Bibliography: leaves 97-114. "Self-incompatibility (SI) is an important genetic mechnism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal transduction."

Bibliography: leaves 97-114. Electronic publication: Fulltext available in PDF format; abstract in HTML format. "Self-incompatibility (SI) is an important genetic mechnism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal transduction." Electronic reproduction.[Australia] :Australian Digital Theses Program,2001.

Bibliography: leaves 116-144. In Phalaris coerulescens, gametophytic self-incompatibility is under the control of two unlinked genes, S and Z. An incompatible reaction occurs when both S and Z alleles of the pollen are present in the genotype of the recipient stigma. Either pollen grains fail to germinate or pollen tube growth is arrested shortly after contact with the stigma. This study examines the contribution of genes specifically expressed in the male gametophyte to the physiological processes during pollen tube germination and pollen stigma interaction. Among the aims of this study is the isolation of the Z gene.

Bibliography: leaves 116-144. / 144 leaves, [20] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / In Phalaris coerulescens, gametophytic self-incompatibility is under the control of two unlinked genes, S and Z. An incompatible reaction occurs when both S and Z alleles of the pollen are present in the genotype of the recipient stigma. Either pollen grains fail to germinate or pollen tube growth is arrested shortly after contact with the stigma. This study examines the contribution of genes specifically expressed in the male gametophyte to the physiological processes during pollen tube germination and pollen stigma interaction. Among the aims of this study is the isolation of the Z gene. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Sciences, 1996

Typewritten copy Comparative study of genes for resistance to bunt (Tilletia caries (D.C.) Tul. and T. foetida (Wallr.) Liro); Cytological investigations in Phalaris called part 2.

Self-incompatibility (SI) is an important genetic mechanism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal transduction. During evolution, several SI systems have been evolved. A unique SI system widely spreads in the grasses. In the grasses, two unlinked, multi-allelic loci (S and Z) determine SI specificity. A putative self-incompatibility gene (Bm2) was previously cloned. In this study, the role of Bm2 in self-incompatibility was investigated first. The cDNA homologues of Bm2 were sequenced from two pollen-only mutants. The results indicated that Bm2 is not the one of SI genes in Phalaris, but represents a subclass of thioredoxin h. Thus a map-based cloning strategy was then adopted to clone the SI genes from Phalaris. Fine linkage maps of the S and Z regions were constructed. RFLP probes from wheat, barley, oat and rye were screened and the S locus was delimited to 0.26 cM and the Z locus to 1.0 cM from one side using specially designed segregating populations. The S locus was located to the sub-centromere region of triticeae chromosome group 1 and the Z locus to the middle of the long arm of group 2. Finally, barley and rice bacterial artificial chromosome (BAC) clones corresponding to the S and Z region were identified to analyse the chromosome structures and to seek candidate SI genes. The abundant repetitive sequences in the identified barley BAC clones limit their usefulness. Identification of Rice BAC clones orthologous to the S and Z regions open the gate to use rice genome information to clone SI genes from the grasses. A positive rice clone (139.9 kb) orthologous to the S region contained 19 predicted genes. Several of these genes might be involved in pollen tube germination and pollen-stigma interaction, which are the major parts of SI reaction. A positive clone (118.9 kb) orthologous to the Z region gave 16 predicted genes. The predicted genes on the outmost ends of these clones could be used to construct contigs to cover the S and Z regions and delimit the S and Z loci in the grasses. / Thesis (Ph.D.)--Department of Plant Science, 2001.

Self-incompatibility (SI) is an important genetic mechanism to prevent the inbreeding of flowering plants and also an excellent system for studying cell-cell recognition and signal transduction. During evolution, several SI systems have been evolved. A unique SI system widely spreads in the grasses. In the grasses, two unlinked, multi-allelic loci (S and Z) determine SI specificity. A putative self-incompatibility gene (Bm2) was previously cloned. In this study, the role of Bm2 in self-incompatibility was investigated first. The cDNA homologues of Bm2 were sequenced from two pollen-only mutants. The results indicated that Bm2 is not the one of SI genes in Phalaris, but represents a subclass of thioredoxin h. Thus a map-based cloning strategy was then adopted to clone the SI genes from Phalaris. Fine linkage maps of the S and Z regions were constructed. RFLP probes from wheat, barley, oat and rye were screened and the S locus was delimited to 0.26 cM and the Z locus to 1.0 cM from one side using specially designed segregating populations. The S locus was located to the sub-centromere region of triticeae chromosome group 1 and the Z locus to the middle of the long arm of group 2. Finally, barley and rice bacterial artificial chromosome (BAC) clones corresponding to the S and Z region were identified to analyse the chromosome structures and to seek candidate SI genes. The abundant repetitive sequences in the identified barley BAC clones limit their usefulness. Identification of Rice BAC clones orthologous to the S and Z regions open the gate to use rice genome information to clone SI genes from the grasses. A positive rice clone (139.9 kb) orthologous to the S region contained 19 predicted genes. Several of these genes might be involved in pollen tube germination and pollen-stigma interaction, which are the major parts of SI reaction. A positive clone (118.9 kb) orthologous to the Z region gave 16 predicted genes. The predicted genes on the outmost ends of these clones could be used to construct contigs to cover the S and Z regions and delimit the S and Z loci in the grasses. / Thesis (Ph.D.)--Department of Plant Science, 2001.