A 2 x 2 factorial design investigated effects of sediment nitrogen and water potassium levels on autofragment production. Reduced nitrogen levels significantly increased autofragment production whereas potassium levels did not significantly alter production. Up to 50% of autofragment production abscised from parent plants grown under low nitrogen conditions compared to 12% or less under high nitrogen conditions.

Long term storage of bauxite residue materials requires a capping stratum which will limit erosion while stabilizing slopes, limit deep infiltration of water into storage piles, and be aesthetically acceptable to the surrounding community. A diverse native vegetative community capable of surviving seasonal drought, low plant available water and nutrient constraints has the best potential of satisfying most, if not all of these requirements. Current and past rehabilitation of residue disposal areas (RDAs) using species native to southwest Western Australia has exhibited varying success. Current practices at Alcoa World Alumina Australia’s (Alcoa) Western Australia refineries have bauxite residue fines (< 150 ìm) stored within impoundments, in which the outer embankments are constructed with bauxite residue sands (> 150 ìm). The residue sands are also used as the growth media in the capping stratum for vegetation establishment on the disposal sites. Despite the inherently hostile properties of residue sand (pH > 10, EC(1:5) > 4 dS m-1, and ESP > 50), reductions in alkalinity (pH), salinity (EC) and sodicity (ESP) are corrected, through freshwater leaching, to a greater extent than is possible with residue fines. Although leaching can reduce the hostile character of residue sand, additions of phosphogypsum are typically added to expedite the removal of Na and alkalinity, and inorganic fertilizers are incorporated to improve the nutrient status of the growth media. However, due to the inconsistencies in vegetation establishment, which are attributed to poor water retention, inherent nutrient deficiencies and rapid loss of nutrients within residue sands (because of high hydraulic conductivity), further amendments are required. The aim of this study was to determine if amending the residue sand capping layer with residue fines would enhance its overall growth potential for vegetation establishment. Additions of fines to residue sands were predicted to increase water retention, add nutrients and increase the ability of the growth media to retain nutrients. Comparisons were made between the treated residue fines (seawater washed, carbonated, or unaltered) at a series of fines additions (1 - 20 % w/w) and a control(residue sand) amended with 2% (w/w) phosphogypsum and inorganic fertilizer. Comparisons were to determine the differences in 1) water retention, 2) nutrient concentrations and nutrient retention, and 3) plant growth responses and plant biomass nutrient concentrations between the growth media treatments. Four experiments were set up to measure differences in these variables which included: a glasshouse study involving the growth of Acacia saligna; a germination and emergence study (A.saligna); a two year field study in Western Australia; and a glasshouse column leaching study. Water retention increased with increasing percentage of residue fines addition. Plant available water (PAW) increased up to 110 %, with a 20 % increase in fines, representing an increase of 0.026 m3 PAW m-3 residue growth media, when compared to the residue sand only. This increase in PAW was attributed to changes in pore space distributions, due to the increasing fines contents altering the sandy texture to loamy sand texture class. However, initially much of this increase in PAW may not be realized, due to estimated temporary increases in osmotic potential associated with the high salt contents of residue sand and fines. Fines materials have much greater salt contents, and thus need to be leached to a greater extent than sands to remove the associated osmotic potential effects. Essential plant nutrients (P, K, S, Ca, Mg, and B) were increased with additions of residue fines in the glasshouse studies, although many nutrients (Mg, Zn, Mn and B) were still marginal for sustainable plant growth and development. Seawater treated residue fines additions produced the greatest increases in growth media nutrients with substantial increases in soluble (> 7 mg L-1) and exchangeable (> 0.10 cmolc kg-1) Mg, being up to 400 % greater than all other treatments. Along with these necessary nutrients, concentrations of Na were also increased in all fines additions treatments. Added Na may offset the benefits of fines additions, at least in the short term, due to inhibitions of cation uptake from Na competition. However, in the column leaching study soluble and exchangeable Na was lost rapidly from the profile, due to Ca and K displacement of Na from exchange sites during leaching. Soluble Na was removed from the profile to < 5 % of initial concentrations, after only three pore volumes of leaching, and exchangeable Na was removed from charge sites to less than 25 % of the initial concentration. Fines additions did increase concentrations of Mg and K on exchange sites, thus reflecting increased nutrient retention capacity relative to that in residue sand only. Plant growth responses and plant biomass nutrient concentrations were altered with the additions of residue fines to residue sands. The germination and emergence study demonstrated that the emergence of native seedlings was affected by additions of fines, due to the increased salinity and sodicity of the materials. Acacia saligna seedling emergence was inhibited by EC(1:5) > 2 dS m-1, which was highly correlated with a Na/Ca ratio of > 40, for all treatments, except the seawater fines additions. Seeds sown in seawater treatments emerged from growth media with EC(1:5) as high as 3.33 dS m-1 and appeared to be better correlated with Ca/Mg ratios than any other variable. Native vegetation growth responses showed mixed results in fines treated residues in the field. In the greenhouse study, plant growth tended to decrease with fines additions. Poor plant growth with increasing fines additions occurred despite the increased water retention and increased nutrients. This was attributed to the addition of Na associated with the residue fines, as increases in Na, EC and ESP in fines treatments all appear to have limited growth of Acacia saligna in the glasshouse over three months. Seawater fines additions had elevated Mg and B concentrations in plant biomass, and performed better than the carbonated or unaltered fines treatments, but still had reduced growth compared to the residue sand, which contained lower Na concentrations. Additional plant growth limitations may have occurred, due to possible plant nutrient deficiencies including: Mg, Zn, Mn and B and Na toxicity. Incorporation of residue fines into residue sands did increase water retention, nutrient concentrations and the nutrient retention capacity, but did not enhance the overall growth potential for vegetation, at least in the short term. Reductions in germination and emergence of seedlings and reduced plant growth were attributed to increases in Na concentrations introduced from the fines. Seawater washed residue fines had lower ESP and greater concentrations of nutrients, thus reducing the negative impacts from the additional Na introduced, and showed the greatest promise as a fines amendment. As leaching occurs over the first few initial months of rehabilitation, it is expected that the majority of the Na will be removed, from a system with an addition of 5 to 10 % fines, and the positive benefits of the fines additions will than be realized. Findings also illustrate that delaying the planting of vegetation on RDAs, until adequate leaching has occurred to reduce the Na concentrations, will substantially increase vegetation emergence and establishment.

