Thesis (Ph. D.)--University of Washington. / Bibliography: l. 72-75.

Thesis (M.S.)--University of Wisconsin--Madison, 1983. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 68-76).

Thesis (M.S.)--California Polytechnic State University, 2009. / Mode of access: Internet. Title from PDF title page; viewed on August 6, 2009. Major professor: Dr. Lauren Garner. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Agriculture with specialization in Crop Science." "June 2009." Includes bibliographical references (p. 37-49).

Thesis (M.S.) -- Worcester Polytechnic Institute. / Keywords: Phytohormones. Includes bibliographical references (p.73-79).

The objectives of this thesis were to trace the developmental pathways of somatic embryos of an abscisic-acid independent line of Larix x eurolepis. to catalogue the phenotypes of mature embryos, to determine critical stages of development and to attempt to increase the number of maturing somatic embryos. The low rate of maturation could not be entirely explained by differences in phenotypes of early embryos, critical stages of development, or the lack of plant growth regulators in the medium. In addition, the shape and epidermal type of the mature embryo did not always determine the type of epicotyl produced, nor did it affect the rooting and mortality rates. Six types of embryonal structures were evident in the aggregates of line 2086: (1) a smooth (SEMLS) or (2) rough (REMLS) embryonal mass subtended by a cylindrical, compact, long suspensor. (3) a rough embryonal mass subtended by a long, loose suspensor (REMLLS). (4) a rough embryonal mass subtended by a suspensor arising from greater than one quarter of the surface area of the embryonal mass (REMST). (5) a rough embryonal mass subtended by a short, compact, cylindrical suspensor (REMSS). and (6) a cluster of meristematic cells which may or may not have single suspensor cells attached (MC). For isolated embryonal structures of all types, to continue development into a nodule or a mature embryo was the least common fate, while proliferation and developmental arrest were more common. In general, the more organized embryonal structure types (SEMLS and REMLS) had higher rates of maturation compared to the other 4 types but the most common fate was still developmental arrest (74% SEMLS. 62% REMLS), followed by proliferation (10% SEMLS. 30% REMLS), and nodule or embryo development (16% SEMLS. 9% REMLS). REMLLS and REMST embryonal structures became developmentally arrested or proliferated (43-47%) while the rate of nodules/mature embryos production was 9-11%. Neither individual REMSS nor MC structures produced any nodules or mature embryos, but REMSS had a lower rate of developmental arrest (81%) and a higher rate of proliferation (19%) than MC (89% and 11% respectively). Embryos at more advanced stages of development were less likely to die, become developmentally arrested or become nodules, but more likely to become mature embryos than embryos at less advanced stages of development. A critical stage of development appeared to be the focal zone stage at the formation of a complete polyphenol band around the basal end of the embryonal mass. At this stage, the majority of immature embryos became mature embryos (61%) while only 3% of the embryos died. 10% became developmentally arrested, and 20% became nodules. The majority of mature somatic embryos were normally proportioned with a smooth epidermis (43%) rather than vitrified (12%). normal with a rough epidermis (12%) or misshapen (smooth or rough. 33%). The shape of the mature embryo was associated with the type of epidermis, with mature somatic embryos with normal proportions more likely to have smooth epidermis (78%) than a rough epidermis (22%) while mature embryos with abnormal proportions were as likely to have a smooth epidermis as a rough epidermis. The shape of the mature embryo was associated with the shape of the epicotyl produced. Normal-smooth, mature embryos were more likely to produce normal-smooth epicotyls (73%) than twin epicotyls (21%), vitrified epicotyls (2%) or misshapen epicotyls (5%) compared to vitrified mature embryos (42% normal-smooth epicotyls, 34% twin epicotyls, 23% vitrified epicotyls, 1% misshapen epicotyls) or misshapen mature embryos (22% normal-smooth epicotyls, 47% twin epicotyls, 7% vitrified epicotyls, 24% misshapen smooth/rough embryos). The number of mature embryos which germinated or died was not associated with either the epidermal quality or the shape of the mature embryo. Few SEMLS or REMLS embryonal structures responded to auxin and cytokinin treatments. There appeared to be a trend towards less developmental arrest and proliferation and more nodules/mature embryos produced on media with no auxin compared to media with 2,4-D and a trend towards more developmental arrest and fewer nodules/mature embryos on media without BA compared to media with BA. Only nodules on media without plant growth regulators produced roots or cotyledons. There was no effect of embryonal structure type (SEMLS or REMLS), or sucrose concentration (58 μM or 174 μM) on the maturation of immature embryos, but on media without ABA, fewer immature embryos proliferated or became developmently arrested and more embryos became nodules or mature embryos than on medium with 6-24 μM ABA. / Graduate

