Orientador: Marcelo Nogueira Rossi / Banca: Aline A. Cavalari Corete / Banca: Renata Cristina Batista Fonseca / Resumo: O presente estudo foi realizado em uma Floresta Estacional Semidecidual, localizada na Estação Ecológica do Caiuá, município de Diamante do Norte, Estado do Paraná, Brasil. Para o levantamento fitossociológico, em cada uma das parcelas demarcadas, foram amostrados todos os indivíduos com perímetro a altura do peito (PAP) igual ou superior a 15 cm, totalizando 1.200 indivíduos arbóreos distribuídos em 78 espécies, 64 gêneros e 31 famílias. As famílias de maior riqueza de espécies foram Leguminosae com 14 espécies, Meliaceae com nove, Myrtaceae, Rutaceae e Euphorbiaceae com cinco espécies cada, Lauraceae com quatro e Polygonaceae, Annonaceae e Flacuortiaceae com três espécies cada. Sloanea monosperma, Gallesia integrifolia, Guarea guidonea, Trichila casaretti, Nectandra sp., Chrysophyllum gonocarpum, Guarea kunthiana, Nectandra cuspidata, Campomanesia xanthocarpa e Endlicheria paniculata foram as espécies com maior valor de importância. O índice de diversidade de Shannon (H') obtido foi de 3,37, corroborando com valores obtidos em outros estudos desenvolvidos em florestas ripárias no Noroeste do Paraná / Abstract: This study was carried out in a Semi-deciduous Seasonal Forest located in the Estação Ecológica do Caiuá, city of Diamante do Norte, State of Paraná, Brazil. All plants with DBH greater than or equal to 15 cm were sampled within each parcel, totalizing 1,200 individuals distributed within 78 species, 64 genus and 31 families. Plant families with great species richness were Leguminosae with 14 species, Meliaceae with nine, Myrtaceae, Rutaceae and Euphorbiaceae with five species each, Lauraceae with four and Polygonaceae, Annonaceae and Flacuortiaceae with three species each. Sloanea monosperma, Gallesia integrifolia, Guarea guidonea, Trichila casaretti, Nectandra sp., Chrysophyllum gonocarpum, Guarea kunthiana, Nectandra cuspidata, Campomanesia xanthocarpa and Endlicheria paniculata were those species with high levels of importance. The Shannon's diversity indices (H') was 3.37, corroborating with other studies carried out in riparian forests in the Northwest region of Paraná / Mestre

Doctor of Philosophy / Department of Biology / John M. Blair / Approximately 96% of native tallgrass prairie in North America has been lost, which accentuates the need for effective methods to restore the structure and function of these degraded ecosystems. Many prairie restorations aim to restore grass and forb species in proportions reflecting plant species diversity in native prairie. A target grass-forb species mixture is typically chosen at the onset of restoration, but often, grasses become excessively dominant and forbs are underrepresented as the community develops. Several studies have examined the potential for increasing forb cover and diversity in newly restored grasslands, but few studies have assessed factors limiting forb cover and diversity in well-established grass-dominated prairie restorations. The primary objective of this research was to assess the potential for enhancing plant species diversity and productivity in an established grass-dominated prairie restoration by selective removals of dominant grass species, and by manipulating resources (soil nutrients, light availability) or mycorrhizal interactions. A 7-year old grass-dominated restoration was used to evaluate plant and soil responses to manipulations in three separate studies. The first study examined the potential suppressive effects of dominant grasses on plant diversity by reducing the cover and biomass of two dominant grass species, Andropogon gerardii and Panicum virgatum. After 3 years, the removal of A. gerardii increased species richness and diversity, which was correlated with increased light availability, but not changes in soil resources. The second study examined the responses of restored grassland communities to long-term manipulation of soil resources (nutrient availability or soil depth), and to aboveground biomass removal via mowing. The long-term manipulation of soil resources did not alter plant species diversity, but nitrogen and light availability were important factors regulating plant productivity. The third study assessed the effects of manipulating arbuscular mycorrhizal (AM) fungi, through the use of either commercial inoculum or fungicide, on plant communities in restored prairie. Mycorrhizal suppression reduced grass productivity, suggesting that fungicide may be useful for enhancing diversity of restored prairies that are dominated by obligate mycotrophic grasses. In total, these studies suggest that competition between dominant grasses and subordinate forbs limits plant diversity in restored tallgrass prairie.