Thesis (M.S.)--University of Toledo, 2009. / Typescript. "Submitted as partial fulfillment of the requirements for The Master of Science Degree in Biology (Ecology-track)." "A thesis entitled"--at head of title. Bibliography: leaves 28-32.

Thesis (Ph. D.)--University of Hong Kong, 1994. / Includes bibliographical references (leaves 275-295).

Thesis (Ph.D.)--University of Wollongong, 2004. / Typescript. Includes bibliographical references: leaf 171-191.

Thesis (MTech (Agriculture))--Cape Peninsula University of Technology, 2017. / Cowpea (Vigna unguiculata (L.) Walp) is an important grain and fodder legume grown around the world. It is a dual purpose grain legume crop, providing food for man and livestock. Cowpea is identified as a potential crop to diversify food production, minimize production input by improving soil fertility and improve micronutrients of seed, therefore, improving human nutrition. There is limited information available on cowpea production and suitable agronomic practices including planting date to best suit different environmental conditions in South Africa. The objective of this study was therefore to i) evaluate two soil types (sandy and clay soil) and its effect on cowpea, yield components and mineral composition, ii) the effect of different planting date and iii) assess the effect of zinc fertilizer application rate on vegetative, reproductive parameters and mineral content of cowpea seed. A field trial was conducted in Agricultural Research Council (ARC), in two locations Nietvoorbij (clay loam soil) and Bien Donne’ (sandy soil) during the 2015 summer planting season. The trial layout was conducted in a randomised complete block design (RCBD) with five replicates. The factors of the study include three cowpea lines: Cowpea Veg1, M217 and Qukawa with zinc application rate of (0, 15 and 30 kg/ha) through soil application and two planting date (2 October and 2 November 2015). The following agronomic variables were collected, in both locations: germination rate, number of leaves, number of branches, plant height, number of seed per pod, number of pods per plant, pod length, pods per treatment, pod weight, 100 seed weight, morphological traits, moisture content and seed mineral content. Vegetative data was collected on a fourth-night basis on six middle plants per treatment and reproductive parameters were taken after harvest. The variables were subjected to ANOVA using software SAS (2012). Treatments were tested at 5% level of significance and differences between treatments were separated using LSD and DMRT of the SAS 2012 test. The results indicated that vegetative and reproductive parameters measured varied significantly among cowpea lines in each location and across locations due to different cowpea lines and soil type. Line Cowpea Veg1 and Qukawa were the best performing line in both vegetative and yield parameters across the two planting dates in 2015. These lines significantly obtained higher plant height than line M217. Yield and yield parameters were significantly affected by cowpea line. Qukawa obtained the highest seed yield at Bien Donne’ with a mean of 1184.2 kg/ha and seed yield of 686.25 kg/ha for Cowpea Veg1 at Nietvoorbij. The second planting date (2 November 2015) improved germination of plants across the two locations, therefore improving vegetative growth. Zinc (Zn) fertilizer significantly improved plant height across all treatments. An inconsistent response to yield parameters due to Zn application rate was observed. However, though not significant, Zn application of 15 kg/ha increased most of the measured parameters. It was concluded that line Cowpea Veg1 and Qukawa were the best performing lines. The second planting date (2 November) increase germination rate for both locations. It is therefore, recommended that future research should evaluate Zn fertilizer time of application.

