>Magister Scientiae - MSc / Diabetes Mellitus is a major global health issue, affecting over 463 million adults in the world. Metformin is the standard drug administered to most people suffering fromdiabetes; however, this medication is contraindicated in many individuals, like most of the medicines developed to combat diabetes. Many diabetic patients turn to herbal medicines due to their renowned traditional use and fewer side effects.While the beneficial effects of phytotherapy are very evident, separation of nontoxic from toxic phytochemicals is still a challenge. Phytonanotherapy is a branch in nanotechnology that seeks to find the middle ground between the fast-acting mechanism of conventional drugs which also present with long lasting or severe toxic side effects, and the slowacting mechanism of phytotherapy which presents with less severe side effects. As such, the aim of this study was to pioneer the investigation of gold nanoparticles biosynthesized using the Carprobrotus edulis fruit aqueous extract (CeFe-AuNPs) as potential treatment for diabetes mellitus. Previously optimized conditions were used to synthesize CeFe-AuNPs which were concurrently characterized using UV-Vis, dynamic light scattering, High Resolution – Transmission Electron Microscopy and Fourier Transform Infrared Spectroscopy techniques. The physichochemical stability of CeFe-AuNPs in phosphate buffer saline, 0.5 % bovine serum albumin, water and 10 % NaCl was also investigated. The effect of CeFe and CeFe-AuNPs on glucose uptake by yeast cells was investigated using 5, 10 and 25 mM glucose reactions. Acute toxicity of CeFe and CeFe- AuNPs was conducted in female Wistar rats (n = 20) and major organs were analyzed through the haematoxylin-eosin stain. The anti-diabetic effects of the CeFe (200 and 400 mg/kg) and CeFe- AuNPs (100 and 200 mg/kg) were investigated in male Wistar rats divided into seven group (n = 6). Histopathology of the pancreas, and the serum insulin were determined.