The Hantam-Tanqua-Roggeveld subregion is located within the Succulent Karoo and Fynbos Biomes, in the predominately winter rainfall area of the Northern and Western Cape Provinces. A phytosociological analysis identified and mapped eight plant associations and 25 subassociations. Forty Whittaker plots were surveyed to quantify the botanical wealth in the area. Each plant association produced its own species-area curves, with the curves of the Mountain Renosterveld and Winter Rainfall Karoo more similar to one another than to the Tanqua Karoo. Species richness was highest for Mountain Renosterveld, intermediate for Winter Rainfall Karoo and lowest for Tanqua Karoo vegetation. The Mountain Renosterveld and Winter Rainfall Karoo values for evenness, Shannon and Simpson indices were not significantly different, but these values were significantly higher than for the Tanqua Karoo. An ordination of diversity data confirmed a clear Tanqua Karoo cluster, but the Mountain Renosterveld could only be partially separated from the Winter Rainfall Karoo. Chamaephyte, cryptophyte and therophyte species dominated the study area. Comparisons of life form spectra among associations showed clear differences at a species and vegetation cover level. The percentage contribution of succulent species was low in Mountain Renosterveld, intermediate in Winter Rainfall Karoo and highest in the Tanqua Karoo. Results confirmed the Tanqua Karoo and Winter Rainfall Karoo inclusion into the Succulent Karoo Biome and the strong karroid affinities of the Mountain Renosterveld. Abandoned croplands of various ages surveyed in the Roggeveld revealed that species richness increased with age yet no similar increase in evenness, Shannon or Simpson indices was found. An abandoned cropland of approximately 33-years should be as species rich as the natural vegetation but was floristically still very different. Recovery rates of the different life forms varied across the different ages of the abandoned croplands. A ten-year post-fire study in the Mountain Renosterveld indicated that species richness and Shannon index values usually reached a maximum within three years and then declined. A Principal Co-ordinate Analysis of species compositional data separated the first two years from the following eight years. Succession seemed to follow the ‘initial floristic composition’ model of Egler (1954). / Thesis (PhD)--University of Pretoria, 2010. / Plant Science / unrestricted

Quartz fields contribute significantly to plant diversity in the Succulent Karoo biome. They are distinctly different from surrounding habitats and have high levels of plant endemism. Biological soil crusts are features of quartz field soils and fulfill a vital function in that they stabilize soils. It is important for managers of nature reserves and agricultural rangelands to know what factors influence quartz field soils and vegetation. Both stakeholders could benefit from new information that would allow for informed decision-making regarding land-use on quartz fields. The present study took place in the Namaqua National Park that contains a significant proportion of the Riethuis-Wallekraal quartz fields phytochorion. The first part of the study aimed to understand whether certain land-use activities potentially destabilize quartz field soils, which might have possible ramifications for associated biological soil crusts and vegetation. It was followed by relating variation in soil stability with species richness, cover and species composition of quartz field vegetation. Overall, positions assumed to be impacted by land-use activities had less stable soils compared to positions assumed not be impacted. Soil stability had a significant influence on species richness and cover but to a lesser degree on species composition. Quartz field vegetation was significantly influenced by soil physical and chemical properties as well as location in the quartz fields landscape. The second part of the study aimed at understanding how species richness of isolated quartz outcrops is related to their size compared to that of a mainland body of quartz outcrops. No clear species-area relationships emerged from the study. There were significant differences between isolated outcrops and mainland outcrops in substrate and vegetation composition. Findings are discussed in relation to Island Biogeography Theory.

No description available.

No description available.

