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Structure-function analysis of phototropin receptor kinasesJones, Matthew A. January 2008 (has links)
Thesis (Ph.D.) - University of Glasgow, 2008. / Ph.D. thesis submitted to the Faculty of Law, Business and Social Sciences, Department of Economics and Social History, University of Glasgow, 2008. Includes bibliographical references. Print version also available.
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The role of red and blue light in leaf and cotyledon expansion /Blum, Dale, January 1998 (has links)
Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [105]-116).
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The role of secondary photoreceptors in phototropism in Arabidopsis and the isolation and characterization of mutants altered in the enhancement of phototropismStowe-Evans, Emily L. January 2001 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 231-256). Also available on the Internet.
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The role of secondary photoreceptors in phototropism in Arabidopsis and the isolation and characterization of mutants altered in the enhancement of phototropism /Stowe-Evans, Emily L. January 2001 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 231-256). Also available on the Internet.
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Effects of photo-selective nets on tomato (Solanum lycopersicum) plant growth and fruit quality at harvest.Tinyane, PhushudI Peter. January 2013 (has links)
M. Tech. Agriculture. / Investigates the effect of photo-selective nets on plant growth parameters (plant height, leaf area, leaf chlorophyll, stem diameter, flowering intensity, and fruit settings), pest and disease incidence, and yield (number of fruits per plant) in three selected cultivars.Secondly, to investigate the effect of shading on fruit quality parameters (fruit mass, firmness, colour, soluble solids content, and titratable acidity) and health promoting compounds (ascorbic acid, lycopene content, β-carotene, total phenols and flavonoids, and antioxidant scavenging activity) in three selected tomato cultivars.
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Study of light dependent Arabidopsis phytochrome A signal transduction through FHY1 and its downstream gene expression regulationZhou, Zhenzhen. January 2009 (has links)
Thesis (Ph. D.)--State University of New York at Binghamton, Department of Biological Sciences, 2009. / Includes bibliographical references.
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Spa1 a protein involved with photoresponses incited by red and green light /McCoshum, Shaun Michael. January 2009 (has links)
Title from first page of PDF document. Includes bibliographical references (p. 26-29).
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Molecular cloning of the soybean phototropinsRoy, Pallabi January 2014 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / The phototropin photoreceptors are important regulators of plant growth and development and can therefore affect the photosynthetic activity of plants. Phototropin1 and Phototropin2 are versatile protein kinases that become activated when exposed to blue light. Their photobiological actions are best understood in the model plant Arabidopsis thaliana, where they are known to trigger several responses to blue light, one of which is phototropism, the bending of plant organs towards light. Additionally, phot1 and phot2 drive stomatal opening, chloroplast arrangement in leaf cells, leaf expansion, and leaf orientation. The phot1-specific response is rapid inhibition of hypocotyl growth, leaf positioning and mRNA stability whereas phot2 mediates the chloroplast avoidance response to high light. These responses impact a plant’s ability to capture light for photosynthesis, therefore the phototropins play important roles in optimizing a plant’s photosynthetic activity.
Soybean (Glycine max) is a very important crop plant in Indiana known for its nutritional versatility and is also utilized for biodiesel production.In spite of soybean being a key crop, there is currently no information about the functionality of soybean phototropins.
Also, being a legume, soybean has many structural and functional features that are not present in Arabidopsis. Interestingly, PsPHOT1A (a photoreceptor from garden pea) was found to be a functional phototropin as it was able to complement the phot1 mutation in Arabidopsis. The roles of these proteins in soybean will be elucidated based on the hypothesis that soybean phototropins play essential roles in regulating photosynthetic activity as do the Arabidopsis phototropins.
To date, five soybean phototropins, 3 PHOT1s and 2 PHOT2s, are believed to exist. These GmPHOT protein coding regions were amplified by RT-PCR and cloned into pCR8/TOPO or pENTR-D/TOPO vectors via TOPO cloning to utilize Gateway cloning technology to create plant transformation constructs subsequently. The cloned GmPHOT cDNAs from each of the 5 GmPHOTs were sequenced and compared to the GmPHOT sequences from the Phytozome database to assess the accuracy of the gene models. The gene models of all the GmPHOTs were found to be accurate except that of GmPHOT1B-2. The high level of sequence identity between the GmPHOTs and AtPHOTs and the conservation of LOV domains and catalytic domains indicate structural resemblance between them. This suggests that soybean phototropins should encode active photoreceptors. The cloned protein coding regions from soybean were then recombined into a plant expression vector via Gateway technology,which were then used for transformation of Agrobacterium tumefaciens. These plant expression constructs will be utilized in the future to determine the functionality of soybean phototropins in Arabidopsis.
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