Thesis (MTech (Horticulture))--Cape Peninsula University of Technology, 2017 / Transpiration serves in leaf cooling, maintaining turgor pressure, promoting xylem transport of nutrient solutes from roots to shoots and delivering mobile soil nutrients to root surfaces. Soil availability of nitrogen can modulate transpiration rates, consequently powering nutrient delivery to the root surfaces (‗mass-flow'). Although such knowledge on N-regulation of transpiration is available, it remains unknown, however, whether it is NO3- or NH4+ that regulates transpiration. Given that both nitrogen forms co-occur in soils, it is not known how they interact at varying ratios in modulating stomatal behaviour. To test the functional role of NO3- and NH4+ in regulating water fluxes for mass-flow nutrient acquisition, P. vulgaris L. plants were grown with NO3- or NH4+ placed at one of four distances behind a nylon mesh, which prevented direct root access to nitrogen, whilst control plants intercepted the nitrogen source (Chapter 3). Day- and night-time stomatal conductance and transpiration, measured using Infra-Red Gas Analyser (IRGA) declined in NO3- fed plants with the increased distance behind a nylon mesh, with maximum water fluxes at the closest distance (ca. 0 mm), demonstrating a regulatory role of NO3- on stomata closure. An opposite trend was displayed by NH4+ -fed plants, which indicated the incapacity of NH4+ to down-regulate water fluxes and ammoniacal syndrome at high concentrations. To test how different [NO3-] and [NH4+] regulate day- and night-time stomatal conductance and transpiration (Chapter 4), P. vulgaris was fed with six concentrations (0, 0.25, 0.5, 1, 2, 4 and 8 mM) of each nitrogen form. A biphasic trend emerged, as postulated in previous studies (Wilkinson et al., 2007; Matimati et al., 2013), characterized by an increase in stomatal conductance and transpiration as [NO3-] increased, attaining a maximum before declining with higher [NO3-]. Plants displayed 2-fold higher photosynthetic rates, 2.2-fold higher stomatal conductance and 2.3-fold higher transpiration rates at 4 mM than at 0.25 mM of [NO3-]. The lowest [NO3-] up-regulated night-time stomatal conductance and transpiration, indicating that NO3- -fed plants opened their stomata at night-time, but reduced night-time water loss at higher [NO3-]. NH4+-fed plants had the incapacity to regulate day- and night-time water fluxes, but rather displayed wilting and stress known as ‗ammoniacal syndrome'. Thus, under NO3- deprived soil conditions P. vulgaris may be opportunistic in their water uptake, transpiring more when water is available in order to draw nutrients through ‗mass-flow'. This thesis explored and confirmed the functional role of NO3- in regulating day- and night-time water fluxes as a mechanism for increasing ‗mass-flow' acquisition of N and possibly other nutrients, signalling a down-regulation of day-time and night-time water fluxes when [NO3-] is replete (Chapter 3 & 4). Where both NO3- and NH4+ are present in soils, it is the [NO3-] and not [NH4+] that regulated stomatal conductance and transpiration. Since organic nitrogen forms such as amino acids also occur in soils, there is a need for further work on their role in stomatal behaviour. Using amino acids laced with 15N isotopes as a nitrogen source can allow their acquisition and role on stomatal behaviour to be discovered. Current trends in research are focussed around developing real-time in-situ sensing of soil nitrogen status to promote enhanced nitrogen and water use efficiency in agricultural systems. This thesis provides the vital literature on stomatal regulation by [NO3-].