Submitted in fulfilment of the requirements for the degree of Master of Applied Sciences in Biotechnology, Durban University of Technology, Durban, South Africa, 2015. / In contrast to orthodox seeds, recalcitrant seeds are short-lived, shed at relatively high water contents (WCs), and are desiccation sensitive. Presently, the only option for long-term conservation of genetic resources of such plant species is by cryostorage in liquid nitrogen (LN; -196°C) or in the vapour phase over LN (at -150⁰C to -160⁰C). A number of cryopreservation protocols applied for recalcitrant zygotic embryos or embryonic axes of tropical/sub-tropical species have reported survival as either root or shoot development or callus formation, with no shoot or root production after cryopreservation. This is a consequence of the challenges encountered when optimising the WC for successful cryopreservation across species. Other shortcomings may also be the formation of ice or the sensitivity to desiccation resulting in lethal damage or poor re-growth. However, for successful cryopreservation, a normal plantlet with a shoot and a root needs to be obtained post-cryo. Specimens required for successful cryopreservation must be small; therefore embryonic axes excised from seeds have been often used as the explants of choice. However, in some cases, excised embryonic axes of mature recalcitrant seeds are too large to be cryopreserved, or, even if small, may be adversely affected by excision, dehydration and/or immersion in LN, thus failing to produce plantlets after cryopreservation. As a result, in such cases, there is a need to develop explants alternative to zygotic axes such as buds derived from in vitro shoots, shoot meristems, or shoot apices and somatic embryos. These alternative explants must have a high capacity for plantlet formation before and after cryopreservation. The present study aimed to successfully cryopreserve shoot apices of Trichilia emetica and T. dregeana, tropical recalcitrant-seeded tree species, and monitor the responses or effects of some of the procedural steps involved in cryopreservation on the survival and shoot production from these shoot apices. The main foci of the investigation were to produce vigorous plantlets after cryopreservation and ultimately develop a protocol for the successful cryopreservation of germplasm of these species. Furthermore, this study reports on a number of factors that may affect survival after cryopreservation, viz. WC of the explants, PVS2 treatment, production of reactive oxygen species (ROS) and levels of endogenous total aqueous antioxidants (TAA) during the various steps of cryopreservation. The effects of the various steps of cryopreservation on the ultra-structure of the shoot apices were also observed. Cathodic protection (by using highly reducing cathodic water; CW) of the explants was attempted to improve vigour and shoot production from the surviving shoot apices after cryopreservation as cathodic water has been reported to ameliorate the excessive burst of ROS, which often accompanies the stresses imposed by the procedural steps of cryopreservation. Experiments were also performed to optimise the medium for vigorous shoot formation from the shoot apices. Shoot apices of T. emetica in this study had an initial WC of ca. 2.2 g g -1 dry weight (DW) upon excision. Although the WC of the shoot apices decreased slightly after cryoprotection with PVS2, it did not result in sufficient dehydration before cooling. Upon retrieval from LN, 68% of the shoot apices survived and 40% of those produced shoots. Treatment of shoot apices with CW did not improve the survival or shoot production from the apices following cryo-retrieval. This could be a direct consequence of increase in WC of the shoot apices following CW treatment. Water content is not the only factor affecting successful cryopreservation; the production of ROS and the level of antioxidants may also have an impact on regrowth after cryogen exposure. Rapid changes in temperature when the samples are cryo-stored and then rewarmed result in an increase in ROS production, which could have affected the shoot production. More importantly the antioxidant activity showed a rapid decrease during recovery, especially in the CW treated shoot apices, which might have also led to the poor survival and shoot production from the shoot apices. Ultrastructural observations showed the injurious effects of PVS2 treatment typified by derangement of plastids, development of numerous small vesicles along the cell membrane and abnormalities in the structure of the nuclear envelope in the shoot apical cells both before and after cryogen exposure. Following cryo-retrieval, the meristem cells were extensively deteriorated – indicating non-survival, however, some shoot apices had areas of surviving cells which might have led to 40% shoot production after cryopreservation. Based on the studies on optimising medium composition for shoot formation from the apices, woody plant medium (WPM) with 1 mg L-1 BAP + 0.1 mg L-1 IBA was found to be the best medium which gave a higher shoot production of 67 – 70% before cryopreservation compared with only 18 – 20% shoot formation on media used previously. Therefore, this medium was used as the recovery medium. Encapsulation-dehydration of the shoot apices and the use of PVS3 instead of PVS2 for cryoprotection were also employed in an attempt to improve the survival and shoot production after post-cryo, but both methods did not result in any shoot production although 92% and 90% of the shoot apices survived cryogen immersion, respectively. While the shoot apices of T. emetica resulted in 40% shoot production following retrieval from LN and recovery on WPM with 1 mg L-1 BAP + 0.1 mg L-1 IBA, attempts to further improve the shoot production were not successful. The results of this study suggest that the shoot apices used were possibly not sufficiently developed, and with the commensurately high WC, proved to be unsuitable explants for germplasm conservation of T. emetica. The injurious effects of PVS2 treatment both before and after cryogen exposure as observed from the ultra-structural studies provide a clue to the repeated failure to cryopreserve embryonic axes of many tropical recalcitrant-seeded species after treatment with PVS2. Maintaining mother material in culture for longer durations before explant excision in order to allow better development of the axillary buds and render the cytosol more concentrated, and optimising the exposure duration to loading solution and concentration of sucrose in the loading solution might however, provide sufficient dehydration tolerance to PVS2 leading to successful vitrification up on cooling.

