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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

The effects of pathogens on club cell investment in fathead minnows, Pimephales promelas

Pollock, Robyn Jennifer 16 March 2011
Fish skin is a metabolically active tissue that responds quickly to stressors and is the first line of defence against physical damage. Club cells, characteristic components of Ostariophysian fish skin, release their contents into the surrounding water upon rupture (e.g. during predation). These chemical cues act as public information of predation risk. Despite the assumption that club cells evolved under the selective force of predation, studies demonstrated that predation has no effect on club cell investment. Rather, club cell production is stimulated by skin penetrating pathogens and parasites. The experiments in this thesis investigate the responses of fish skin to manipulated pathogen risk. In the first experiment, fathead minnows (Pimephales promelas) were exposed to varying infective risk from two pathogen species that differ in pathogenicity, Saprolegnia ferax and S. parasitica. Although there was no difference in club cell density between fish exposed to the two Saprolegnia species, fish exposed to high concentrations of the pathogens had smaller club cells than those exposed to low concentrations. These results are the first to demonstrate a pathogen effect on the size of club cells. The second experiment investigated whether the physical presence of the pathogen was necessary for an alteration in epidermal parameters or whether Saprolegnia parastitica conditioned water was the only stimulus necessary to evoke a change. Results indicated a lack of treatment effect on club cell density, club cell size or epidermal thickness. The third experiment investigated the timing of club cell changes following a pathogen challenge. Although fish exposed to the Saprolegnia ferax treatment had higher club cell density than fish exposed to the control, there was no difference in club cell density between fish sacrificed on day 3, 6, 9 or 12. A portion of the test population for the third experiment was infected with black spot disease. When analyzed separately, trematode infected fish had smaller club cells than those that were uninfected. In light of inconsistent epidermal responses to pathogen challenges, and comparison with other studies, assessment of environmental stressors and population differences that may affect experimental outcomes and potentially interact with infectious agents is advised.
2

The effects of pathogens on club cell investment in fathead minnows, Pimephales promelas

Pollock, Robyn Jennifer 16 March 2011 (has links)
Fish skin is a metabolically active tissue that responds quickly to stressors and is the first line of defence against physical damage. Club cells, characteristic components of Ostariophysian fish skin, release their contents into the surrounding water upon rupture (e.g. during predation). These chemical cues act as public information of predation risk. Despite the assumption that club cells evolved under the selective force of predation, studies demonstrated that predation has no effect on club cell investment. Rather, club cell production is stimulated by skin penetrating pathogens and parasites. The experiments in this thesis investigate the responses of fish skin to manipulated pathogen risk. In the first experiment, fathead minnows (Pimephales promelas) were exposed to varying infective risk from two pathogen species that differ in pathogenicity, Saprolegnia ferax and S. parasitica. Although there was no difference in club cell density between fish exposed to the two Saprolegnia species, fish exposed to high concentrations of the pathogens had smaller club cells than those exposed to low concentrations. These results are the first to demonstrate a pathogen effect on the size of club cells. The second experiment investigated whether the physical presence of the pathogen was necessary for an alteration in epidermal parameters or whether Saprolegnia parastitica conditioned water was the only stimulus necessary to evoke a change. Results indicated a lack of treatment effect on club cell density, club cell size or epidermal thickness. The third experiment investigated the timing of club cell changes following a pathogen challenge. Although fish exposed to the Saprolegnia ferax treatment had higher club cell density than fish exposed to the control, there was no difference in club cell density between fish sacrificed on day 3, 6, 9 or 12. A portion of the test population for the third experiment was infected with black spot disease. When analyzed separately, trematode infected fish had smaller club cells than those that were uninfected. In light of inconsistent epidermal responses to pathogen challenges, and comparison with other studies, assessment of environmental stressors and population differences that may affect experimental outcomes and potentially interact with infectious agents is advised.
3

Aspects of Fathead Minnow Reproductive Behavior

Pyron, Mark 05 1900 (has links)
Following a study of normal reproductive behavior of fathead minnows, Pimephales promelas, experiments were conducted to determine the stability/variability of behavior by sexually mature, territorial males under a variety of manipulated conditions. Collectively, these experiments indicate that although the individual behaviors of fathead minnows appear to be quite variable, the overall process, reproductive behavior, is stable.
4

Effects of Suspended Solids on Bioavailability of Chemicals to Daphnia magna and Pimephales promelas

Hall, W. Scott (Warren Scott) 12 1900 (has links)
Three suspended solids types containing a range of physicochemical characteristics were used to determine the effect of suspended solids on the bioavailability of acenaphthene, 1,2,4,5-tetrachlorobenzene, zinc, and chlordane to Daphnia magna and Pimephales promelas. Generally, the bioavailability of zinc and chlordane decreased due to interactions with all suspended solids types while bioavailability of acenaphthene and 1,2,4,5-tetrachlorobenzene were not clearly reduced. Partition coefficients and slope of dose-response curves related chemical characteristics and organism sensitivity, respectively, to experimentally determined results. It is believed that the biologically available form of these chemicals to Daphnia magna and Pimephales promelas resides in the aqueous phase.
5

