Spelling suggestions: "subject:"pituitary"" "subject:"poituitary""
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Desensitisation of the pituitary vasopressin receptor : development of a model system to assess involvement of G protein-coupled receptor kinase 5.Gatehouse, Michelle January 2008 (has links)
The hypothalamic peptide arginine vasopressin (AVP) is an important regulator of adrenocorticotropin (ACTH) release from the anterior pituitary. AVP stimulates ACTH secretion from corticotroph cells by activating the pituitary vasopressin receptor (V1b-R), a member of the G protein-coupled receptor (GPCR) family. In vitro, repeated stimulus of anterior pituitary cells with AVP results in rapid desensitisation. The aim of this research was to develop methods needed to use RNA interference (RNAi) to investigate the role of G protein-coupled receptor kinase 5 (GRK5) in this desensitisation process. This required the development of a model system using human embryonic kidney (HEK) 293 cells transfected with the pituitary vasopressin receptor, V1b-R. AVP binding to the V1bR activates the phosphoinositide signalling pathway, leading to production of inositol phosphates (IPs), which can be measured following radiolabelling of cells with myo-[³H]inositol. Stimulation of V1b-R-transfected cells for 15 min with AVP (100nM) increased IP production to 235.5 ± 23.4 % (n=3, p<0.02) of that seen in un-stimulated control cells. Following a 5 minute pre-treatment with 5nM VP, the IP response to stimulation with 100nM VP for 15 min was reduced to 62.8 ± 9.1 % (n=4, p<0.02) of that seen in control cells that were not pre-treated. These data indicate that AVP-desensitisation can be induced and measured in V1bR-transfected HEK293 cells following a brief pre-treatment with a physiological concentration of AVP. This model system will enable RNAi to be used to investigate the role of GRK5 in AVP-desensitisation. When using RNAi, it is essential to establish that the effects observed are the result of small interfering RNA (siRNA) specific degradation of the target mRNA. Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of GRK5 at the mRNA level in HEK293 cells. Human GRK5 mRNA was amplified using qRT-PCR with GRK5 specific primers, providing confirmation that GRK5 is expressed endogenously in HEK293 cells. GRK5 expression studies were carried out to evaluate whether the qRT-PCR methods developed would be suitable to measure knockdown of GRK5 mRNA using RNAi. These experiments were also designed to assess the impact of HEK293 cell culture methods on expression of GRK5. Expression of GRK5 did not vary with passage number (2-26 passages). The GRK5 expression in HEK293 cells that were maintained in culture for 5 days (grown to a confluence of approximately 100%) was 7.4 ± 0.9 fold greater (n=2, p<0.05) than for cells cultured for 3 days (grown to a confluence of approximately 65%). These data indicate that GRK5 expression is affected by HEK293 culture conditions. Furthermore, the results demonstrated that a significant difference in GRK5 expression could be measured in HEK293 cells using qRT-PCR. Therefore the results reported in this thesis provide the basis for future studies utilising RNAi to investigate mechanisms underlying V1b-R desensitisation.
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Circadian rhythms in the neuroendocrine dopaminergic neurons regulating prolactin secretionSellix, Michael Timothy. Freeman, Marc E. January 2005 (has links)
Thesis (Ph. D.)--Florida State University, 2005. / Advisor: Dr. Marc E. Freeman, Florida State University, Program in Neuroscience. Title and description from dissertation home page (viewed June 8, 2005). Document formatted into pages; contains xi, 172 pages. Includes bibliographical references.
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Effects of prenatal stress on lever-press acquisition, delay discounting, and ethanol self-administration in ratsBruner, Natalie R. January 2010 (has links)
Thesis (Ph. D.)--West Virginia University, 2010. / Title from document title page. Document formatted into pages; contains vi, 67 p. : ill. Includes abstract. Includes bibliographical references (p. 59-67).
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Separation and purification of follicle stimulating and luteinizing hormones from pituitary glandsLeonora, John, January 1957 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1957. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 214-215).
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Studies on anterior pituitary gonadotropin content of Coturnix quail and turkeysBacon, Wayne Lee, January 1965 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 45-50.
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Hypothalamic-pituitary function following cranial irradiation for nasopharyngeal carcinoma /Lam, Siu-ling, Karen. January 1900 (has links)
Thesis (M.D.)--University of Hong Kong, 1990.
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Biphasic growth hormone release induced by protein kinase C activation in grass carp pituitary cells /Chu, Mei-sze. January 1999 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 76-108).
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Aparato sexual femenino del Bufo arenarum variaciones cíclicas y mecanismos reguladores de su actividad y estructura,López Colombo de Allende, Inés. January 1938 (has links)
Tesis--Córdoba (Argentina). / "General conclusions" in English: p. 156-158. "Bibliografía": p. 159-164.
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The synthesis of cystinol hydrochloride ...Kroll, Harry, January 1946 (has links)
Thesis (Ph. D.)--University of Chicago, 1943. / Includes bibliographical references.
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Effects of steroids and releasing hormones on LH production in cultures of adult turkey pituitary cellsBirrenkott, Glenn, January 1978 (has links)
Thesis--Wisconsin. / Vita. Includes bibliographical references (leaves 44-48).
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