Plant growth and survival are affected by the nutrients available in the environment. Nitrogen (N) is most often the limiting nutrient in terrestrial ecosystems, particularly in temperate and boreal forests, such as those on Vancouver Island. To overcome the challenge of limited nutrient availability, plants have evolved symbiotic relationships with fungi, called mycorrhizae. While research on the importance of mycorrhizal symbioses for N uptake by plants continues to grow, we have a limited understanding of the mechanisms of N uptake and transfer by mycorrhizae. This knowledge is crucial to fully understand N uptake and assimilation by plants. This study aimed to determine the influence of soil N availability on conifer growth and foliar N content, and on the N form preferences and sporocarp N content of associated mycorrhizae. Inorganic and organic soil N production was determined for two sites, Fairy Lake and San Juan, near Port Renfrew British Columbia, under pure plantations of Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco), Sitka spruce (Picea sitchensis [Bong.] Carr.), western redcedar (Thuja plicata Donn ex D. Don in Lamb) and western hemlock (Tsuga heterophylla [Raf.] Sarg.). Ammonium, nitrate and amino acid production contrasted between the sites, with relatively higher N production in San Juan compared to Fairy Lake. This indicated differences in soil N cycling, most likely due to differences in moisture and topography. In general, conifer species did not affect inorganic and organic soil N production. Growth of conifers increased with increasing N availability, and differed between species, with Douglas-fir and Sitka spruce having the greatest growth and western redcedar having the least growth. Foliar %N and 15N were found to differ among the conifer species, and western redcedar had the lowest foliar N concentrations. While site quality was not reflected in foliar %N, foliar 15N was found to increase with increasing 15N of the forest floor. Ectomycorrhizal (ECM) sporocarps reflected site quality, with greater N concentrations but lower 15N values on the higher N site. Sporocarp 15N concentrations were higher than foliar 15N concentrations, suggesting N isotope fractionation by mycorrhizae. Finally, site N availability was not related to the rates of N form uptake by ECM genera. Both ECM and arbuscular mycorrhizae (AM) did not have substantial nitrate uptake, despite a greater supply of nitrate. Ammonium was found to be taken up at higher rates than nitrate in the ECM and AM roots, suggesting a preference for ammonium, possibly due to ammonium being energetically cheaper to metabolize and suppressing nitrate transporters in mycorrhizal fungi. Differences in proportions of N form uptake and sporocarp N content among ECM genera were seen, indicating potential niche formation based on functional traits such as N form uptake and mycelial morphology. Knowing how mycorrhizae respond to different N forms and rates of N supply will not only increase our knowledge of N dynamics in mycorrhizal symbioses, but will help predict the effects environmental changes, such as disturbance and N deposition, may have on these systems. / Graduate / 2018-08-09