Predation Cues Influence Metabolic Rate and Sensitivity to Other Chemical Stressors in Fathead Minnows (Pimephales promelas) and Daphnia pulex

Robinson, Amie L., Chapman, Trevor, Bidwell, Joseph R. 03 November 2017 (has links)
The response of aquatic species to contaminants is often context dependent as illustrated by the influence that predation cues can have on the toxicity of some chemicals. We sought to gain additional insight into this interaction by examining how predation cues (alarm cue and fish kairomone) influence metabolic rate and the acute toxicity of sodium chloride and cadmium to fathead minnow larvae (Pimephales promelas) and sodium chloride to Daphnia pulex neonates. Consistent with a “flight or fight” response, the metabolic rate of fish larvae was elevated in the presence of alarm cue and growth of the minnows was also significantly reduced when exposed to alarm cue. The average 48-h LC50 for fathead minnows exposed to sodium chloride was significantly lower in the presence of alarm cue and kairomone combined as compared to tests with the salt alone. Analysis of the dose and survival response indicated alarm cue increased sensitivity of the fish to mid-range salt concentrations in particular. These results suggest an energetic cost of exposure to predation cues that resulted in enhanced toxicity of NaCl. Exposure to kairomone alone had no significant effect on salt toxicity to the minnows, which could be related to a lack of previous exposure to that cue. The acute toxicity of cadmium to the fish larvae was also not affected by the presence of predation cues which could be due to a metal-induced sensory system dysfunction or reduced bioavailability of the metal due to organic exudates from the predation cues. In contrast to the fathead minnow results, the metabolic rate of D. pulex and toxicity of NaCl to the daphnids were reduced in the presence of certain predator kairomones. This suggests an anti-predator response that enhanced tolerance to the salt. This study illustrates that the effect of predation cues on toxicity of aquatic contaminants can vary significantly based on the prey species, type of cue, and chemical stressor.
6

An Evaluation of the Short-Term Embryo-Larval and Seven-Day Larval Test Methods for Estimating Chronic Toxicity of Zinc to the Fathead Minnow (Pimephales promelas)

Stewart, Susan Michels 05 1900 (has links)
Chronic toxicity of zinc to Pimephales promelas was estimated by conducting replicate static and static-renewal short-term embryo-larval tests and static-renewal seven-day larval tests. The two test methods were highly reproducible. Daily renewal of test solutions had little effect on the toxicity of zinc, however, the stage of development at which exposure was initiated affected the sensitivity of the toxic endpoints measured. The most sensitive and reproducible endpoint in the embryo-larval tests was survival of viable (non-deformed) larvae and in the seven-day larval test was growth of the larvae, which was slightly more sensitive than the embryo-larval test endpoint. The estimated MATC of 0.18 and 0.15 mg/L mean total and mean soluble zinc, respectively, compared well with published results. Because of its advantages and similar sensitivity, the short-term embryo-larval test was recommended for estimating chronic toxicity.
7

Assessment of the Efficacy of a Constructed Wetland to Reduce or Remove Wastewater Effluent Estrogenicity and Toxicity Using Biomarkers in Male Fathead Minnows (Pimephales Promelas Rafinesque, 1820)

Hemming, Jon M. 12 1900 (has links)
Vitellogenin in Pimephales promelas was used to assess estrogenicity of a local municipal effluent. Vitellogenin induction in male P. promelas increased in frequency and magnitude with increased exposure duration and was greater ("=0.05) than controls after 2 and 3 weeks of exposure. The level of vitellogenesis induced by effluent exposure was high compared to similar studies. A spring season evaluation followed. Biomarkers in P. promelas were used to assess the efficacy of a treatment wetland to remove toxicity and estrogenicity in final treated wastewater effluent. Comparisons were made with an effluent dominated stream and laboratory controls. Vitellogenin, GSIs (gonado-somatic indices), HSIs (hepato-somatic indices) and secondary sexual characteristics were biomarkers used in P. promelas models to assess aqueous estrogenicity. Biological indicators used to assess general fish health included hematocrit and condition factors. The estrogenic nature of the effluent was screened, concurrent with fish exposure, with GC/MS analysis for target estrogenic compounds including: 17-b estradiol, estrone, ethynylestradiol, Bisphenol A, nonylphenolic compounds, phthalates, and DDT. Plasma vitellogenin measured in P. promelas was significantly elevated (p < 0.0001) at the inflow site of the wetland and stream sites. GSIs for these exposures were less (a=0.001) at the wetland inflow site. At wetland sites closest to the inflow, secondary sexual charateristics, tubercle numbers and fat pad thickness, were less (a=0.0001). Hematocrit and condition factors were less (a=0.001) at sites closer to the wetland inflow. Seasonal variation was examined by repeating the effluent characterization in summer. Additionally, summer testing included exposure to an effluent dilution series. Fish condition heavily influenced interpretation of the results. Pre-acclimation exposure to spawning stresses may have altered many of the biological markers measured. Results are discussed relative to fish health and pre-exposure environment. Toxicity assessed with P. promelas biomarkers was compared with Ceriodaphnia dubia and Vibrio fischeri toxicty tests on this effluent. Biomarkers of fish health were somewhat less sensitive than C. dubia test endpoints, but more sensitive than V. fisheri.
8

