Plankton metabolic balance (PMBm) of the surface mixed layer was calculated as the ratio of areal rates of gross photosynthesis (AGP) to community respiration (AR), and estimated for four Laurentian Great Lakes coastal sites of contrasting physical, optical and nutrient regime: western Lake Ontario, Hamilton Harbour, Georgian Bay and Woods Bay. The applied methods were the oxygen light-and-dark bottle and 14C bottle methods as well as the oxygen stable isotope method (18O method). PMBm was net autotrophic in most of the cases (73% of the observations). Within- and inter-system variations in PMBm were heavily dependent on both a ratio of light-saturated photosynthesis to community respiration (Pmax/R) and a ratio of euphotic to mixing depths (Zeu/Zm). While short-term within-system variations in PMBm were driven by the interplay of chlorophyll a (Chl a), total phosphorus (TP) and Zeu/Zm ratio, its inter-lake long-term variability had a different behaviour. Average ratios of AGP/AR were dependent only on DOC or single physical parameters such as Zeu or Zm, while PMBm determined as the ratio between average AGP and AR was controlled by the joint effect of DOC, TP and Chl a. DOC affected average AGP/AR ratios primarily via its control over fluctuations of the physical environment and had a depressing effect on AGP rates but did not control rates of AR. Independent measurements of volumetric rates of photosynthesis (P) and community respiration (R) were made by 18O method adjusted for wind-driven gas exchange and compared against estimates from bottle estimates. The 18O method in Lake Ontario gave internally inconsistent results (e.g. negative absolute rates of P and R) and poor agreement with independent estimates of P, R and P/R despite superficially plausible estimates for P/R. The low productivity of Lake Ontario and frequent disturbances of water column masked the biological signal in both DO abundance and its isotopic signature, and thus invalidated the assumptions of steady state conditions. However, in Hamilton Harbour and some other relatively sheltered sites that were sampled occasionally, 18O method predicted absolute rates of P that were well correlated well with bottle estimates. Isotope model estimates for R and P/R in the harbour were not well correlated with bottle estimates but were of comparable magnitude on average, and differences were explicable in terms of physical forces and the different time scales of response for the two methods. The Hamilton Harbour hypolimnion presented an anomalous behavior in oxygen stable isotopes (18O depletion) where seasonal development of DO depletion was not accompanied by the progressive isotope enrichment expected from respiratory fractionation. The Lake Ontario and harbour hypolimnion results both appear to show that simple steady state models that assume literature values for fractionation processes and ignore physical dynamics are of limited applicability to lakes.

Mass coral spawning represents a nutrient input to coral reef systems that for Pacific reefs has been shown to stimulate pelagic and benthic processes. If phytoplankton in the water column over the reef are able to utilize this annual nutrient input, this could potentially alter phytoplankton biomass and community composition, in what is normally a very oligotrophic system. Sampling was performed at East Flower Garden Bank (EFGB), Gulf of Mexico during May, July, and August 2009. The annual coral spawning event occurred there August 11-14, 2009. Samples were collected morning and evening at three depths and analyzed for nutrients, chlorophyll a, accessory pigments, phytoplankton species composition, and carbon, hydrogen, and nitrogen (CHN). During spawning, only small changes in nutrient concentrations were detected. Dissolved inorganic nitrogen (DIN) peaked on the second day of spawning and N:P ratio was highest on 5/28, likely due to particularly phosphate concentration. Chl a biomass was significantly different between sample dates and the biomass increased steadily throughout the spawning period. The contribution of different phytoplankton classes to total chlorophyll a was determined using known pigment algorithms. Prokaryotes were the dominant class across the entire sampling period with 60-80 percent abundance. Trichodesmium spp. was the dominant genus throughout the study and genus specific changes per sample date were seen. On 8/11 and 8/13 two genera contributed the majority of chl a (Trichodesmium spp. and Ceratium spp.; Cylindrotheca spp. and Trichodesmium spp., respectively). Abundance showed variability during spawning with a peak at 11 cells/ml on 8/12. The high abundance of Trichodesmium spp. could indicate N limitation is alleviated at the Flower Garden Banks (FGB). Current literature on coral spawning is limited to studies performed in the Great Barrier Reef, with assessment areas close to a major shoreline. Genera found at EFGB were similar to those found in other reef systems. It cannot be determined if nutrient input increased diversity, as diversity was high prior to spawning as well. Greater increase in available forms of nitrogen would have likely been found several days post major spawning. The FGB were a unique system to study, as they are coral reefs, but are located 200 km offshore. This study provided a snapshot into phytoplankton dynamics as a result of spawning. Changes across the short time scale were seen in biomass and community composition.

