In response to a growing concern over the rising rates of extinction of the world's plants and of habitat destruction, studies of Southern African threatened plants were initiated in the 1970's. These studies, which have largely concentrated on Western Cape flora, led to the publication of "Threatened Plants of Southern Africa" by Hall et al., 1980, which attempted to list as many threatened or possibly threatened species as possible. It was however marred by a lack of recent herbarium records and detailed studies from many parts of the region, the Eastern Cape being one of these. In order to extend these detailed studies to gain a clearer picture of the numbers of threatened species in the Eastern Cape and evaluate the conservation status of Eastern Cape vegetation this project was initiated. Initially lists of possibly threatened and endemic taxa of the Eastern Cape were compiled from various sources. These listed taxa were then checked against herbarium records, all available information being filled onto index cards for filing purposes. This paper-based filing system was then transferred into a computer-based data bank to facilitate the efficient storage and retrieval of information. Results from this data bank show that there are 662 variously threatened plant taxa in the Eastern Cape, many of which fall into temporary categories which need to be clarified by investigation in the field. Primarily based on the above results, a table ranking the various vegetation types into an order of priority for investigations about conservation requirement was developed.Subtropical Thicket was found to be the vegetation type in most need of investigation and so an extensive phytosociological survey was carried out in the Valley Bushveld which forms the major portion of Subtropical Thicket in the Eastern Cape. Twelve sites were sampled for floristic and environmental variables along a rainfall gradient of between 300 mm yr⁻¹ and 1 000 mm yr⁻¹ and along a longitudinal gradient from the Buffalo River in the east to the Gamtoos-Kromme complex in the west. Floristic data W(re analysed using multivariate techniques of classification and ordination. A classification by two-way species indicator analysis revealed the Valley Bushveld to consist of two orders of thicket, the Kaffrarian Succulent Thicket containing the two suborders, Inland Succulent Thicket and the Coastal Succulent Thicket and the Kaffrarian Thicket containing Coastal Kaffrarian Thicket and Inland Kaffrarian Thicket. Ordina tion by detrended correspondence analysis also grouped sites according to these vegetation categories in a sequence along one axis, to which the rainfall gradient could be related. Variables such as diversity indices, numbers of endemics, numbers of threatened taxa and structural features were also extracted from the data and these were correlated with environmental variables by multiple regression analysis. Species richness and the percent woody component w\!re positively correlated with rainfall while endemism and percent succulent component were strongly negatively correlated with rainfall. Most of the other relationships were explained by interrelationships with rainfall. Finally the sites were evaluated according to floristic criteria indicative of conservation value. The Coastal Succulent Thicket appeared to have the highest conservation value mainly owing to high endemism, while Inland Kaffrarian Thicket was also important as it supports a high number of species. The thickets with high conservation value are therefore the thickets of coastal areas in the western parts of the Eastern Cape which receive a low rainfall and the thickets which receive a rainfall in excess of 800 mm. / KMBT_363 / Adobe Acrobat 9.54 Paper Capture Plug-in

Understanding why some introduced alien plant species become invasive whilst others fail is a fundamental question in ecology, not least because of the considerable ecological and economic damage caused by invasive plants globally. Identifying factors that drive alien plant invasions can inform efforts to predict the nsk of invasion by an introduced plant species. This would allow prevention of introduction of high risk aliens, as well as targeted management of species already introduced that pose the latest threats to the ecolocal integrity of host ecosystems. However, generalisations among the findings of comparative plant invasion studies have been limited by a lack of control of confounding variables such as propagule pressure, time since introduction and phylogeny, a lack of knowledge of introduced species that failed to establish, and inconsistent use of terminology defining the invasion process. This thesis used Amani Botanical Garden (ABG) in the East Usambara Mountains of Tanzania, as a comparative case study system to assess the relative ability of multiple factors to explain invasion success of species introduced to a tropical forest ecosystem.

