the other two teeth received the same treatment plus one session of SRP at the first appointment (SRP+T group). For each patient in the control groups, two teeth received one session of SRP (SRP group) and the other two received 4 applications of saline with a microbrush once a week plus one session of SRP (SRP+S group). Clinical measurements were taken at baseline, 30, 90 and 180 days after last treatment. Subgingival biofilme samples were analyzed by polymerase chain reaction for Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans), Porphyromonas gingivais, Prevotella intermédia, Prevotella nigrescens, Tannerella forsythia, e Treponema denticola identification. Results: All therapies provided significant improvement in probing depth, clinical attachment level and bleeding on probing, although the clinical difference did not reach statistically significance. At 1 month post-therapy the prevalence of P. gingivalis was significantly reduced in groups that received SRP plus additional therapy and T. forsythia was significantly reduced in only test groups. Until 6 months P. gingivalis, T. forsythensis,and A. actinomycetemcomitans significantly decrease in prevalence only for tetracycline groups. Conclusion: The results of the present study suggest that tetracycline solution applied with a microbrush may be an alternative in management of persistent periodontal pockets with further microbiologic benefits.