Satellite images of chlorophyll concentration in the surface waters of the Gulf of Mexico suggest a high degree of heterogeneity in the phytoplankton biomass. The causes of this variability and the amount of variability in the phytoplankton community structure are not well understood. The physical and chemical conditions of a specific environment can influence phytoplankton community structure by selecting for those phytoplankton species able to survive within that environment. Varying salinity and temperature characteristics give water masses distinct surface water density signatures. This study examined the relationship between phytoplankton biomass, community structure, and different water mass properties by measuring chlorophyll a and algal group concentration across frontal zones. Continuous salinity and temperature measurements were used to calculate continuous density along transects during four cruises on the R/V Gyre between summer 2002 and spring 2004. Frontal zones were identified as areas of sharp density change where σt changed by 1.5 points over a distance of 1 km. Density fronts that coincided with visible temperature fronts (satellite AVHRR images) were selected for biomass and community structure analysis. Discrete water samples were analyzed using fluorometric analysis (total chlorophyll a concentration) and HPLC analysis (photosynthetic pigments). Community composition for discrete samples was determined using CHEMTAX and these values were used to interpolate community composition. Phytoplankton biomass and community structure were examined at a total of 21 density fronts. Unlike previous studies of frontal zones, phytoplankton biomass (measured as chl a concentration) was not significantly higher within frontal zones than in adjacent waters at any of the 21 fronts. Community composition (measured as algal group abundance and diversity) was significantly different between the front and at least one adjacent water mass at front 2 during summer 2002, at front 6 during summer 2003, at front 3 during fall 2003, and at front 3 during spring 2004. Both biomass and community composition were significantly different between fronts at all front pairs during summer 2002. The results of this study suggest that density fronts are not biologically important features in the northern Gulf of Mexico. Lack of high phytoplankton biomass at fronts in the Gulf of Mexico could indicate that unique physical, chemical, or biological processes are occurring.

Plankton communities are important members of the food web in coastal systems and are regulated by top-down and bottom-up controls. This study examined the influence of bottom-up controls, such as physicochemical factors, and top-down controls, such as predation, on the plankton communities in South Bay, Texas. Microalgal photopigments were ascertained by high-performance liquid chromatography (HPLC) to determine the relative abundances of major algal classes. Zooplankters were identified to the lowest possible taxon and enumerated. No spatial trends were observed for the physicochemical factors. The northern bay sections exhibited significantly higher phytoplankton and microphytobenthic diatom biomass, probably due to their proximity to the bay inlet. Copepod, gastropod veliger and brachyuran zoea abundances were also higher in this area, albeit insignificantly. The southern bay sections experienced significantly higher cyanobacterial, euglenophyte and chlorophyte biomass, and polychaete larval abundances. Total zooplankton and nauplii abundances were also higher in the southern areas, albeit insignificantly. Sampling the inaccessible areas of the bay in the future may reveal spatial variability among the physicochemical factors which could be influencing the distribution of plankton. Temporal variation for the physicochemical factors followed a typical trend for subtropical climates and influenced the seasonality of the plankton communities. Phytoplankton biomass peaked in February, August and October but these maximums were not significantly different from the other months sampled. Microphytobenthic biomass peaked during the summer months, while diatom biomass also peaked in February. Zooplankton abundances peaked in October, while nauplii and polychaete larvae also peaked in February. Relationships between wind speed, turbidity and the microalgal pigments were assessed to determine if wind-induced resuspension influenced the location of the major algal classes within the water column compared to the sediments. Wind speed and turbidity were directly related to each other, albeit insignificantly. Some phytoplankton and microphytobenthos were considered tychopelagic because wind-induced resuspension increased their biomass in the water column compared to the sediments. The physicochemical factors exerted bottom-up control of plankton community dynamics in this study, while top-down controls, such as predation, require further investigation. Future studies should focus on which of these controls have more influence on plankton community dynamics in South Bay.