Physiologic responses to Gibberellic Acid (GA), I-Naphthalene Acetic Acid (NAA), Benzylaminopurine (BAP), and Abscisic Acid (ABA). suggest that Oosterium monilfferum has the ability to utilize these plant growth factors. The growth promoters NAA and GA both increased growth when added to the media. The cell division regulator BAP (a synthetic cytokinin). also had a promotive effect on growth. Abscisic acid was found to be inhibitive to growth.

No description available.

Exogenous application of BA (0, 50, 100 mg ul--1) had no significant effects on tolerance of chili peppers to salt (--0.75 MPa NaCl:CaCl₂, 3:1, w/w) as measured by vegetative and reproductive weights, numbers of reproductive structures, transpiration and total chlorophyll. When peppers were applied with microliter amounts of ¹⁴C labelled benzyladenine (BA; 44,400 dpm 1⁻¹), BA was translocated primarily acropetally from the site of application. Regardless of application site, translocated BA was ported primarily to expanding leaves, and BA was more readily absorbed by leaf as opposed to stem surfaces. Exogenous application of BA (0, 50, 100 mg ul⁻¹) had no significant effects on tolerance of chili peppers to salt (-0.75 MPa NaCl:CaCl₂, 3:1, w/w) as measured by vegetative and reproductive weights, numbers of reproductive structures, transpiration and total chlorophyll.