The biggest threat and cause to loss of biodiversity have been found to be the intensification of agriculture under the 20th century. Messenia is one of the oldest olive cultivation areas in Greece and the landscape is dominated by olive groves characterized by extensive tillage, which causes serious erosion and might be a threat to plant diversity. Organic olive cultivation is an alternative that aims to preserve and support biodiversity. In this study the plant composition in the edge zones of an organic and a conventional olive grove in Messenia were inventoried to be able to investigate if organic cultivation methods enhance plant diversity. Moreover, other factors affecting plant diversity in olive groves and suggestions for precautions in the olive cultivation sector to support biodiversity are discussed. The results show that the organic olive grove hosted 40 % higher species richness, which indicates, as in other similar studies, that the organic olive cultivation methods have a higher capacity to support biodiversity. By restricting tillage and promoting organic olive cultivation, not only biodiversity would be enhanced; this could also prevent further soil erosion and create a more heterogenic agricultural landscape with higher biological and cultural values.

published_or_final_version / abstract / Biological Sciences / Master / Master of Philosophy

A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctor of Philosophy. 5 September 2016, Johannesburg, South Africa. / There is an urgent need to study the effects of elephants on biodiversity given the ability of megaherbivores to transform vegetation composition, structure and function by killing selected plants. Within a biodiversity framework of different aspects of diversity across different scales, we need to understand elephant effects across time and space, acknowledging disequilibrium dynamics of savannas. However, most savanna studies are conducted either over a short time frame, over a limited spatial extent, or without species compositional data. The Linyanti riparian woodland in northern Botswana represents a valuable opportunity to study the effects of elephants as it is subject to extremely high elephant concentrations in the dry season as elephants congregate on the perennial river. Moreover, because of trampling effects by large herbivores and high soil moisture, fire is largely excluded, allowing the study of intense elephant impacts in relative isolation. This PhD thesis aims to assess long-term (16-18 years) compositional and structural change at a large spatial scale (50 km of riverfront) of the Linyanti riparian woodland, built upon two earlier studies in 1992/2 and 2001. Specifically, it aims to establish the effects of elephants on 1) the spatial heterogeneity of disturbance across the woodland; 2) compositional changes of the canopy tree layer caused by elephant impacts; 3) the potential of the woodland to regenerate from seedlings; 4) structural changes due to woodland decline and shrub increase. It finally aims to synthesise these findings for biodiversity and the implications for conservation and management. Spatial heterogeneity was assessed by delineating patches of intense disturbance using the clustering algorithm DBSCAN. I manually marked dead trees within a 2000 ha overlapping riparian area from the 1992, 2001, and 2010 aerial photographs and determined these trees were significantly clustered in the landscape to form patches of disturbance. Disturbance patches were highly dynamic over the period where small patches appeared, grew and coalesced over time, whilst a few patches fragmented or disappeared. The overall dynamic was of smaller patches coalescing resulting in the total patch area increasing from 6% in 1992 to 23% in 2010. Mortality increased mostly in the inter-patch areas but the overall dead tree appearance rate of 0.28 trees.ha.yr-1 was not much higher than a background tree death rate calculated for exclosures in other areas. The slow mortality rate coupled with progressive decline suggests there was little recruitment into the canopy to replace the trees that were lost. Even though large areas remained that were not classified as disturbance patches, there was evidence of increased fragmentation where inter-patch areas became increasingly small and isolated. This increase in greater areas of disturbance represents a state shift to decreased heterogeneity although landscape patchiness still remained in 2010. Projections were that mortality rate and patch formation would decrease. To assess compositional changes, I reconstructed the pre-1992 canopy tree woodland by combining both living and dead trees in 1992, and compared this to the 1992 and 2008 woodland composition. The woodland showed progressive declines from an Acacia spp.