This study focused on the spatial and temporal variation of water qualities and the phytoplankton and zooplankton standing crop of southwestern Taiwan. Data were collected from 30 cruises of research and fishermen vessels between August 1999 and December 2002. The yield of sergestid shrimp in this area was also analyzed for possible relationship with physical environment. Concentrations of phosphate and ammonia in the study area were highest in autumn, while the concentration of dissolved silicon peaked in winter; In spring, the concentration of all three nutrients similarly dropped to their lowest level. Regardless of seasonal variation, the concentration of dissolved silicon positively correlated with the water depth around the year. Positive correlation was found between phosphate and river discharge rates of Kao-Ping River in autumn. Negative correlation was found in spring, summer and autumn for ammonia, negative correlation in spring and autumn for dissolved silicon, respectively. Variations of phytoplankton in seasons and locations were also studied. Chlorophyll a blooms in winter but was at lowest level in summer. Concentration of total-chlorophyll a decreased with distance from the shoreline. Concentration of total-chlorophyll a negatively correlated with the river discharge rate in summer, but was positively correlated in winter. Nanophytoplankton (< 10 £gm) appeared to be the major component of phytoplankton. High standing crop of copepods was found at regions near the Liu-Chiu Yu, the estuary of Kao-Ping River, and near the coast. Significant positive correlation between phytoplankton and Copepoda standing crop indicates possible feeding and grazing relationship between them. Changes of the concentration of chlorophyll a corresponds with relative levels of phosphate and ammonia in some seasons. The period with the highest phytoplanktonic standing crop also coincides with the high yield season of sergestid shrimp (from November to March). Variation of Copepoda standing crop does not follow seasonal changes of phytoplankton. Based on the results from General Linear Model analysis, lunar phases, river discharge rates of Kao-Ping River, and the Fishing Effort (hrs/haul-boat) have significant effects on the yield (kgs/haul-boat) of sergestid shrimp respectively. The yield of sergestid shrimp in the full moon period and the last quarter of a lunar month is higher than other periods. The occurrence of thick thermocline water layer matched with the period of high CPUE of sergestid shrimp.

Thesis (M.S.) in Oceanography--University of Maine, 2002. / Includes vita. Bibliography: leaves 72-78.

The research presented here deals with inferring phylogenetic trees and their use to study the evolution of functional traits in diatoms (Heterokontophyta: Bacillariophyceae). Two chapters are concerned with the phylogeny of a mainly freshwater group, the Cymbellales, with a convoluted taxonomic history and classification. I generated a multi-gene dataset to test the monophyly of the Cymbellales and reconstruct the relationships within the order. The molecular data were equivocal with respect to the monophyly of the Cymbellales, especially when taking into account some problematic taxa like Cocconeis and Rhoicosphenia. Aside from the problem with their monophyly, my work shows that the current genus- and family-level classification of the Cymbellales is unnatural, arguing for the need of nearly wholesale re-classification of the group. The two following chapters make use of phylogenetic trees to model the evolution of functional traits. I explored the evolution of cell size across the salinity gradient finding that the opposing selective forces exerted by marine and fresh waters select for different optimal cell sizes -- larger in the oceans and smaller in lakes and rivers. Thereafter, I modelled the evolutionary histories of habitat preference (planktonic-benthic) and growth form (solitary-colonial) across the diatoms. These traits exhibit markedly different evolutionary histories. Habitat preference evolves slowly, is conserved at the level of large clades, and its evolution is generally uniform across the tree. Growth form, on the other hand, has a more dynamic evolutionary history with frequent transitions between the solitary and colonial growth forms and rates of evolution that vary through time. I hope that these empirical studies represent an incremental advancement to the understanding of the evolution diatom species and functional diversity. / text

