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Thesis (D. Sc.)--Faculty of Science, University of Adelaide, 1990. / Published works [representing] original research conducted during the various phases of [his] academic development--Pref. Includes bibliographical references.

Thesis--University of Florida. / Description based on print version record Typescript. Vita. Includes bibliographical references (leaves 88-95).

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Shen, Jinbo. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 110-119). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

Published works [representing] original research conducted during the various phases of [his] academic development--Pref / Includes references / 1 v. (various pagings) : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (D. Sc.)--Faculty of Science, University of Adelaide, 1990

The thesis begins with an introduction to signal transduction and an analysis of current understanding of plant signal transduction. There are similarities between plants and animals, but also key differences, including lack of protein kinase C and of a cAMP signaling pathway in plants, and presence in plants of calcium dependent protein kinase (CDPK), which has a kinase catalytic domain contiguous with a C-terminal calmodulin-like domain. The next section examines protein kinase activity in the plasma membrane (PM) of zucchini hypocotyls. Zucchini PM contains four or more polypeptides with calcium-requiring protein kinase activity. The enzymes appear to be tightly associated with the PM, and at least three are recognized by monoclonal antibody to soybean soluble CDPK. Total proteins from several different organs of zucchini seedlings contain kinases with molecular weights similar to the hypocotyl PM enzymes. In the third section details of partial purification of the solubilized PM kinases are presented. Kinases which do not crossreact with anti-CDPK monoclonal antibody were resolved by anion exchange from ones which do crossreact. Peptide mapping was used to test the relationship between the kinases. Results of peptide mapping suggest that at least three types of protein kinase are present in zucchini PM, two of which are immunologically similar to CDPK and one of which is not. The last section concerns the potential for testing interactions between PM protein kinases and plasma membrane auxin binding proteins (ABP's) by use of photoaffinity labeling of ABP's. Causes of variable photoaffinity labeling by an azido-IAA are considered. Labeling of both the tomato mutant diageotropica and the parent VFN membranes was inexplicably inconsistent. / Graduation date: 1994

Thesis (Ph. D.)--University of Adelaide, Dept. of Botany, 1991. / Includes bibliographical references (leaves 197-209).

Although dynamic interactions between plant cells and their environment require adhesion between the cell wall (CW) and the plasma membrane (PM), few plant adhesion molecules have been identified. Therefore, the seed coat mucilage secretory cells (MSCs) of Arabidopsis thaliana (which undergo developmentally regulated changes in adhesion) were developed into a novel model system to study PM-CW adhesion. Twenty-seven candidate genes were identified using data from publicly available and seed-specific microarrays. Mutant plants for these genes were screened for defects in adhesion via plasmolysis, and for changes in MSC morphology that may result from defective adhesion (Chapter 1). Two fasciclin-like arabinogalactan proteins were isolated in this screen. One of these, SOS5, was characterized in detail (Chapter 2). sos5 mutants are sensitive to hyperosmotic conditions and show defects in PM-CW adhesion and MSC mucilage structure. Interestingly, these phenotypes may be attributed to defects in adhesion or to defects in cell wall deposition.