Threat-sensitive learning and generalization of predator recognition by aquatic vertebrates

Ferrari, Maud C.O. 29 January 2009
Many prey species lack innate recognition of their potential predators. Hence, learning is required for them to recognize and respond to predation threats. When wild-caught, these same species may show amazing sophistication in their responses to predator cues. They are able to adjust the intensity of their antipredator responses to a particular predator according to the degree of threat posed by that predator. This ability is therefore acquired through learning. While many studies have shown that prey can learn to respond to predator cues through different learning modes, little is known about what the prey are actually learning. The results presented in this thesis show that learned predator recognition goes beyond the simple labelling of predators as dangerous. Using fathead minnows (Pimephales promelas), woodfrog (Rana sylvatica) tadpoles and boreal chorus frog (Pseudacris maculata) tadpoles, I demonstrated that a one time learning event, either through pairing with alarm cues or through social learning, was enough for prey to learn the level of threat associated with the novel predator cues. I showed that the level of danger associated with the predator cues was determined by the concentration of alarm cues when learning through pairing of alarm cues, or by the intensity of antipredator response displayed by the tutors and by the tutor-to-observer ratio when learning occurred through cultural transmission. Moreover, when subsequently exposed to predator cues, prey adjusted their antipredator responses according to the change in concentration of predator cues between the learning event and the subsequent exposure. Prey displayed stronger antipredator responses when exposed to higher concentrations of predator cues and vice versa. When minnows were provided with conflicting information about the danger level associated with a predator, they displayed a safety strategy and used the most recent information available to respond to predation threats. On a longer time scale, the data also suggest that woodfrog tadpoles are able to learn to respond to predation threats according to the risk posed by the predator at different times of day. Finally, I showed that prey learn to recognize particular characteristics of predators and can generalize their antipredator responses to novel species sharing those characteristics. However, generalization of predator recognition is dependent on the level of risk associated with the predator. Threat-sensitive learning is an extremely complex process shaped by the millions of years of selection imposed by predators on prey.
9

Threat-sensitive learning and generalization of predator recognition by aquatic vertebrates

Ferrari, Maud C.O. 29 January 2009 (has links)
Many prey species lack innate recognition of their potential predators. Hence, learning is required for them to recognize and respond to predation threats. When wild-caught, these same species may show amazing sophistication in their responses to predator cues. They are able to adjust the intensity of their antipredator responses to a particular predator according to the degree of threat posed by that predator. This ability is therefore acquired through learning. While many studies have shown that prey can learn to respond to predator cues through different learning modes, little is known about what the prey are actually learning. The results presented in this thesis show that learned predator recognition goes beyond the simple labelling of predators as dangerous. Using fathead minnows (Pimephales promelas), woodfrog (Rana sylvatica) tadpoles and boreal chorus frog (Pseudacris maculata) tadpoles, I demonstrated that a one time learning event, either through pairing with alarm cues or through social learning, was enough for prey to learn the level of threat associated with the novel predator cues. I showed that the level of danger associated with the predator cues was determined by the concentration of alarm cues when learning through pairing of alarm cues, or by the intensity of antipredator response displayed by the tutors and by the tutor-to-observer ratio when learning occurred through cultural transmission. Moreover, when subsequently exposed to predator cues, prey adjusted their antipredator responses according to the change in concentration of predator cues between the learning event and the subsequent exposure. Prey displayed stronger antipredator responses when exposed to higher concentrations of predator cues and vice versa. When minnows were provided with conflicting information about the danger level associated with a predator, they displayed a safety strategy and used the most recent information available to respond to predation threats. On a longer time scale, the data also suggest that woodfrog tadpoles are able to learn to respond to predation threats according to the risk posed by the predator at different times of day. Finally, I showed that prey learn to recognize particular characteristics of predators and can generalize their antipredator responses to novel species sharing those characteristics. However, generalization of predator recognition is dependent on the level of risk associated with the predator. Threat-sensitive learning is an extremely complex process shaped by the millions of years of selection imposed by predators on prey.

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