Since the 'discovery' of the high abundance of viruses in aquatic environments, it has been generally assumed that viruses in lakes are similar to those in oceans. I directly compared these two systems using a large, robust data set. Viral abundance was significantly different among the surveyed environments. The relationship between viral and bacterial abundance indicated a fundamental difference between lakes and oceans, and suggested that viruses infecting phytoplankton may be more important in lakes. Molecular techniques (PCR & DGGE) were used to document spatial and temporal variations in the richness of viruses that infect eukaryotic phytoplankton (Phycodnaviridae) in lakes at the Experimental Lakes Area (ELA). Phycodnavirus richness was highest in the eutrophic lake, and during the spring/early summer in all the lakes. Viral richness was closely associated with phytoplankton abundance and composition. As a result, richness was influenced by trophic status, while patterns of richness were affected by regional climatic conditions. Phylogenetic analysis of environmental Phycodnavirus DNA polymerase (pol) sequences indicated that freshwater Phycodnaviruses are genetically different from cultured isolates and marine environmental sequences. A genetic distance analysis indicated that pol sequences > 7 % different infected different host species. Therefore, the 20 different freshwater sequences likely infected nine different hosts. Multivariate statistics identified seven possible phytoplankton hosts, including chlorophytes, chrysophytes, diatoms and dinoflagellates. Finally, the modified dilution experiment was evaluated as an approach for estimating viral-mediated phytoplankton mortality in two lakes at the ELA. Experiments resulted in non-significant apparent growth rate regressions. While a model analysis, indicated that the method was sensitive to poorly constrained parameters such as burst size and length of the lytic cycle, making it unsuitable for estimating mortality rates in these lakes. These studies indicate that Phycodnaviridae are a genetically rich and dynamic component of lakes. Their richness is influenced by both the chemical and physical components of their environment. Although the presence of these viruses indicates that they are a source of phytoplankton mortality, the magnitude of their impact on structuring phytoplankton communities awaits methodological advances. Nonetheless, these findings support the view that viruses infecting phytoplankton are ecologically important components of lake ecosystems.

No description available.

Interspecific competition for phosphorus was studied in continuous lake water cultures of phytoplankton communities from Lake Memphremagog, and its outcome shown to depend primarily on the size of the competitors. Comparison of the activity of the inducible enzyme alkaline phosphatase between the lake and cultures operated at various dilution rates (0.06-0.93 day('-1)) established that in situ phosphorus limitation rarely depressed average community growth rates much below 0.6 day('-1). Growth rates under comparable, or even more severe (0.2-0.3 day('-1)) limitation in culture decreased significantly with increasing cell size. Phosphorus uptake kinetics and cell quotas measured by track autoradiography on the competing species showed that biomass and quota-specific uptake rates varied inversely with cell size in highly significant allometric regressions, correctly predicting that competitive outcome should depend on size. Substituted in a variable internal stores model of phosphorus limited growth, the allometric functions for uptake, cell quota, and maximum growth rate correctly predicted observed growth rates except in competition much stronger than natural (D = 0.06-0.2 day('-1)). Failure of the model to encompass cell senescence and death due to severe nutrient stress appeared to account for the discrepancy. Neither the absolute difference in growth rates due to cell size nor the direction of selection among the naturally co-occurring species varied systematically with the intensity of competition, indicating that spatio-temporal variation of limiting phosphorus supply in nature is unlikely to directly select the size or taxonomic composition of phytoplankton communities.