by Bi Yu Rong. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 188-220). / Acknowledgment --- p.i / Abstract --- p.ii / Table and contents --- p.v / List of abbreviation --- p.x / List of Tables --- p.xi / List of Figures --- p.xiv / Chapter Chapter 1 --- Introduction --- p.1 / Chapter Chapter 2 --- Literature review --- p.5 / Chapter 2.1 --- General information of plant growth regulators --- p.5 / Chapter 2.2 --- Natural plant growth inhibitors --- p.14 / Chapter 2.3 --- Alkaloids and narciclasine --- p.15 / Chapter 2.4 --- Studies on expansion and greening of cotyledons --- p.22 / Chapter 2.5 --- Investigation on chlorophyll synthesis --- p.28 / Chapter Chapter 3 --- Materials and methods --- p.31 / Chapter 3.1 --- Plant materials --- p.31 / Chapter 3.2 --- Isolation and purification of inhibitory substance from Narcissus bulbs --- p.31 / Chapter I. --- Isolation of inhibitory substance from fresh Narcissus bulbs --- p.31 / Chapter II. --- Partial purification of the inhibitory substance with different organic solvents --- p.32 / Chapter III. --- Purification and identification --- p.32 / Chapter A. --- Thin layer chromatography (TLC) --- p.32 / Chapter B. --- Column chromatography --- p.33 / Chapter C. --- Spectrometric analyses --- p.33 / Chapter IV. --- Bioassays --- p.34 / Chapter 3.3 --- Effect of narciclasine (NCS) on the seeds germination and seedling growth --- p.34 / Chapter I. --- Germination experiments --- p.35 / Chapter II. --- Seedlings growth --- p.35 / Chapter 3.4 --- Interaction of NCS and phytohormones --- p.35 / Chapter I. --- Interaction with abscisic acid (ABA) --- p.36 / Chapter A. --- Seed germination --- p.36 / Chapter B. --- Seedling growth --- p.36 / Chapter II. --- Interaction with auxin --- p.36 / Chapter III. --- Interaction with gibberellin --- p.37 / Chapter VI. --- Interaction with cytokinin --- p.38 / Chapter 3.5 --- Interaction of NCS and phytohormones to growth and greening of excised radish cotyledons exposing to light --- p.39 / Chapter I. --- Growth of excised radish cotyledons exposing to light --- p.39 / Chapter II. --- Chlorophyll content determination --- p.40 / Chapter III. --- Effects of a pretreatment with BA or NCS on the growth and greening of excised radish cotyledons --- p.40 / Chapter 3.6 --- Effect of NCS on the growth and greening of excised radish cotyledons and etiolated wheat leaves --- p.41 / Chapter I. --- Effect of NCS on the growth and greening of excised radish cotyledons --- p.41 / Chapter II. --- Effect of NCS on the greening of etiolated wheat leaves --- p.41 / Chapter 3.7 --- Effect of NCS on chlorophyll synthesis and δ-aminolevulinic acid (ALA) accumulation of etiolated wheat leaves in presence of levulinic acid (LA) --- p.42 / Chapter 3.8 --- Enzymes studies in the excised radish cotyledons --- p.43 / Chapter I. --- Assay of isocitrate lyase activity --- p.44 / Chapter II. --- Assay of hydroxypyruvate reductase activity --- p.44 / Chapter 3.9 --- Ultrastructural studies --- p.45 / Chapter Chapter 4. --- Results --- p.47 / Chapter 4.1 --- Chemical studies of NCS --- p.47 / Chapter I. --- Isolation and partial purification of inhibitory substance from Narcissus bulbs --- p.47 / Chapter A. --- "Effect of lyophilized slimy secretion (LSS) on the germination seeds, the growth of radicle and hypocotyl of seedlings of Brassica" --- p.47 / Chapter B. --- Effect of different solvent extracts on the germination and the elongation of radicle and hypocotyl of Brassica seedlings --- p.47 / Chapter C. --- Effect of fraction isolated with n-butanol from dried bulbs or LSS on the germination of Brassica seeds and radicle growth --- p.49 / Chapter D. --- Purification of inhibitory substance from Narcissus bulbs by chromatography --- p.54 / Chapter II. --- Identification of the inhibitory substance from Narcissus bulbs .… --- p.62 / Chapter 4.2 --- Physiological and biochemical studies ofNCS --- p.70 / Chapter I. --- Effects ofNCS on seed germination and seedlings growth of Brassica --- p.70 / Chapter II. --- Time course studies ofNCS on germination and growth of radish seeds --- p.70 / Chapter III. --- Comparative studies ofNCS and ABA on seeds germination and seedlings growth --- p.73 / Chapter IV. --- Interaction between NCS and phytohormones --- p.79 / Chapter A. --- Interaction of NCS with ABA --- p.79 / Chapter B. --- Interaction of NCS with IAA --- p.84 / Chapter C. --- Interaction of NCS with gibberellin --- p.84 / Chapter D. --- Interaction of NCS with cytokinin --- p.89 / Chapter V. --- Effects ofNCS and BA on chlorophyll and carotenoid content of excised cotyledons --- p.89 / Chapter A. --- "Effects ofNCS and BA on expansion, chlorophyll content and carotenoid content of excised radish cotyledons" --- p.89 / Chapter B. --- Effects of a pretreatment with BA or NCS on the growth and greening of excised radish cotyledons --- p.97 / Chapter VI. --- Interaction between NCS and phytohormones in growth and greening of excised radish cotyledons --- p.105 / Chapter A. --- "Effects of BA,GA3 and ABA on the growth and greening of excised radish cotyledons" --- p.105 / Chapter B. --- Interaction of NCS with phytohormones on growth and greening of excised radish cotyledons --- p.108 / Chapter 4.3 --- Investigation of effects of NCS on chlorophyll synthesis --- p.113 / Chapter I. --- Effect of preincubation in water on growth and greening of excised cotyledons under light --- p.113 / Chapter II. --- Effect of NCS on the growth and greening of etiolated radish excised cotyledons --- p.116 / Chapter III. --- Effect of NCS on the greening of etiolated leaves of 7-day-old wheat seedlings under light --- p.116 / Chapter IV. --- Effect of LA on ALA accumulation in the light --- p.120 / Chapter V. --- Time course study of NCS on ALA accumulation in the presence of LA --- p.122 / Chapter 4.4 --- Effect of NCS on the development of enzymes activities in the excised radish cotyledons --- p.122 / Chapter I. --- Effect of NCS on isocitrate lyase activity of excised radish cotyledons --- p.122 / Chapter II --- Effect of NCS on hydroxypyruvate reductase activity of excised radish cotyledons --- p.125 / Chapter 4.5 --- Effect of NCS on ultrastructural changes of excised radish cotyledons --- p.128 / Chapter I. --- Time course studies --- p.128 / Chapter II. --- "Effect ofNCS, BA and ABA on the ultrastructural change of excised radish cotyledons in the light" --- p.142 / Chapter III. --- Effect of a pretreatment with dark on the inhibition ofNCS on ultrastructural change of excised radish cotyledons in light --- p.150 / Chapter Chapter 5. --- Discussion --- p.160 / Chapter Chapter 6. --- Conclusions --- p.180 / References --- p.188

Typescript. / Digitized by Kansas Correctional Industries