The effects of seed size on sprout growth of potato seed pieces were investigated in controlled temperature conditions. The objectives were to quantify the effects of seed size, plant growth regulators (gibberellin and paclobutrasol) and calcium availability on sprout length, sprout and root dry mass, as well as the fraction of seed reserves utilised for sprout development. The interactive effect between seed size and temperature was also investigated Sprout length did not differ among seed pieces ranging from 1 to 8g in mass. For larger seed pieces sprout length, dry mass and dry mass per unit length, and the root dry mass increased with increasing seed size, reaching a maximum at a seed mass of 24g after 40 days. The smaller the initial seed piece the larger was the fraction of assimilates utilised for sprout growth. Sprout length and dry mass as well as assimilate mobilisation for growth, were promoted by dipping seed pieces in gibberellin solutions, with the greatest effect obtained with 30 ppm GA, the highest concentration used in the experiment. Paclobutrasol showed inhibiting effects on all growth parameters except for sprout thickness. The root dry mass did not differ among treatments. Sprout growth was enhanced by calcium availability, with the greatest effect obtained with exogenous supply of calcium at 10 mmol/I as CaS04. A similar effect was noticed when complete nutrient solution was supplied. CaC03 was less effective than CaS04. Calcium availability to sprout tips affected sprout growth only when the roots were deprived of nutrients. Sprout length of the two seed pieces (3 and 5g in mass) was similarly affected by temperature over time, with the sprouts at the low temperature initially shorter, but reaching the same lengths as those at the high temperature after 30 and 40 days. Sprout dry mass and thickness of 3 and 5g seed pieces were similarly affected by temperature, with higher dry mass and greater thickening at 16°C than at 26°C. Exposure to low temperature (16°C) was associated with the transfer of a large fraction of seed reserves to the sprouts. / Dissertation (MSc (Agric): Agronomy)--University of Pretoria, 2006. / Plant Production and Soil Science / unrestricted

A study was conducted to develop, demonstrate and assess the Pour-through nutrient extraction procedure for bedding flats and plug trays. The Pour-through technique involves pouring a prescribed volume of water on a recently irrigated medium, then collecting and analyzing the leachate to predict nutrient availability in the medium. The volume of water necessary to collect a Pour-through from a 1204 bedding flat was determined to be 5 ml per cell based on leachate pH and electrical conductivity (EC) at various volumes of water applied. Impatiens wallerana ‘Super Elfin Red’ and Tagetes erecta ‘Apollo’ were grown in 1204 bedding flats at three fertilizer concentrations to assess the overall potential of the Pour-through procedure. Analysis of leachate EC, pH, and macro- and micro-nutrients indicated that the Pour- through method of nutrient extraction was sensitive and effective in extracting available nutrients. Leachate analysis was positively correlated to fertilizer nitrogen concentration, shoot tissue dry weight, and nutrient concentrations of conventional Saturated Media Extract methods. Preliminary studies indicated that evenly applying 200 ml of water per plug tray cells produced adequate leachate (50 ml) for laboratory analysis of EC, pH, and macro- and micro-nutrients. Impatiens wallerana ‘Super Elfin Red’ and Tagetes erecta ‘Apollo’ were grown in plug trays at three fertilizer concentrations to assess the overall potential of the Pour-through procedure. Analysis of leachate EC, pH, and macro- and micro-nutrients indicated that the Pour-through method of nutrient extraction was sensitive and effective in extracting available nutrients. Pour-through leachate analysis was positively correlated to fertilizer nitrogen concentration and to whole shoot tissue. / Master of Science