-Colophospermum mopane dominated tall tree woodland pre-1992 to a woodland in 2008 composed primarily of two resilient species (C. mopane, Combretum hereroense), and one avoided species (Philenoptera violacea). I compiled Size Class Distributions of individual canopy tree species and compared proportional high impact on living and dead trees between 1992 and 2008. High elephant impact was defined as more than 50% stem circumference ringbarked or with the main stem or majority of side stems broken. I found that elephant impact was the likely cause of the woodland decline, although wind and natural senescence were variably important for some species. The acacias had nearly disappeared from the woodland, declining in proportional abundance from 30% in the reconstructed pre-1992 woodland to just 4% in 2008. Over time there was a progressive shift in elephant impact from abundant preferred and vulnerable species like Acacia spp. and Terminalia spp. to species more resistant to debarking like Combretum imberbe and Berchemia discolor. The abundant species C. mopane proved highly resilient to intensive elephant impact. The seedling layer (plants below 0.5m) had high proportions of canopy tree species including the acacias, and all but the rarest species were recorded. This suggests regeneration of the woodland is possible but there was a demographic bottleneck of seedling mortality with few saplings recorded over the time period. To determine the structural changes which have taken place, I separated shrub species and canopy-forming tree species and assessed density changes in the sapling (<2.5m) and tree (>2.5m) layers. Tall (>2.5m) canopy tree density decreased by half between 1992 and 2008, representing an annual loss rate of 2.7% without replacement. Except for Colophospermum mopane, there was no compensatory regeneration in the form of saplings. Colophospermum mopane was highly resilient to elephant impacts, coppicing vigorously following impact to form local ‘browsing lawns’ which may benefit other browsers. The overall shrub density increased 2.5 times while one shrub species (Combretum mossambicense) increased five-fold in density and came to constitute 50% of the total woody plant density. This shrub species increased rapidly, at an exponential growth rate of 10.5% per year, representing pervasive shrub encroachment. Its invasion wave was incipient in 1992 and by 2008 many of these plants had grown beyond 2.5 m in height, forming a dense screen. Small plants of this species <1 m in height had become sparse by 2008, suggesting that the invasion had become curtailed by then. I proposed that the spread of this shrub was due to its unpalatability by elephants, although it is an important browse species for ruminants. A potential global driver of enriched atmospheric CO2 or regional aridification could not be ruled out. The state shift from woodland towards dense shrubland caused by differential elephant impacts has potential negative consequences for structural and functional diversity. I attempted to synthesize the findings for biodiversity and concluded that there was a state shift towards pervasive disturbance with a corresponding decline in spatial heterogeneity, although composition of the disturbance patches was not studied. There has however, not been a state transformation from woodland and stands of tall trees were still present in the woodland. Coupled with the potential regeneration of the woodland from seedlings, these findings highlight the importance of long-term studies of non-equilibrium savannas. The main threat to biodiversity of the woodland was not elephant-induced mortality of large trees, but rather the lack of recruitment and the pervasive shrub encroachment of a single species. It may be, however, that alternate states of canopy trees and unpalatable shrubs exists, enhancing long-term functional diversity, provided the system remains relatively open and elephants are free to move to other areas. Ultimately the only management strategy of relatively open areas with high elephant concentrations is to accept changes and support transfrontier conservation efforts. I further assess the limitations of this study, and make recommendations for future study, specifically highlighting the need for a longer-term palaeo-ecological study to evaluate compositional changes due to episodic recruitment events. / LG2017