Thesis (MTech (Horticulture))--Cape Peninsula University of Technology, 2016. / This study focused on optimising four types of in-vitro conservation methods, namely: 1), micropropagation, 2) in-vitro slow growth, 3) seed germination and 4) cryopreservation for selected endangered plant species indigenous to the Cape Floristic Region. It is one of the targets set by United Nations millennium development goals, to integrate different conservation measures in order to preserve plant diversity and mitigate losses of genetic diversity. Therefore this study uses Phalaenopsis hybrids as a trial species that can be studied for the conservation of endangered Disa and Eulophia species through micropropagation and in vitro slow growth. Also conservation attempts on Leucadendron and Mimetes species that occur in the Cape Floristic Region were attemted to increase population densities by increasing germination percentages using smoke. Furthermore, the study attempted to store seeds by assessing different cooling rates for optimising cryopreservation measures for effective conservation. The use of tissue culture to increase propagules especially critically endangered species in South African has proven to be feasible. For the trial hybrids, shoot and protocorm explants of Phalaenopsis Psychosis Pink X P. No. 1; P. Large white X P. Large pink; P. No. 1 X P. Large pink; P. Mini pink X Brighton belle; and the P. aphrodite formed clusters of protocorms and shoots when cultured on ½ strength MS media supplemented with 10, 20 and 30gL-1 banana extract or ½ strength Murashige and Skoog, (1962) (MS) media supplemented with peptone. Continuous protocorms formation could therefore be obtained by culturing endangered Disa and Eulophia shoots and protocorms on banana containing media. Plantlet conversion from somatic embryos produced on 10gL-1 banana extract enriched media was successfully achieved on ½ strength MS supplemented with 20gL-1 sucrose and no plant growth regulators in the medium. However, optimum rooting was achieved on ½ strength MS supplemented with 30gL-1 of banana extract and this medium yielded the highest survival percentages for plantlet acclimatisation. Furthermore, ½ strength MS supplemented with 1gL-1 of peptone served as a stimulant for shoot development and protocorm formation. When coupled with banana extract at all stages of development, regeneration and rooting were enhanced.

The Mimulus washingtonensis complex is a group of morphologically similar species centered in the Pacific Northwest. All are rare, and most are under consideration for listing as endangered. Morphometric and pollination data were used in developing a revised taxonomy for the group. Five species and two varieties are recognized. Mimulus pulsiferae Gray is the most widespread, occurring from southern Washington to northern California. Mimulus hymenophyllus Meinke and M. jungermannioides Suksd. are cliff species endemic to river drainages in northern Oregon. Mimulus washingtonensis Gand. occurs in east-central Oregon and western Idaho, represented by the var. washingtonensis and var. ampliatus (Grant) Meinke comb. et stat. nov., respectively. Mimulus patulus Pennell is resurrected from synonymy and differentiated from M. washingtonensis on the basis of morphology, distribution, and pollination biology. This autogamous species includes var. patulus, occurring in the Snake River drainage, and var. montanus Meinke var. nov., primarily from the northern Rocky Mountains. Mimulus evanescens Meinke sp. nov. is described from an extant population in Lassen County, California, and historic collections from widely scattered stations in Oregon and Idaho. The new species is morphologically intermediate between M. breviflorus Piper and M. latidens (Gray) Greene. Calyx and leaf morphology also suggest an affinity to M. grayi Grant, M. inconspicuus Gray, and M. acutidens Greene, of cismontane California. Mimulus evanescens should be considered critically endangered, because of its limited numbers and habitat degradation. Mimulus washingtonensis is pollinated by small native bees, primarily two species of Dialictus (Halictidae). These bees were specific to Mimulus flowers but did not distinguish between flowers of M. washingtonensis and M. guttatus DC., a related species that increases with habitat disturbance. Experiments showed that mixed pollen loads diminish seed set in M. washingtonensis, since the thigmotropic stigma of that species closes permanently after any amount of conspecific pollen is applied. Although M. washingtonensis has a low pollen/ovule ratio (29.9), it is considered facultatively xenogamous based on floral morphology, stigma sensitivity, and autogamous seed set levels. Populations occurred in small, edaphically restricted patches, and they exhibited seed set reductions related to outcrossing distance. Substrate disturbance may limit reproduction in M. washingtonensis by changing population structure, threatening pollinators, and increasing competition for pollination by promoting growth and flowering of M. guttatus. / Graduation date: 1992

Thesis (M.Sc.)--Memorial University of Newfoundland, 2002. / Restricted until October 2003. Includes bibliographical references.

published_or_final_version / Zoology / Master / Master of Philosophy

Thesis (M. Env. St.)--University of Adelaide, Mawson Graduate Centre for Environmental Studies, 1993. / Includes bibliographical references (leaves 63-71).

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographic references (leaves [114]-129).

Thesis (M.S.)--Missouri State University, 2009. / "May 2009." Includes bibliographical references (leaves 12, 28-29, 39). Also available online.

Thesis (Ph.D.)--Aberdeen University, 2009. / Title from web page (viewed on July 23, 2009). Includes bibliographical references.